دورية أكاديمية
أعرض في EDS

Irreversible Electroporation of the Pancreas Using Parallel Plate Electrodes in a Porcine Model: A Feasibility Study.

التفاصيل البيبلوغرافية
العنوان: Irreversible Electroporation of the Pancreas Using Parallel Plate Electrodes in a Porcine Model: A Feasibility Study.
المؤلفون: Rombouts SJ; Department of Surgery, University Medical Center Utrecht Cancer Center, Utrecht, the Netherlands., Nijkamp MW; Department of Surgery, University Medical Center Utrecht Cancer Center, Utrecht, the Netherlands., van Dijck WP; Department of Surgery, University Medical Center Utrecht Cancer Center, Utrecht, the Netherlands., Brosens LA; Department of Pathology, University Medical Center Utrecht, Utrecht, the Netherlands., Konings M; Department of Innovation research, University Medical Center Utrecht, Utrecht, the Netherlands., van Hillegersberg R; Department of Surgery, University Medical Center Utrecht Cancer Center, Utrecht, the Netherlands., Borel Rinkes IH; Department of Surgery, University Medical Center Utrecht Cancer Center, Utrecht, the Netherlands., Hagendoorn J; Department of Surgery, University Medical Center Utrecht Cancer Center, Utrecht, the Netherlands., Wittkampf FH; Department of Cardiology, University Medical Center Utrecht, Utrecht, the Netherlands., Molenaar IQ; Department of Surgery, University Medical Center Utrecht Cancer Center, Utrecht, the Netherlands.
المصدر: PloS one [PLoS One] 2017 Jan 04; Vol. 12 (1), pp. e0169396. Date of Electronic Publication: 2017 Jan 04 (Print Publication: 2017).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Electroporation/*methods , Pancreas/*surgery, Ablation Techniques ; Animals ; Electricity ; Electrodes ; Feasibility Studies ; Models, Animal ; Pancreas/cytology ; Sus scrofa
مستخلص: Background: Irreversible electroporation (IRE) with needle electrodes is being explored as treatment option in locally advanced pancreatic cancer. Several studies have shown promising results with IRE needles, positioned around the tumor to achieve tumor ablation. Disadvantages are the technical difficulties for needle placement, the time needed to achieve tumor ablation, the risk of needle track seeding and most important the possible occurrence of postoperative pancreatic fistula via the needle tracks. The aim of this experimental study was to evaluate the feasibility of a new IRE-technique using two parallel plate electrodes, in a porcine model.
Methods: Twelve healthy pigs underwent laparotomy. The pancreas was mobilized to enable positioning of the paddles. A standard monophasic external cardiac defibrillator was used to perform an ablation in 3 separate parts of the pancreas; either a single application of 50 or 100J or a serial application of 4x50J. After 6 hours, pancreatectomy was performed for histology and pigs were terminated.
Results: Histology showed necrosis of pancreatic parenchyma with neutrophil influx in 5/12, 11/12 and 12/12 of the ablated areas at 50, 100, and 4x50J respectively. The electric current density threshold to achieve necrosis was 4.3, 5.1 and 3.4 A/cm2 respectively. The ablation threshold was significantly lower for the serial compared to the single applications (p = 0.003). The content of the ablated areas differed between the applications: areas treated with a single application of 50 J often contained vital areas without obvious necrosis, whereas half of the sections treated with 100 J showed small islands of normal looking cells surrounded by necrosis, while all sections receiving 4x 50 J showed a homogeneous necrotic lesion.
Conclusion: Pancreatic tissue can be successfully ablated using two parallel paddles around the tissue. A serial application of 4x50J was most effective in creating a homogeneous necrotic lesion.
Competing Interests: The authors have declared that no competing interests exist.
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تواريخ الأحداث: Date Created: 20170105 Date Completed: 20170809 Latest Revision: 20181113
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC5213381
DOI: 10.1371/journal.pone.0169396
PMID: 28052102
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0169396