دورية أكاديمية
Virion Structure of Black Queen Cell Virus, a Common Honeybee Pathogen.
| العنوان: | Virion Structure of Black Queen Cell Virus, a Common Honeybee Pathogen. |
|---|---|
| المؤلفون: | Spurny R; Structural Virology, Central European Institute of Technology, Masaryk University, Brno, Czech Republic., Přidal A; Department of Zoology, Fishery, Hydrobiology, and Apidology, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic., Pálková L; Structural Virology, Central European Institute of Technology, Masaryk University, Brno, Czech Republic., Kiem HKT; Structural Virology, Central European Institute of Technology, Masaryk University, Brno, Czech Republic., de Miranda JR; Department of Ecology, Swedish University of Agricultural Sciences, Uppsala, Sweden., Plevka P; Structural Virology, Central European Institute of Technology, Masaryk University, Brno, Czech Republic pavel.plevka@ceitec.muni.cz. |
| المصدر: | Journal of virology [J Virol] 2017 Feb 28; Vol. 91 (6). Date of Electronic Publication: 2017 Feb 28 (Print Publication: 2017). |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society For Microbiology Country of Publication: United States NLM ID: 0113724 Publication Model: Electronic-Print Cited Medium: Internet ISSN: 1098-5514 (Electronic) Linking ISSN: 0022538X NLM ISO Abbreviation: J Virol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Washington Dc : American Society For Microbiology Original Publication: Baltimore, American Society for Microbiology. |
| مواضيع طبية MeSH: | Dicistroviridae/*ultrastructure , Virion/*ultrastructure, Animals ; Bees/virology ; Capsid Proteins/chemistry ; Crystallography, X-Ray ; Models, Molecular ; Protein Conformation ; Viral Structures |
| مستخلص: | Viral diseases are a major threat to honeybee ( Apis mellifera ) populations worldwide and therefore an important factor in reliable crop pollination and food security. Black queen cell virus (BQCV) is the etiological agent of a fatal disease of honeybee queen larvae and pupae. The virus belongs to the genus Triatovirus from the family Dicistroviridae , which is part of the order Picornavirales Here we present a crystal structure of BQCV determined to a resolution of 3.4 Å. The virion is formed by 60 copies of each of the major capsid proteins VP1, VP2, and VP3; however, there is no density corresponding to a 75-residue-long minor capsid protein VP4 encoded by the BQCV genome. We show that the VP4 subunits are present in the crystallized virions that are infectious. This aspect of the BQCV virion is similar to that of the previously characterized triatoma virus and supports the recent establishment of the separate genus Triatovirus within the family Dicistroviridae The C terminus of VP1 and CD loops of capsid proteins VP1 and VP3 of BQCV form 34-Å-tall finger-like protrusions at the virion surface. The protrusions are larger than those of related dicistroviruses. IMPORTANCE The western honeybee is the most important pollinator of all, and it is required to sustain the agricultural production and biodiversity of wild flowering plants. However, honeybee populations worldwide are suffering from virus infections that cause colony losses. One of the most common, and least known, honeybee pathogens is black queen cell virus (BQCV), which at high titers causes queen larvae and pupae to turn black and die. Here we present the three-dimensional virion structure of BQCV, determined by X-ray crystallography. The structure of BQCV reveals large protrusions on the virion surface. Capsid protein VP1 of BQCV does not contain a hydrophobic pocket. Therefore, the BQCV virion structure provides evidence that capsid-binding antiviral compounds that can prevent the replication of vertebrate picornaviruses may be ineffective against honeybee virus infections. (Copyright © 2017 Spurny et al.) |
| References: | Virology. 2004 Oct 10;328(1):151-7. (PMID: 15380366) J Gen Virol. 2004 Aug;85(Pt 8):2263-70. (PMID: 15269367) J Biol Chem. 1994 May 6;269(18):13680-4. (PMID: 8175803) Curr Biol. 1994 Sep 1;4(9):784-97. (PMID: 7820548) Virology. 2000 Nov 25;277(2):457-63. (PMID: 11080493) Nat Protoc. 2007;2(11):2728-33. (PMID: 18007608) Curr Top Microbiol Immunol. 2010;343:43-89. (PMID: 20397067) Appl Environ Microbiol. 2006 Apr;72(4):2414-20. (PMID: 16597939) Virology. 1998 Apr 25;244(1):50-8. (PMID: 9581777) PLoS Pathog. 2013 Mar;9(3):e1003240. (PMID: 23555253) Nature. 1985 Sep 12-18;317(6033):145-53. (PMID: 2993920) J Virol. 1990 May;64(5):1934-45. (PMID: 2157861) Arch Virol. 1999;144(10):2051-8. (PMID: 10550677) Mol Cell Biol. 2000 Jul;20(14):4990-9. (PMID: 10866656) Nucleic Acids Res. 2003 Jul 1;31(13):3497-500. (PMID: 12824352) Virology. 2011 Jan 5;409(1):91-101. (PMID: 21030058) Microbiol Rev. 1981 Jun;45(2):287-315. (PMID: 7022155) J Comput Chem. 2004 Oct;25(13):1605-12. (PMID: 15264254) Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):235-42. (PMID: 21460441) Acta Crystallogr A. 1991 Mar 1;47 ( Pt 2):110-9. (PMID: 2025413) J Mol Biol. 1996 Aug 9;261(1):1-10. (PMID: 8760498) Appl Environ Microbiol. 2004 Dec;70(12):7185-91. (PMID: 15574916) Structure. 1997 Dec 15;5(12):1557-69. (PMID: 9438862) Nucleic Acids Res. 2007 Jul;35(Web Server issue):W375-83. (PMID: 17452350) Antiviral Res. 2002 Feb;53(2):83-98. (PMID: 11750935) Virus Genes. 2013 Apr;46(2):362-8. (PMID: 23239276) Arch Virol. 2002 May;147(5):1071-6. (PMID: 12021875) J Virol. 2014 Mar;88(6):3114-26. (PMID: 24352461) Vet Microbiol. 2009 Nov 18;139(3-4):227-34. (PMID: 19570624) Appl Environ Microbiol. 2006 Jan;72(1):606-11. (PMID: 16391097) Acta Crystallogr D Biol Crystallogr. 1999 Apr;55(Pt 4):941-4. (PMID: 10089342) Insects. 2013 Jan 04;4(1):71-89. (PMID: 26466796) Nat Struct Mol Biol. 2012 Mar 04;19(4):424-9. (PMID: 22388738) J Gen Virol. 1998 Jan;79 ( Pt 1):191-203. (PMID: 9460942) Science. 2006 Jul 21;313(5785):351-4. (PMID: 16857940) Structure. 1996 Aug 15;4(8):897-904. (PMID: 8805582) Ecol Evol. 2013 Feb;3(2):298-311. (PMID: 23467539) J Gen Virol. 2000 Apr;81(Pt 4):1149-54. (PMID: 10725445) J Mol Graph. 1996 Feb;14(1):33-8, 27-8. (PMID: 8744570) J Virol. 1982 Dec;44(3):900-6. (PMID: 6294338) Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):14730-5. (PMID: 10611281) Virology. 2006 Jun 20;350(1):67-78. (PMID: 16574186) J Gen Virol. 2000 Aug;81(Pt 8):2111-9. (PMID: 10900051) Virology. 1991 Feb;180(2):781-7. (PMID: 1846498) J Virol. 2011 Jan;85(2):776-83. (PMID: 20980499) J Gen Virol. 2002 Dec;83(Pt 12):3131-8. (PMID: 12466490) Nat Struct Biol. 1999 Aug;6(8):765-74. (PMID: 10426956) Virology. 1984 Jun;135(2):546-50. (PMID: 6204448) PLoS Pathog. 2014 Aug 21;10(8):e1004323. (PMID: 25144447) Science. 1986 Sep 19;233(4770):1286-93. (PMID: 3018924) Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32. (PMID: 15572765) J Invertebr Pathol. 2010 Jan;103 Suppl 1:S80-95. (PMID: 19909973) Methods Enzymol. 1997;276:594-611. (PMID: 9048382) Bioinformatics. 1998;14(1):68-73. (PMID: 9520503) Arch Virol. 2008;153(4):715-27. (PMID: 18293057) Nature. 2015 Jan 1;517(7532):85-8. (PMID: 25327248) J Virol. 2015 Nov 18;90(3):1377-86. (PMID: 26581987) Environ Microbiol. 2015 Apr;17(4):969-83. (PMID: 25611325) J Virol. 2016 Aug 26;90(18):8150-9. (PMID: 27384649) Acta Crystallogr D Biol Crystallogr. 2013 Jun;69(Pt 6):1026-37. (PMID: 23695247) J Gen Virol. 2002 Apr;83(Pt 4):915-26. (PMID: 11907342) Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):125-32. (PMID: 20124692) J Gen Virol. 2007 Dec;88(Pt 12):3428-38. (PMID: 18024913) Acta Crystallogr D Biol Crystallogr. 1995 Jul 1;51(Pt 4):473-89. (PMID: 15299834) Trends Ecol Evol. 2010 Jun;25(6):345-53. (PMID: 20188434) J Anim Ecol. 2015 May;84(3):615-24. (PMID: 25646973) Virology. 1998 Mar 30;243(1):54-65. (PMID: 9527915) Nature. 1993 Jan 14;361(6408):176-9. (PMID: 8421524) Microbiol Mol Biol Rev. 2014 Sep;78(3):418-37. (PMID: 25184560) |
| فهرسة مساهمة: | Keywords: Apis mellifera; Cripavirus; Dicistroviridae; Picornavirales; Triatovirus; X ray; X-ray crystallography; capsid; crystallography; honey bee; honeybee; insect disease; structure; virion; virus |
| المشرفين على المادة: | 0 (Capsid Proteins) |
| تواريخ الأحداث: | Date Created: 20170113 Date Completed: 20170518 Latest Revision: 20190329 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC5331821 |
| DOI: | 10.1128/JVI.02100-16 |
| PMID: | 28077635 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1098-5514 |
|---|---|
| DOI: | 10.1128/JVI.02100-16 |