دورية أكاديمية
Post-translational selective intracellular silencing of acetylated proteins with de novo selected intrabodies.
| العنوان: | Post-translational selective intracellular silencing of acetylated proteins with de novo selected intrabodies. |
|---|---|
| المؤلفون: | Chirichella M; Bio@SNS Laboratory, Scuola Normale Superiore, Pisa, Italy., Lisi S; Bio@SNS Laboratory, Scuola Normale Superiore, Pisa, Italy., Fantini M; Bio@SNS Laboratory, Scuola Normale Superiore, Pisa, Italy., Goracci M; Bio@SNS Laboratory, Scuola Normale Superiore, Pisa, Italy., Calvello M; Bio@SNS Laboratory, Scuola Normale Superiore, Pisa, Italy., Brandi R; Genomic Facility, European Brain Research Institute (EBRI), Roma, Italy., Arisi I; Genomic Facility, European Brain Research Institute (EBRI), Roma, Italy., D'Onofrio M; Genomic Facility, European Brain Research Institute (EBRI), Roma, Italy., Di Primio C; Bio@SNS Laboratory, Scuola Normale Superiore, Pisa, Italy., Cattaneo A; Bio@SNS Laboratory, Scuola Normale Superiore, Pisa, Italy. |
| المصدر: | Nature methods [Nat Methods] 2017 Mar; Vol. 14 (3), pp. 279-282. Date of Electronic Publication: 2017 Jan 16. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Pub. Group Country of Publication: United States NLM ID: 101215604 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1548-7105 (Electronic) Linking ISSN: 15487091 NLM ISO Abbreviation: Nat Methods Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York, NY : Nature Pub. Group, c2004- |
| مواضيع طبية MeSH: | Antibodies/*immunology , HIV Integrase/*immunology , HIV Integrase/*metabolism , Histones/*immunology , Histones/*metabolism , Protein Processing, Post-Translational/*immunology, Acetylation ; Humans |
| مستخلص: | The ability to selectively interfere with post-translationally modified proteins would have many biological and therapeutic applications. However, post-translational modifications cannot be selectively targeted by nucleic-acid-based interference approaches. Here we describe post-translational intracellular silencing antibody technology (PISA), a method for selecting intrabodies against post-translationally modified proteins. We demonstrate our method by generating intrabodies against native acetylated proteins and showing functional interference in living cells. |
| المشرفين على المادة: | 0 (Antibodies) 0 (Histones) EC 2.7.7.- (HIV Integrase) YY6481J2FF (p31 integrase protein, Human immunodeficiency virus 1) |
| تواريخ الأحداث: | Date Created: 20170117 Date Completed: 20170626 Latest Revision: 20181202 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1038/nmeth.4144 |
| PMID: | 28092690 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1548-7105 |
|---|---|
| DOI: | 10.1038/nmeth.4144 |