دورية أكاديمية
أعرض في EDS

Differential role of pannexin-1/ATP/P2X 7 axis in IL-1β release by human monocytes.

التفاصيل البيبلوغرافية
العنوان: Differential role of pannexin-1/ATP/P2X 7 axis in IL-1β release by human monocytes.
المؤلفون: Parzych K; Department of Cardiovascular Pharmacology, Vascular Biology, National Heart and Lung Institute, Imperial College London, London, United Kingdom., Zetterqvist AV; Department of Clinical Sciences, Lund University, Malmö, Sweden., Wright WR; Department of Cardiovascular Pharmacology, Vascular Biology, National Heart and Lung Institute, Imperial College London, London, United Kingdom., Kirkby NS; Department of Cardiovascular Pharmacology, Vascular Biology, National Heart and Lung Institute, Imperial College London, London, United Kingdom., Mitchell JA; Department of Cardiovascular Pharmacology, Vascular Biology, National Heart and Lung Institute, Imperial College London, London, United Kingdom., Paul-Clark MJ; Department of Cardiovascular Pharmacology, Vascular Biology, National Heart and Lung Institute, Imperial College London, London, United Kingdom; m.paul-clark@imperial.ac.uk.
المصدر: FASEB journal : official publication of the Federation of American Societies for Experimental Biology [FASEB J] 2017 Jun; Vol. 31 (6), pp. 2439-2445. Date of Electronic Publication: 2017 Feb 28.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Federation of American Societies for Experimental Biology Country of Publication: United States NLM ID: 8804484 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1530-6860 (Electronic) Linking ISSN: 08926638 NLM ISO Abbreviation: FASEB J Subsets: MEDLINE
أسماء مطبوعة: Publication: 2020- : [Bethesda, Md.] : Hoboken, NJ : Federation of American Societies for Experimental Biology ; Wiley
Original Publication: [Bethesda, Md.] : The Federation, [c1987-
مواضيع طبية MeSH: Adenosine Triphosphate/*metabolism , Connexins/*metabolism , Interleukin-1beta/*metabolism , Monocytes/*metabolism , Nerve Tissue Proteins/*metabolism , Receptors, Purinergic P2X7/*metabolism, Adenosine Triphosphate/genetics ; Caspase 1/genetics ; Caspase 1/metabolism ; Cell Line, Tumor ; Connexins/genetics ; Diglycerides/pharmacology ; Gene Expression Regulation/physiology ; Humans ; Interleukin-1beta/genetics ; Lipopeptides/pharmacology ; Lipopolysaccharides/pharmacology ; Nerve Tissue Proteins/genetics ; Oligopeptides/pharmacology ; Receptors, Purinergic P2X7/genetics ; Toll-Like Receptor 2/agonists ; Toll-Like Receptor 2/genetics ; Toll-Like Receptor 2/metabolism ; Toll-Like Receptor 4/agonists ; Toll-Like Receptor 4/genetics ; Toll-Like Receptor 4/metabolism
مستخلص: IL-1β release is integral to the innate immune system. The release of mature IL-1β depends on 2 regulated events: the de novo induction of pro-IL-1β, generally via NF-κB-dependent transduction pathways; and the assembly and activation of the NLRP3 inflammasome. This latter step is reliant on active caspase-1, pannexin-1, and P2X 7 receptor activation. Pathogen-associated molecular patterns in gram-positive and gram-negative bacteria activate IL-1β release from immune cells via TLR2 and TLR4 receptors, respectively. We found that pro-IL-1β and mature IL-1β release from human monocytes is stimulated by the TLR2 agonists Pam 3 CSK4 or FSL-1, as well as the TLR4 agonist LPS in the absence of additional ATP. TLR2 agonists required pannexin-1 and P2X 7 receptor activation to stimulate IL-1β release. In contrast, IL-1β release stimulated by the TLR4 agonist LPS is independent of both pannexin-1 and P2X 7 activation. In the absence of exogenous ATP, P2X 7 activation requires endogenous ATP release, which occurs in some cells via pannexin-1. In line with this, we found that LPS-stimulated human monocytes released relatively low levels of ATP, whereas cells stimulated with TLR2 agonists released high levels of ATP. These findings suggest that in human monocytes, both TLR2 and TLR4 signaling induce pro-IL-1β expression, but the mechanism by which they activate caspase-1 diverges at the level of the pannexin-1/ATP/P2X 7 axis.-Parzych, K., Zetterqvist, A. V., Wright, W. R., Kirkby, N. S., Mitchell, J. A., Paul-Clark, M. J. Differential role of pannexin-1/ATP/P2X 7 axis in IL-1β release by human monocytes.
(© FASEB.)
References: Epilepsy Curr. 2012 Mar;12(2):66-8. (PMID: 22473546)
Proc Natl Acad Sci U S A. 2010 Nov 9;107(45):19449-54. (PMID: 20974980)
FEBS Lett. 2006 Jan 9;580(1):239-44. (PMID: 16364313)
J Biol Chem. 2002 Feb 1;277(5):3210-8. (PMID: 11706016)
J Endotoxin Res. 2001;7(3):243-7. (PMID: 11581578)
Br J Pharmacol. 2006 Dec;149(7):880-7. (PMID: 17031385)
Infect Immun. 2006 Jul;74(7):4354-6. (PMID: 16790810)
Nature. 2006 Mar 9;440(7081):237-41. (PMID: 16407889)
Semin Immunol. 2013 Dec 15;25(6):469-84. (PMID: 24275598)
EMBO J. 2006 Nov 1;25(21):5071-82. (PMID: 17036048)
Am J Physiol Cell Physiol. 2004 May;286(5):C1100-8. (PMID: 15075209)
Am J Physiol Cell Physiol. 2008 Sep;295(3):C752-60. (PMID: 18596211)
J Endotoxin Res. 2003;9(3):181-6. (PMID: 12831460)
Curr Opin Immunol. 2007 Dec;19(6):615-22. (PMID: 17977705)
J Exp Med. 1997 Feb 3;185(3):579-82. (PMID: 9053458)
Mol Cell. 2002 Aug;10(2):417-26. (PMID: 12191486)
Blood. 2009 Mar 5;113(10 ):2324-35. (PMID: 19104081)
J Leukoc Biol. 2009 Nov;86(5):1227-38. (PMID: 19675207)
J Biol Chem. 2007 Jan 26;282(4):2386-94. (PMID: 17121814)
معلومات مُعتمدة: United Kingdom Wellcome Trust; FS/16/1/31699 United Kingdom BHF_ British Heart Foundation
فهرسة مساهمة: Keywords: Toll-like receptors; inflammasome; innate immunity; potassium channels
المشرفين على المادة: 0 (Connexins)
0 (Diglycerides)
0 (FSL-1 lipoprotein, synthetic)
0 (Interleukin-1beta)
0 (Lipopeptides)
0 (Lipopolysaccharides)
0 (Nerve Tissue Proteins)
0 (Oligopeptides)
0 (PANX1 protein, human)
0 (Pam(3)CSK(4) peptide)
0 (Receptors, Purinergic P2X7)
0 (TLR2 protein, human)
0 (TLR4 protein, human)
0 (Toll-Like Receptor 2)
0 (Toll-Like Receptor 4)
8L70Q75FXE (Adenosine Triphosphate)
EC 3.4.22.36 (Caspase 1)
تواريخ الأحداث: Date Created: 20170302 Date Completed: 20170912 Latest Revision: 20220129
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC5507675
DOI: 10.1096/fj.201600256
PMID: 28246166
قاعدة البيانات: MEDLINE
الوصف
تدمد:1530-6860
DOI:10.1096/fj.201600256