دورية أكاديمية
Molecular Mechanisms of Colistin Resistance in Klebsiella pneumoniae Causing Bacteremia from India-A First Report.
| العنوان: | Molecular Mechanisms of Colistin Resistance in Klebsiella pneumoniae Causing Bacteremia from India-A First Report. |
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| المؤلفون: | Pragasam AK; Department of Clinical Microbiology, Christian Medical College Vellore, India., Shankar C; Department of Clinical Microbiology, Christian Medical College Vellore, India., Veeraraghavan B; Department of Clinical Microbiology, Christian Medical College Vellore, India., Biswas I; Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Centre Kansas, KS, USA., Nabarro LE; Department of Clinical Microbiology, Christian Medical College Vellore, India., Inbanathan FY; Department of Clinical Microbiology, Christian Medical College Vellore, India., George B; Department of Haematology, Christian Medical College Vellore, India., Verghese S; Department of Nephrology, Christian Medical College Vellore, India. |
| المصدر: | Frontiers in microbiology [Front Microbiol] 2017 Jan 09; Vol. 7, pp. 2135. Date of Electronic Publication: 2017 Jan 09 (Print Publication: 2016). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101548977 Publication Model: eCollection Cited Medium: Print ISSN: 1664-302X (Print) Linking ISSN: 1664302X NLM ISO Abbreviation: Front Microbiol Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne : Frontiers Research Foundation |
| مستخلص: | Colistin has long been a reserve drug used for the treatment of carbapenem resistant Klebsiella pneumoniae . Carbapenem resistance in K. pneumoniae has been increasing and is as high as 44% in India. Although a reserve agent, with rise in rates of resistance to carbapenems, the usage of colistin has increased over the years leading to slow emergence of resistance. Colistin resistance is mainly mediated by the alteration in the LPS of bacterial outer membrane with the addition of L-Ara4-N and PEtN molecules. These alterations are mediated by mutations in several genes involved in lipidA modifications and most commonly mutations in mgrB gene has been reported. Recently there is emergence of plasmid mediated resistance due to mcr-1 and mcr-2 genes which poses a threat for the rapid global spread. This study aims at characterizing eight colistin resistant K. pneumoniae from bacteremia by whole genome sequencing. Eight K. pneumoniae were isolated from blood culture during 2013 and 2014 at the Department of Clinical Microbiology, Christian Medical College, India. Antimicrobial susceptibility testing was performed and minimum inhibitory concentration (MIC) was determined for colistin and polymyxin B by broth-micro dilution method. Whole genome sequencing was performed using Ion Torrent and the genome of all eight isolates was analyzed. The eight isolates were resistant to all the antimicrobials expect tigecycline. MIC of colistin and polymyxin B were ranged from 4 to 1024 μg/ml and 0.5 to 2048 μg/ml respectively. Multiple mutations were observed in the chromosomal genes involved in lipid A modifications. mcr-1 and mcr-2 gene was absent in all the isolates. The most significant were mutations in mgrB gene. Among the eight isolates, four, three and one were belonged to sequence types ST 231, ST14 and ST147 respectively. Seven isolates had bla |
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| فهرسة مساهمة: | Keywords: Klebsiella pneumoniae; colistin; polymyxin B; resistance; whole genome sequencing |
| تواريخ الأحداث: | Date Created: 20170126 Latest Revision: 20220318 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC5220082 |
| DOI: | 10.3389/fmicb.2016.02135 |
| PMID: | 28119670 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1664-302X |
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| DOI: | 10.3389/fmicb.2016.02135 |