دورية أكاديمية
Heterologous expression and characterization of plant Taxadiene-5α-Hydroxylase (CYP725A4) in Escherichia coli.
العنوان: | Heterologous expression and characterization of plant Taxadiene-5α-Hydroxylase (CYP725A4) in Escherichia coli. |
---|---|
المؤلفون: | Rouck JE; Department of Comparative Biosciences, Department of Biochemistry, Department of Bioengineering, Division of Nutritional Science, Center for Biophysics and Quantitative Biology, Beckman Institute for Advanced Science and Technology, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA., Biggs BW; Manus Biosynthesis, 1030 Massachusetts Avenue, Suite 300, Cambridge, MA 02138, USA; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL 60208, USA., Kambalyal A; Department of Comparative Biosciences, Department of Biochemistry, Department of Bioengineering, Division of Nutritional Science, Center for Biophysics and Quantitative Biology, Beckman Institute for Advanced Science and Technology, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA., Arnold WR; Department of Comparative Biosciences, Department of Biochemistry, Department of Bioengineering, Division of Nutritional Science, Center for Biophysics and Quantitative Biology, Beckman Institute for Advanced Science and Technology, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA., De Mey M; Centre for Industrial Biotechnology and Biocatalysis, Ghent University, Coupure Links 653, B-9000, Belgium., Ajikumar PK; Manus Biosynthesis, 1030 Massachusetts Avenue, Suite 300, Cambridge, MA 02138, USA. Electronic address: pkaji@manusbio.com., Das A; Department of Comparative Biosciences, Department of Biochemistry, Department of Bioengineering, Division of Nutritional Science, Center for Biophysics and Quantitative Biology, Beckman Institute for Advanced Science and Technology, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA. Electronic address: aditidas@illinois.edu. |
المصدر: | Protein expression and purification [Protein Expr Purif] 2017 Apr; Vol. 132, pp. 60-67. Date of Electronic Publication: 2017 Jan 18. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 9101496 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0279 (Electronic) Linking ISSN: 10465928 NLM ISO Abbreviation: Protein Expr Purif Subsets: MEDLINE |
أسماء مطبوعة: | Publication: Orlando, FL : Academic Press Original Publication: San Diego : Academic Press, c1990- |
مواضيع طبية MeSH: | Cytochrome P-450 Enzyme System*/biosynthesis , Cytochrome P-450 Enzyme System*/chemistry , Cytochrome P-450 Enzyme System*/genetics , Cytochrome P-450 Enzyme System*/isolation & purification , Plant Proteins*/biosynthesis , Plant Proteins*/chemistry , Plant Proteins*/genetics , Plant Proteins*/isolation & purification, Alkenes/*chemistry , Diterpenes/*chemistry , Escherichia coli/*metabolism , Taxus/*genetics, Binding Sites ; Escherichia coli/genetics ; Kinetics ; Recombinant Proteins/biosynthesis ; Recombinant Proteins/chemistry ; Recombinant Proteins/genetics ; Recombinant Proteins/isolation & purification ; Taxus/enzymology |
مستخلص: | Taxadiene-5α-Hydroxylase (CYP725A4) is a membrane-bound plant cytochrome P450 that catalyzes the oxidation of taxadiene to taxadiene-5α-ol. This oxidation is a key step in the production of the valuable cancer therapeutic and natural plant product, taxol. In this work, we report the bacterial expression and purification of six different constructs of CYP725A4. All six of these constructs are N-terminally modified and three of them are fused to cytochrome P450 reductase to form a chimera construct. The construct with the highest yield of CYP725A4 protein was then selected for substrate binding and kinetic analysis. Taxadiene binding followed type-1 substrate patterns with an observed K (Copyright © 2017 Elsevier Inc. All rights reserved.) |
References: | Proc Natl Acad Sci U S A. 2002 May 14;99(10):6725-30. (PMID: 11997441) Protein Eng. 2003 Aug;16(8):577-87. (PMID: 12968075) Biotechniques. 2003 Sep;35(3):556-60, 562-3. (PMID: 14513561) Protein Sci. 2003 Nov;12(11):2476-81. (PMID: 14573860) Arch Biochem Biophys. 2004 Apr 15;424(2):141-53. (PMID: 15047186) Chem Biol. 2004 Mar;11(3):379-87. (PMID: 15123267) Proc Natl Acad Sci U S A. 1991 Oct 15;88(20):9141-5. (PMID: 1656462) Biotechniques. 2006 May;40(5):601-2, 604, 606, passim. (PMID: 16708760) J Struct Funct Genomics. 2006 Mar;7(1):1-14. (PMID: 16850178) Proc Natl Acad Sci U S A. 2006 Aug 1;103(31):11509-14. (PMID: 16864771) Biochemistry. 2007 Feb 27;46(8):2059-69. (PMID: 17263563) Bioinformatics. 2007 Nov 1;23(21):2947-8. (PMID: 17846036) J Am Chem Soc. 2007 Nov 14;129(45):13778-9. (PMID: 17948999) J Biol Chem. 2008 Mar 7;283(10):6067-75. (PMID: 18167342) Proc Natl Acad Sci U S A. 1991 Jul 1;88(13):5597-601. (PMID: 1829523) PLoS Genet. 2008 Nov;4(11):e1000286. (PMID: 19043575) Tetrahedron. 2008;64(27):6561-6567. (PMID: 19122848) FEBS Lett. 2010 May 3;584(9):1721-7. (PMID: 19836392) Biochemistry. 2009 Dec 29;48(51):12104-12. (PMID: 19908820) Biochim Biophys Acta. 2011 Jan;1814(1):223-9. (PMID: 20685623) Science. 2010 Oct 1;330(6000):70-4. (PMID: 20929806) Science. 2010 Dec 3;330(6009):1355-8. (PMID: 21127247) Pak J Biol Sci. 2011 Apr 15;14(8):466-75. (PMID: 21936250) Annu Rev Chem Biomol Eng. 2011;2:211-36. (PMID: 22432617) J Biotechnol. 2013 Jan 20;163(2):166-78. (PMID: 22687248) Curr Opin Biotechnol. 2013 Apr;24(2):291-9. (PMID: 22985679) Nature. 2012 Dec 13;492(7428):188-9. (PMID: 23235869) Protein Sci. 2013 Jul;22(7):964-79. (PMID: 23661295) Protein Expr Purif. 2013 Nov;92(1):77-87. (PMID: 23973802) Appl Environ Microbiol. 2014 Nov;80(21):6828-36. (PMID: 25172862) Nat Protoc. 2015 Jun;10(6):845-58. (PMID: 25950237) Biochim Biophys Acta. 2015 Oct;1848(10 Pt A):2460-2470. (PMID: 26232558) ACS Chem Biol. 2016 Feb 19;11(2):460-9. (PMID: 26677870) ACS Chem Biol. 2016 May 20;11(5):1445-51. (PMID: 26930136) Proc Natl Acad Sci U S A. 2016 Mar 22;113(12):3209-14. (PMID: 26951651) Chem Sci. 2016 May 1;7(5):3102-3107. (PMID: 29997802) Biochem Pharmacol. 1978 Mar 1;27(5):783-8. (PMID: 656117) Arch Biochem Biophys. 1995 Oct 20;323(1):87-96. (PMID: 7487078) Annu Rev Pharmacol Toxicol. 1995;35:369-90. (PMID: 7598499) Anticancer Res. 1995 Jan-Feb;15(1):93-8. (PMID: 7733649) Arch Biochem Biophys. 1994 Aug 1;312(2):436-46. (PMID: 8037457) J Mol Graph. 1996 Feb;14(1):33-8, 27-8. (PMID: 8744570) Chem Biol. 1996 Jun;3(6):479-89. (PMID: 8807878) Arch Biochem Biophys. 1997 Mar 1;339(1):107-14. (PMID: 9056240) Proc Natl Acad Sci U S A. 1997 Aug 5;94(16):8411-6. (PMID: 9237990) |
معلومات مُعتمدة: | R01 GM115584 United States GM NIGMS NIH HHS |
فهرسة مساهمة: | Keywords: E. coli; Nanodiscs; Recombinant expression; Taxadiene; Taxadiene-5α-ol |
المشرفين على المادة: | 0 (Alkenes) 0 (Diterpenes) 0 (Plant Proteins) 0 (Recombinant Proteins) 0 (taxa-4(5),11(12)diene) 9035-51-2 (Cytochrome P-450 Enzyme System) |
تواريخ الأحداث: | Date Created: 20170123 Date Completed: 20170703 Latest Revision: 20201209 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC5741078 |
DOI: | 10.1016/j.pep.2017.01.008 |
PMID: | 28109855 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1096-0279 |
---|---|
DOI: | 10.1016/j.pep.2017.01.008 |