دورية أكاديمية
أعرض في EDS

Minocycline enhances the mesenchymal stromal/stem cell pro-healing phenotype in triple antimicrobial-loaded hydrogels.

التفاصيل البيبلوغرافية
العنوان: Minocycline enhances the mesenchymal stromal/stem cell pro-healing phenotype in triple antimicrobial-loaded hydrogels.
المؤلفون: Guerra AD; School of Pharmacy, Division of Pharmaceutical Sciences, Pharmacy Practice Division, University of Wisconsin-Madison, 777 Highland Avenue, Madison, WI 53705, USA. Electronic address: aguerra@wisc.edu., Rose WE; School of Pharmacy, Division of Pharmaceutical Sciences, Pharmacy Practice Division, University of Wisconsin-Madison, 777 Highland Avenue, Madison, WI 53705, USA. Electronic address: warren.rose@wisc.edu., Hematti P; School of Medicine and Public Health, Department of Medicine, Wisconsin Carbone Cancer Center, University of Wisconsin-Madison, 1685 Highland Avenue, Madison, WI 53705, USA. Electronic address: pxh@medicine.wisc.edu., Kao WJ; School of Pharmacy, Division of Pharmaceutical Sciences, Pharmacy Practice Division, University of Wisconsin-Madison, 777 Highland Avenue, Madison, WI 53705, USA; College of Engineering, Department of Biomedical Engineering, University of Wisconsin-Madison, 1415 Engineering Drive, Madison, WI 53706, USA; School of Medicine and Public Health, Department of Surgery, University of Wisconsin-Madison, 1685 Highland Avenue, Madison, WI 53705, USA. Electronic address: WJKao@wisc.edu.
المصدر: Acta biomaterialia [Acta Biomater] 2017 Mar 15; Vol. 51, pp. 184-196. Date of Electronic Publication: 2017 Jan 07.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: England NLM ID: 101233144 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-7568 (Electronic) Linking ISSN: 17427061 NLM ISO Abbreviation: Acta Biomater Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Kidlington, Oxford, UK : Elsevier, c2004-
مواضيع طبية MeSH: Anti-Infective Agents/*pharmacology , Hydrogels/*pharmacology , Mesenchymal Stem Cells/*cytology , Minocycline/*pharmacology , Wound Healing/*drug effects, Adult ; Bacterial Adhesion/drug effects ; Cell Adhesion/drug effects ; Cell Survival/drug effects ; Human Umbilical Vein Endothelial Cells/drug effects ; Humans ; Immunomodulation/drug effects ; Intercellular Signaling Peptides and Proteins/pharmacology ; Mesenchymal Stem Cells/drug effects ; Microbial Sensitivity Tests ; Neovascularization, Physiologic/drug effects ; Phenotype ; Staphylococcus aureus/drug effects ; Staphylococcus aureus/growth & development
مستخلص: Mesenchymal stromal/stem cells (MSCs) have demonstrated pro-healing properties including an anti-inflammatory cytokine profile and the promotion of angiogenesis via expression of growth factors in pre-clinical models. MSCs encapsulated in poly(ethylene glycol) diacrylate (PEGdA) and thiolated gelatin poly(ethylene glycol) (Gel-PEG-Cys) crosslinked hydrogels have led to controlled cellular presentation at wound sites with favorable wound healing outcomes. However, the therapeutic potential of MSC-loaded hydrogels may be limited by non-specific protein adsorption on the delivery matrix that could facilitate the initial adhesion of microorganisms and subsequent virulent biofilm formation. Antimicrobials loaded concurrently in the hydrogels with MSCs could reduce microbial bioburden and promote healing, but the antimicrobial effect on the MSC wound healing capacity and the antibacterial efficacy of the hydrogels is unknown. We demonstrate that minocycline specifically induces a favorable change in MSC migration capacity, proliferation, gene expression, extracellular matrix (ECM) attachment, and adhesion molecule and growth factor release with subsequent increased angiogenesis. We then demonstrate that hydrogels loaded with MSCs, minocycline, vancomycin, and linezolid can significantly decrease bacterial bioburden. Our study suggests that minocycline can serve as a dual mechanism for the regenerative capacity of MSCs and the reduction of bioburden in triple antimicrobial-loaded hydrogels.
Statement of Significance: Wound healing is a complex biological process that can be hindered by bacterial infection, excessive inflammation, and inadequate microvasculature. In this study, we develop a new formulation of poly(ethylene glycol) diacrylate and thiolated gelatin poly(ethylene glycol) crosslinked hydrogels loaded with minocycline, vancomycin, linezolid, and mesenchymal stromal/stem cells that induces a favorable wound healing phenotype in mesenchymal stromal/stem cells and prevents bacterial bioburden on the hydrogel. This combinatorial approach to biomaterial development has the potential to impact wound healing for contaminated full thickness cutaneous wounds.
(Copyright © 2017. Published by Elsevier Ltd.)
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معلومات مُعتمدة: P30 CA014520 United States CA NCI NIH HHS; R21 HL115482 United States HL NHLBI NIH HHS; T32 GM008349 United States GM NIGMS NIH HHS
فهرسة مساهمة: Keywords: Hydrogel; Mesenchymal stromal/stem cells; Minocycline; Staphylococcus aureus; Wound healing
المشرفين على المادة: 0 (Anti-Infective Agents)
0 (Hydrogels)
0 (Intercellular Signaling Peptides and Proteins)
FYY3R43WGO (Minocycline)
تواريخ الأحداث: Date Created: 20170111 Date Completed: 20171106 Latest Revision: 20240610
رمز التحديث: 20240610
مُعرف محوري في PubMed: PMC5704963
DOI: 10.1016/j.actbio.2017.01.021
PMID: 28069512
قاعدة البيانات: MEDLINE
الوصف
تدمد:1878-7568
DOI:10.1016/j.actbio.2017.01.021