دورية أكاديمية
أعرض في EDS

MISTIC-fusion proteins as antigens for high quality membrane protein antibodies.

التفاصيل البيبلوغرافية
العنوان: MISTIC-fusion proteins as antigens for high quality membrane protein antibodies.
المؤلفون: Alves NS; Friedrich Miescher Laboratory of the Max Planck Society, Spemannstraße 39, 72076 Tübingen, Germany., Astrinidis SA; Friedrich Miescher Laboratory of the Max Planck Society, Spemannstraße 39, 72076 Tübingen, Germany., Eisenhardt N; Friedrich Miescher Laboratory of the Max Planck Society, Spemannstraße 39, 72076 Tübingen, Germany., Sieverding C; Friedrich Miescher Laboratory of the Max Planck Society, Spemannstraße 39, 72076 Tübingen, Germany., Redolfi J; Friedrich Miescher Laboratory of the Max Planck Society, Spemannstraße 39, 72076 Tübingen, Germany., Lorenz M; Friedrich Miescher Laboratory of the Max Planck Society, Spemannstraße 39, 72076 Tübingen, Germany., Weberruss M; Friedrich Miescher Laboratory of the Max Planck Society, Spemannstraße 39, 72076 Tübingen, Germany., Moreno-Andrés D; Friedrich Miescher Laboratory of the Max Planck Society, Spemannstraße 39, 72076 Tübingen, Germany., Antonin W; Friedrich Miescher Laboratory of the Max Planck Society, Spemannstraße 39, 72076 Tübingen, Germany.
المصدر: Scientific reports [Sci Rep] 2017 Feb 02; Vol. 7, pp. 41519. Date of Electronic Publication: 2017 Feb 02.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Antibodies/*metabolism , Antigens/*metabolism , Membrane Proteins/*metabolism , Recombinant Fusion Proteins/*metabolism, Animals ; Calnexin/metabolism ; Chromatography, Affinity ; Immune Sera/metabolism ; Nuclear Envelope/metabolism ; Solubility ; Xenopus Proteins/metabolism ; Xenopus laevis
مستخلص: Lack of high-quality antibodies against transmembrane proteins is a widely recognized hindrance in biomedical and cell biological research. Here we present a robust pipeline for the generation of polyclonal antibodies employing full-length membrane proteins as immunogens to overcome this "antibody bottleneck". We express transmembrane proteins fused to a MISTIC fragment that enhances expression of eukaryotic membrane proteins in E. coli. Purified membrane proteins are used as immunogen for rabbit injection employing standard immunizing protocols. The raised antibodies against membrane proteins of the endoplasmic reticulum and the nuclear envelope, which we use as test cases, function in a wide range of applications and are superior to ones produced against soluble domains as immunogens.
Competing Interests: The authors declare no competing financial interests.
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المشرفين على المادة: 0 (Antibodies)
0 (Antigens)
0 (Immune Sera)
0 (Membrane Proteins)
0 (Recombinant Fusion Proteins)
0 (Xenopus Proteins)
139873-08-8 (Calnexin)
تواريخ الأحداث: Date Created: 20170203 Date Completed: 20181024 Latest Revision: 20181113
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC5288655
DOI: 10.1038/srep41519
PMID: 28148968
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/srep41519