دورية أكاديمية
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A class C radical S-adenosylmethionine methyltransferase synthesizes 8-methylmenaquinone.

التفاصيل البيبلوغرافية
العنوان: A class C radical S-adenosylmethionine methyltransferase synthesizes 8-methylmenaquinone.
المؤلفون: Hein S; Microbial Energy Conversion and Biotechnology, Department of Biology, Technische Universität Darmstadt, Schnittspahnstraße 10, 64287, Darmstadt, Germany., Klimmek O; Microbial Energy Conversion and Biotechnology, Department of Biology, Technische Universität Darmstadt, Schnittspahnstraße 10, 64287, Darmstadt, Germany., Polly M; Microbial Energy Conversion and Biotechnology, Department of Biology, Technische Universität Darmstadt, Schnittspahnstraße 10, 64287, Darmstadt, Germany., Kern M; Microbial Energy Conversion and Biotechnology, Department of Biology, Technische Universität Darmstadt, Schnittspahnstraße 10, 64287, Darmstadt, Germany., Simon J; Microbial Energy Conversion and Biotechnology, Department of Biology, Technische Universität Darmstadt, Schnittspahnstraße 10, 64287, Darmstadt, Germany.
المصدر: Molecular microbiology [Mol Microbiol] 2017 May; Vol. 104 (3), pp. 449-462. Date of Electronic Publication: 2017 Feb 28.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Blackwell Scientific Publications Country of Publication: England NLM ID: 8712028 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2958 (Electronic) Linking ISSN: 0950382X NLM ISO Abbreviation: Mol Microbiol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Oxford, OX ; Boston, MA : Blackwell Scientific Publications, c1987-
مواضيع طبية MeSH: Methyltransferases/*metabolism , S-Adenosylmethionine/*metabolism , Vitamin K 2/*metabolism , Wolinella/*metabolism, Bacterial Proteins/metabolism ; Escherichia coli/enzymology ; Escherichia coli/metabolism ; Humans ; Oxidation-Reduction ; Wolinella/enzymology
مستخلص: The membranous quinone/quinol pool is essential to the majority of life forms and has been widely used as an important biomarker in microbial taxonomy. In the anaerobic world, the most important quinones are menaquinone (MK) and a methylated form of MK, designated methylmenaquinone (MMK), which is anticipated to serve specifically in low-potential electron transport chains involved in anaerobic respiration. However, it has remained unclear how MMK is generated. Here, we show that a novel enzyme homologous to class C radical SAM methyltransferases (RSMTs) synthesizes MMK using MK as substrate. Such enzymes, termed either MenK or MqnK, are present in MMK-producing bacteria (and some archaea) that possess either the classical MK biosynthesis pathway (Men) or the futalosine pathway (Mqn). An mqnK deletion mutant of the model Epsilonproteobacterium Wolinella succinogenes was unable to produce MMK 6 but its formation was restored upon genomic complementation using either the native mqnK gene or menK from the human gut bacterium Adlercreutzia equolifaciens or Shewanella oneidensis. Moreover, any of the menK genes enabled Escherichia coli cells to produce MMK 8 and a methylated form of 2-demethylmenaquinone 8 (DMK 8 ). The results expand the knowledge on quinone synthesis and demonstrate an unprecedented function for a class C RSMT-type enzyme in primary cell metabolism.
(© 2017 John Wiley & Sons Ltd.)
المشرفين على المادة: 0 (Bacterial Proteins)
11032-49-8 (Vitamin K 2)
7LP2MPO46S (S-Adenosylmethionine)
EC 2.1.1.- (Methyltransferases)
تواريخ الأحداث: Date Created: 20170207 Date Completed: 20170530 Latest Revision: 20170530
رمز التحديث: 20240628
DOI: 10.1111/mmi.13638
PMID: 28164386
قاعدة البيانات: MEDLINE
الوصف
تدمد:1365-2958
DOI:10.1111/mmi.13638