دورية أكاديمية
A novel peroxidase purified from Marsdenia megalantha latex inhibits phytopathogenic fungi mediated by cell membrane permeabilization.
| العنوان: | A novel peroxidase purified from Marsdenia megalantha latex inhibits phytopathogenic fungi mediated by cell membrane permeabilization. |
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| المؤلفون: | Oliveira HP; Department of Biochemistry and Molecular Biology, Federal University of Ceara, 60440-900 Fortaleza, CE, Brazil; University of International Integration of the Afro-Brazilian Lusophony, 62790-000 Redençao, CE, Brazil., Silva RGG; Department of Biochemistry and Molecular Biology, Federal University of Ceara, 60440-900 Fortaleza, CE, Brazil., Oliveira JTA; Department of Biochemistry and Molecular Biology, Federal University of Ceara, 60440-900 Fortaleza, CE, Brazil., Sousa DOB; Department of Biochemistry and Molecular Biology, Federal University of Ceara, 60440-900 Fortaleza, CE, Brazil., Pereira ML; Department of Biochemistry and Molecular Biology, Federal University of Ceara, 60440-900 Fortaleza, CE, Brazil., Souza PFN; Department of Biochemistry and Molecular Biology, Federal University of Ceara, 60440-900 Fortaleza, CE, Brazil., Soares AA; Department of Biology, Federal University of Ceara, 60440-900 Fortaleza, CE, Brazil., Gomes VM; Center of Bioscience and Biotechnology, State University of North Fluminense Darcy Ribeiro, 28013-602 Campos dos Goytacazes, RJ, Brazil., Monteiro-Moreira ACO; School of Pharmacy, University of Fortaleza, 60811-905 Fortaleza, CE, Brazil., Moreno FBMB; School of Pharmacy, University of Fortaleza, 60811-905 Fortaleza, CE, Brazil., Vasconcelos IM; Department of Biochemistry and Molecular Biology, Federal University of Ceara, 60440-900 Fortaleza, CE, Brazil. Electronic address: imvasco@ufc.br. |
| المصدر: | International journal of biological macromolecules [Int J Biol Macromol] 2017 Mar; Vol. 96, pp. 743-753. Date of Electronic Publication: 2017 Jan 03. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Guildford, Eng., IPC Science and Technology Press. |
| مواضيع طبية MeSH: | Cell Membrane Permeability/*drug effects , Fusarium/*drug effects , Latex/*chemistry , Marsdenia/*chemistry , Peroxidase/*isolation & purification , Peroxidase/*pharmacology , Plants/*microbiology, Amino Acid Sequence ; Antifungal Agents/chemistry ; Antifungal Agents/isolation & purification ; Antifungal Agents/metabolism ; Antifungal Agents/pharmacology ; Enzyme Inhibitors/pharmacology ; Enzyme Stability ; Fusarium/cytology ; Fusarium/metabolism ; Fusarium/physiology ; Hydrogen-Ion Concentration ; Kinetics ; Metals/pharmacology ; Microbial Viability/drug effects ; Molecular Weight ; Peroxidase/antagonists & inhibitors ; Peroxidase/chemistry ; Reactive Oxygen Species/metabolism ; Salicylic Acid/pharmacology ; Spores, Fungal/drug effects ; Spores, Fungal/growth & development ; Substrate Specificity ; Temperature |
| مستخلص: | An antifungal class III peroxidase was purified from Marsdenia megalantha latex (named Mo-POX) using DEAE-cellulose and gel filtration chromatography on a Superose 12 HR 10/30 column. Mm-POX has an apparent molecular mass of 67.0kDa and a pI of 5.2, shares identity with other peroxidases, and follows Michaelis-Menten kinetics. It has a high affinity for guaiacol and hydrogen peroxide. The pH and temperature optima for Mm-POX were 5.0-7.0 and 60°C, respectively. The catalytic activity of Mm-POX was decreased in the presence of classic peroxidase inhibitors including azide, dithiothreitol, ethylenediamine tetraacetic acid, and sodium metabisulfite and high concentrations of Na + , Mn + , and salicylic acid. In contrast, Ca + and Mg + , even at low concentrations, enhanced the Mm-POX enzymatic activity. This protein inhibited the germination of the conidia of the phytopathogenic fungi Fusarium oxysporum and Fusarium solani by acting through a membrane permeabilization mechanism. Mm-POX also induced oxidative stress in F. solani. Mm-POX is the first enzyme to be isolated from the M. megalantha species and it has potential use in the control of plant disease caused by important phytopathogenic fungi. This adds biotechnological value to this enzyme. (Copyright © 2017 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Antifungal protein; Fusarium; Latex; Marsdenia megalantha; Peroxidase; Protein defense |
| المشرفين على المادة: | 0 (Antifungal Agents) 0 (Enzyme Inhibitors) 0 (Latex) 0 (Metals) 0 (Reactive Oxygen Species) EC 1.11.1.7 (Peroxidase) O414PZ4LPZ (Salicylic Acid) |
| تواريخ الأحداث: | Date Created: 20170107 Date Completed: 20170417 Latest Revision: 20180525 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.ijbiomac.2016.12.083 |
| PMID: | 28057569 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0003 |
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| DOI: | 10.1016/j.ijbiomac.2016.12.083 |