دورية أكاديمية
أعرض في EDS

Identification of Novel Macropinocytosing Human Antibodies by Phage Display and High-Content Analysis.

التفاصيل البيبلوغرافية
العنوان: Identification of Novel Macropinocytosing Human Antibodies by Phage Display and High-Content Analysis.
المؤلفون: Ha KD; UCSF Helen Diller Family Comprehensive Cancer Center, University of California at San Francisco, San Francisco, CA, United States., Bidlingmaier SM; UCSF Helen Diller Family Comprehensive Cancer Center, University of California at San Francisco, San Francisco, CA, United States., Su Y; UCSF Helen Diller Family Comprehensive Cancer Center, University of California at San Francisco, San Francisco, CA, United States., Lee NK; UCSF Helen Diller Family Comprehensive Cancer Center, University of California at San Francisco, San Francisco, CA, United States., Liu B; UCSF Helen Diller Family Comprehensive Cancer Center, University of California at San Francisco, San Francisco, CA, United States. Electronic address: bin.liu@ucsf.edu.
المصدر: Methods in enzymology [Methods Enzymol] 2017; Vol. 585, pp. 91-110. Date of Electronic Publication: 2016 Dec 01.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural
اللغة: English
بيانات الدورية: Publisher: Academic Press Country of Publication: United States NLM ID: 0212271 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1557-7988 (Electronic) Linking ISSN: 00766879 NLM ISO Abbreviation: Methods Enzymol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: New York, Academic Press.
مواضيع طبية MeSH: Antibody Affinity/*physiology , Neoplasms/*immunology, Antibodies/immunology ; Antibody Specificity ; Antigens, Neoplasm/immunology ; Endocytosis/physiology ; Humans ; Peptide Library
مستخلص: Internalizing antibodies have great potential for the development of targeted therapeutics. Antibodies that internalize via the macropinocytosis pathway are particularly promising since macropinocytosis is capable of mediating rapid, bulk uptake and is selectively upregulated in many cancers. We hereby describe a method for identifying antibodies that internalize via macropinocytosis by screening phage-displayed single-chain antibody selection outputs with an automated fluorescent microscopy-based high-content analysis platform. Furthermore, this method can be similarly applied to other endocytic pathways if other fluorescent, pathway-specific, soluble markers are available.
(© 2017 Elsevier Inc. All rights reserved.)
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معلومات مُعتمدة: R01 CA118919 United States CA NCI NIH HHS; R01 CA129491 United States CA NCI NIH HHS; R01 CA171315 United States CA NCI NIH HHS
فهرسة مساهمة: Keywords: Cancer cell membrane dynamics; Cancer metabolism; Endocytosis pathway; High-content analysis; High-throughput screening; Human monoclonal antibodies; Macropinocytosis; Phage antibody display
المشرفين على المادة: 0 (Antibodies)
0 (Antigens, Neoplasm)
0 (Peptide Library)
تواريخ الأحداث: Date Created: 20170123 Date Completed: 20170905 Latest Revision: 20211216
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC8671048
DOI: 10.1016/bs.mie.2016.10.004
PMID: 28109445
قاعدة البيانات: MEDLINE
الوصف
تدمد:1557-7988
DOI:10.1016/bs.mie.2016.10.004