دورية أكاديمية
Rapid Molecular Detection of Zika Virus in Acute-Phase Urine Samples Using the Recombinase Polymerase Amplification Assay.
| العنوان: | Rapid Molecular Detection of Zika Virus in Acute-Phase Urine Samples Using the Recombinase Polymerase Amplification Assay. |
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| المؤلفون: | Abd El Wahed A; Division of Microbiology and Animal Hygiene, Institute of Veterinary Medicine, Department of Animal Sciences, Georg-August-University, Goettingen, Lower Saxony, Germany., Sanabani SS; Hospital das Clínicas, School of Medicine, University of São Paulo, São Paulo, Brazil., Faye O; Arbovirus and viral hemorragic fever unit, Institut Pasteur de Dakar, Dakar, Senegal., Pessôa R; Hospital das Clínicas, School of Medicine, University of São Paulo, São Paulo, Brazil., Patriota JV; Municipal Hospital of Tuparetama, Tuparetama, Pernambuco, Brazil., Giorgi RR; University of Santo Amaro, São Paulo, São Paulo, Brazil., Patel P; TIB MOLBIOL Syntheselabor GmbH, Berlin, Germany., Böhlken-Fascher S; Georg-August-University Goettingen., Landt O; TIB MOLBIOL Syntheselabor GmbH, Berlin, Germany., Niedrig M; Robert Koch Institut Robert Koch Institut., Zanotto PM; Laboratory of Molecular Evolution and Bioinformatics, Department of Microbiology, Biomedical Sciences Institute, University of São Paulo, São Paulo, Brazil., Czerny CP; Division of Microbiology and Animal Hygiene, Institute of Veterinary Medicine, Department of Animal Sciences, Georg-August-University, Goettingen, Lower Saxony, Germany., Sall AA; Arbovirus and viral hemorragic fever unit, Institut Pasteur de Dakar, Dakar, Senegal., Weidmann M |
| المصدر: | PLoS currents [PLoS Curr] 2017 Jan 25; Vol. 9. Date of Electronic Publication: 2017 Jan 25. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Public Library of Science Country of Publication: United States NLM ID: 101515638 Publication Model: Electronic Cited Medium: Internet ISSN: 2157-3999 (Electronic) Linking ISSN: 21573999 NLM ISO Abbreviation: PLoS Curr Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: San Francisco, CA : Public Library of Science, 2009-2018 |
| مستخلص: | Background: Currently the detection of Zika virus (ZIKV) in patient samples is done by real-time RT-PCR. Samples collected from rural area are sent to highly equipped laboratories for screening. A rapid point-of-care test is needed to detect the virus, especially at low resource settings. Methodology/principal Findings: In this report, we describe the development of a reverse transcription isothermal recombinase polymerase amplification (RT-RPA) assay for the identification of ZIKV. RT-RPA assay was portable, sensitive (21 RNA molecules), and rapid (3-15 minutes). No cross-reactivity was detected to other flaviviruses, alphaviruses and arboviruses. Compared to real-time RT-PCR, the diagnostic sensitivity was 92%, while the specificity was 100%. Conclusions/significance: The developed assay is a promising platform for rapid point of need detection of ZIKV in low resource settings and elsewhere (e.g. during mass gathering). |
| References: | Arq Neuropsiquiatr. 2016 Mar;74(3):253-5. (PMID: 27050856) J Clin Microbiol. 2013 Apr;51(4):1110-7. (PMID: 23345286) Medicine (Baltimore). 2016 Mar;95(12):e3201. (PMID: 27015222) PLoS Negl Trop Dis. 2016 Sep 29;10(9):e0004953. (PMID: 27685649) J Clin Virol. 2015 Aug;69:16-21. (PMID: 26209370) PLoS One. 2015 Jun 15;10(6):e0129682. (PMID: 26075598) Lancet Infect Dis. 2016 Jun;16(6):653-60. (PMID: 26897108) Euro Surveill. 2015;20(44):null. (PMID: 26558690) |
| فهرسة مساهمة: | Keywords: diagnostics; point of need; zika virus |
| تواريخ الأحداث: | Date Created: 20170228 Latest Revision: 20200930 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC5309125 |
| DOI: | 10.1371/currents.outbreaks.a7f1db2c7d66c3fc0ea0a774305d319e |
| PMID: | 28239513 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2157-3999 |
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| DOI: | 10.1371/currents.outbreaks.a7f1db2c7d66c3fc0ea0a774305d319e |