دورية أكاديمية
Acidic pH with coordinated reduction of basic fibroblast growth factor maintains the glioblastoma stem cell-like phenotype in vitro.
| العنوان: | Acidic pH with coordinated reduction of basic fibroblast growth factor maintains the glioblastoma stem cell-like phenotype in vitro. |
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| المؤلفون: | Haley EM; Department of Chemical and Biological Engineering, The University of Alabama, Tuscaloosa, AL 35487-0203, USA., Tilson SG; Department of Chemical and Biological Engineering, The University of Alabama, Tuscaloosa, AL 35487-0203, USA., Triantafillu UL; Department of Chemical and Biological Engineering, The University of Alabama, Tuscaloosa, AL 35487-0203, USA., Magrath JW; Department of Chemical and Biological Engineering, The University of Alabama, Tuscaloosa, AL 35487-0203, USA., Kim Y; Department of Chemical and Biological Engineering, The University of Alabama, Tuscaloosa, AL 35487-0203, USA. Electronic address: ykim@eng.ua.edu. |
| المصدر: | Journal of bioscience and bioengineering [J Biosci Bioeng] 2017 May; Vol. 123 (5), pp. 634-641. Date of Electronic Publication: 2017 Jan 04. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Society for Biotechnology, Japan Country of Publication: Japan NLM ID: 100888800 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1347-4421 (Electronic) Linking ISSN: 13474421 NLM ISO Abbreviation: J Biosci Bioeng Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2003-: Osaka, Japan : Society for Biotechnology, Japan Original Publication: Osaka, Japan : Amsterdam, The Netherlands : Society for Bioscience and Bioengineering, Japan ; Distributed outside Japan by Elsevier Science, 1999- |
| مواضيع طبية MeSH: | Brain Neoplasms/*pathology , Fibroblast Growth Factor 2/*metabolism , Glioblastoma/*pathology , Neoplastic Stem Cells/*pathology, Acids/chemistry ; Brain Neoplasms/metabolism ; Cell Line, Tumor ; Cell Proliferation/drug effects ; Fibroblast Growth Factor 2/pharmacology ; Glioblastoma/metabolism ; Humans ; Hydrogen-Ion Concentration ; Phenotype |
| مستخلص: | Glioblastoma stem cells (GSCs) are a unique subpopulation of cells within glioblastoma multiforme (GBM) brain tumors that possess the ability to self-renew and differentiate into bulk tumor cells. GSCs are resistant to currently available treatments and are the likely culprit behind tumor relapse in GBM patients. However, GSCs are currently inaccessible to the larger scientific community because obtaining a sufficient number of GSCs remains technically challenging and cost-prohibitive. Thus, the objective of this study was to develop a more efficient GSC culture strategy that results in a higher cell yield of GSCs at a lower cost. We observed that the basic fibroblast growth factor (bFGF) is indispensable in allowing GSCs to retain an optimal stem cell-like phenotype in vitro, but little change was seen in their stemness when grown with lower concentrations of bFGF than the established protocol. Interestingly, a dynamic fluctuation of GSC protein marker expression was observed that corresponded to the changes in the bFGF concentration during the culture period. This suggested that bFGF alone did not control stem cell-like phenotype; rather, it was linked to the fluctuations of both bFGF and media pH. We demonstrated that a high level of stem cell-like phenotype could be retained even when lowering bFGF to 8 ng/mL when the media pH was simultaneously lowered to 6.8. These results provide the proof-of-concept that GSC expansion costs could be lowered to a more economical level and warrant the use of pH- and bFGF-controlled bioprocessing methodologies to more optimally expand GSCs in the future. (Copyright © 2016 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Acidic pH; Basic fibroblast growth factor; Cancer stem cells; Glioblastoma multiforme; Glioblastoma stem cells |
| المشرفين على المادة: | 0 (Acids) 103107-01-3 (Fibroblast Growth Factor 2) |
| تواريخ الأحداث: | Date Created: 20170109 Date Completed: 20170509 Latest Revision: 20181202 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.jbiosc.2016.12.006 |
| PMID: | 28063758 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1347-4421 |
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| DOI: | 10.1016/j.jbiosc.2016.12.006 |