دورية أكاديمية
[Biological microchip for establishing the structure of fusion transcripts involving MLL in children with acute leukemia].
| العنوان: | [Biological microchip for establishing the structure of fusion transcripts involving MLL in children with acute leukemia]. |
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| المؤلفون: | Nasedkina TV; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia.; Rogachev Federal Clinical Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, 119117 Russia.; nased@eimb.ru., Ikonnikova AY; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia., Tsaur GA; Regional Children's Clinical Hospital no. 1, Yekaterinburg, 620149 Russia.; Institute of Medical Cell Technologies, Yekaterinburg, 620026 Russia.; Yeltsin Ural Federal University, Yekaterinburg, 620002 Russia., Karateeva AV; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia., Ammour YI; Rogachev Federal Clinical Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, 119117 Russia., Avdonina MA; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia., Karachunskii AI; Rogachev Federal Clinical Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, 119117 Russia., Zasedatelev AS; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia. |
| المصدر: | Molekuliarnaia biologiia [Mol Biol (Mosk)] 2016 Nov-Dec; Vol. 50 (6), pp. 968-977. |
| نوع المنشور: | Journal Article |
| اللغة: | Russian |
| بيانات الدورية: | Publisher: Izdatelstvo Nauka Country of Publication: Russia (Federation) NLM ID: 0105454 Publication Model: Print Cited Medium: Print ISSN: 0026-8984 (Print) Linking ISSN: 00268984 NLM ISO Abbreviation: Mol Biol (Mosk) Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Moskva : Izdatelstvo Nauka |
| مواضيع طبية MeSH: | Gene Expression Regulation, Leukemic* , Transcription, Genetic*, Gene Expression Profiling/*instrumentation , Histone-Lysine N-Methyltransferase/*biosynthesis , Leukemia, Myeloid, Acute/*metabolism , Myeloid-Lymphoid Leukemia Protein/*biosynthesis , Oligonucleotide Array Sequence Analysis/*instrumentation , Oncogene Proteins, Fusion/*biosynthesis , Precursor Cell Lymphoblastic Leukemia-Lymphoma/*metabolism, Child ; Child, Preschool ; Female ; Gene Expression Profiling/methods ; Histone-Lysine N-Methyltransferase/genetics ; Humans ; Infant ; Infant, Newborn ; Leukemia, Myeloid, Acute/genetics ; Male ; Myeloid-Lymphoid Leukemia Protein/genetics ; Oligonucleotide Array Sequence Analysis/methods ; Oncogene Proteins, Fusion/genetics ; Precursor Cell Lymphoblastic Leukemia-Lymphoma/genetics |
| مستخلص: | MLL is involved in fusion genes with more than 100 partner genes, approximately 80 of which have been characterized at the molecular level. MLL fusion genes are often found in infants (60-80% of acute lymphoblastic leukemia (ALL) cases and 40-50% of acute myeloblastic leukemia (AML) cases) and are appreciably rarer (8-10%) in children older than 1 year of age. MLL rearrangements are important markers in diagnosis and treatment choice. To identify the partner gene is of primary importance for prognosis and minimal residual disease monitoring. The structure of the fusion gene, including localization of the MLL breakpoints, is also informative. A method was developed to examine the fusion transcripts in order to identify the partner gene among the six most common ones and to establish the exon structure of the rearranged MLL. The method includes a multiplex reverse transcriptase-polymerase chain reaction (RT-PCR) to amplify and to fluorescently label a fusion transcript fragment and subsequent hybridization of the product on a biological microchip with immobilized oligonucleotides complementary to exons of MLL and its partner genes AFF1, MLLT1, MLLT3, MLLT4, MLLT10, and ELL. Hybridization results were verified by sequencing the RT-PCR products and, in some cases, performing long-distance inverse PCR (LDI-PCR). The study involved 38 bone marrow samples from ALL patients (including 33 children younger than 1 year of age) and 15 samples from AML patients (including 10 from children younger than 1 year of age). The main partner genes were AFF1 (49%), MLLT1 (27%), MLLT3 (12%), and MLLT10 (12%) in ALL and MLLT3 (80%), MLLT10 (10%), and MLLT4 (10%) in AML. Fusion gene transcripts most commonly included MLL exon 11 (58% of ALL cases and 50% of AML cases), suggesting a breakpoint in MLL intron 11. |
| فهرسة مساهمة: | Keywords: 11q23 chromosome rearrangements; MLL gene; acute leukemia; biological microchips; breakpoints; fusion transcript |
| المشرفين على المادة: | 0 (KMT2A protein, human) 0 (Oncogene Proteins, Fusion) 149025-06-9 (Myeloid-Lymphoid Leukemia Protein) EC 2.1.1.43 (Histone-Lysine N-Methyltransferase) |
| تواريخ الأحداث: | Date Created: 20170109 Date Completed: 20170321 Latest Revision: 20190816 |
| رمز التحديث: | 20231215 |
| DOI: | 10.7868/S0026898416060148 |
| PMID: | 28064313 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0026-8984 |
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| DOI: | 10.7868/S0026898416060148 |