دورية أكاديمية
أعرض في EDS

Transport capabilities of environmental Pseudomonads for sulfur compounds.

التفاصيل البيبلوغرافية
العنوان: Transport capabilities of environmental Pseudomonads for sulfur compounds.
المؤلفون: Zerbs S; Biosciences Division, Argonne National Laboratory, 9700 S Cass Ave, Lemont, Illinois, 60439., Korajczyk PJ; Biosciences Division, Argonne National Laboratory, 9700 S Cass Ave, Lemont, Illinois, 60439., Noirot PH; Biosciences Division, Argonne National Laboratory, 9700 S Cass Ave, Lemont, Illinois, 60439., Collart FR; Biosciences Division, Argonne National Laboratory, 9700 S Cass Ave, Lemont, Illinois, 60439.
المصدر: Protein science : a publication of the Protein Society [Protein Sci] 2017 Apr; Vol. 26 (4), pp. 784-795. Date of Electronic Publication: 2017 Feb 10.
نوع المنشور: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: Cold Spring Harbor Laboratory Press Country of Publication: United States NLM ID: 9211750 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1469-896X (Electronic) Linking ISSN: 09618368 NLM ISO Abbreviation: Protein Sci Subsets: MEDLINE
أسماء مطبوعة: Publication: 2001- : Woodbury, NY : Cold Spring Harbor Laboratory Press
Original Publication: New York, N.Y. : Cambridge University Press, c1992-
مواضيع طبية MeSH: ATP-Binding Cassette Transporters/*metabolism , Bacterial Proteins/*metabolism , Pseudomonas/*metabolism , Sulfur Compounds/*metabolism, ATP-Binding Cassette Transporters/genetics ; Bacterial Proteins/genetics ; Biological Transport, Active/physiology ; Pseudomonas/genetics
مستخلص: Sulfur is an essential element in plant rhizospheres and microbial activity plays a key role in increasing the biological availability of sulfur in soil environments. To better understand the mechanisms facilitating the exchange of sulfur-containing molecules in soil, we profiled the binding specificities of eight previously uncharacterized ABC transporter solute-binding proteins from plant-associated Pseudomonads. A high-throughput screening procedure indicated eighteen significant organosulfur binding ligands, with at least one high-quality screening hit for each protein target. Calorimetric and spectroscopic methods were used to validate the best ligand assignments and catalog the thermodynamic properties of the protein-ligand interactions. Two novel high-affinity ligand-binding activities were identified and quantified in this set of solute-binding proteins. Bacteria were cultured in minimal media with screening library components supplied as the sole sulfur sources, demonstrating that these organosulfur compounds can be metabolized and confirming the relevance of ligand assignments. These results expand the set of experimentally validated ligands amenable to transport by this ABC transporter family and demonstrate the complex range of protein-ligand interactions that can be accomplished by solute-binding proteins. Characterizing new nutrient import pathways provides insight into Pseudomonad metabolic capabilities which can be used to further interrogate bacterial survival and participation in soil and rhizosphere communities.
(© 2017 The Protein Society.)
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فهرسة مساهمة: Keywords: ABC transporter; Pseudomonas fluorescens; Pseudomonas protegens; organosulfur compounds; rhizosphere
المشرفين على المادة: 0 (ATP-Binding Cassette Transporters)
0 (Bacterial Proteins)
0 (Sulfur Compounds)
تواريخ الأحداث: Date Created: 20170128 Date Completed: 20170717 Latest Revision: 20181113
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC5368066
DOI: 10.1002/pro.3124
PMID: 28127814
قاعدة البيانات: MEDLINE
الوصف
تدمد:1469-896X
DOI:10.1002/pro.3124