دورية أكاديمية
أعرض في EDS

Gene expression profiles in preterm infants on continuous long‑term oxygen therapy suggest reduced oxidative stress‑dependent signaling during hypoxia.

التفاصيل البيبلوغرافية
العنوان: Gene expression profiles in preterm infants on continuous long‑term oxygen therapy suggest reduced oxidative stress‑dependent signaling during hypoxia.
المؤلفون: Kalikstad B; University of Oslo, Institute of Clinical Medicine, Women and Children's Clinic, Rikshospitalet, 0372 Oslo, Norway., Kultima HG; Department of Medical Sciences, Science for Life Laboratory, Uppsala University, 751 23 Uppsala, Sweden., Andersstuen TK; Department of Molecular Biology, Institute of Medical Microbiology, Oslo University Hospital, Rikshospitalet, 0372 Oslo, Norway., Klungland A; Department of Molecular Biology, Institute of Medical Microbiology, Oslo University Hospital, Rikshospitalet, 0372 Oslo, Norway., Isaksson A; Department of Medical Sciences, Science for Life Laboratory, Uppsala University, 751 23 Uppsala, Sweden.
المصدر: Molecular medicine reports [Mol Med Rep] 2017 Apr; Vol. 15 (4), pp. 1513-1526. Date of Electronic Publication: 2017 Feb 08.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: D. A. Spandidos Country of Publication: Greece NLM ID: 101475259 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1791-3004 (Electronic) Linking ISSN: 17912997 NLM ISO Abbreviation: Mol Med Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Athens, Greece : D. A. Spandidos
مواضيع طبية MeSH: Gene Expression Profiling*, Hypoxia/*genetics , Infant, Premature/*metabolism , Oxidative Stress/*genetics , Oxygen/*therapeutic use , Signal Transduction/*genetics, Antioxidant Response Elements/genetics ; Cohort Studies ; Female ; Gene Expression Regulation ; Humans ; Hypoxia-Inducible Factor 1, alpha Subunit/metabolism ; Infant ; Infant, Newborn ; Male ; Molecular Sequence Annotation ; NF-E2-Related Factor 2/metabolism ; Time Factors ; Transcriptome
مستخلص: Preterm infants are susceptible to neonatal inflammatory/infective diseases requiring drug therapy. The present study hypothesized that mRNA expression in the blood may be modulated by signaling pathways during treatment. The current study aimed to explore changes in global gene expression in the blood from preterm infants with the objective of identifying patterns or pathways of potential relevance to drug therapy. The infants involved were selected based on maternal criteria indicating increased risk for therapeutic intervention. Global mRNA expression was measured in 107 longitudinal whole blood samples using Affymetrix Human‑Genome‑U133 Plus 2.0‑arrays; samples were obtained from 20 preterm infants. Unsupervised clustering revealed a distinct homogeneous gene expression pattern in 13 samples derived from seven infants undergoing continuous oxygen therapy. At these sampling times, all but one of the seven infants exhibited severe drops in peripheral capillary saturation levels below 60%. The infants were reoxygenated with 100% inspired oxygen concentration. The other samples (n=94) represented the infants from the cohort at time points when they did not undergo continuous oxygen therapy. Comparing these two sets of samples identified a distinct gene expression pattern of 5,986 significantly differentially expressed genes, of which 5,167 genes exhibited reduced expression levels during transient hypoxia. This expression pattern was reversed when the infants became stable, i.e., when they were not continuously oxygenated and had no events of hypoxia. To identify signaling pathways involved in gene regulation, the Database for Annotation, Visualization and Integrated Discovery online tool was used. Mitogen‑activated protein kinases, which are normally induced by oxidative stress, exhibited reduced gene expression during hypoxia. In addition, nuclear factor erythroid 2‑related factor 2‑antioxidant response element target genes involved in oxidative stress protection were also expressed at lower levels, suggesting reduced transcription of this pathway. The findings of the present study suggest that oxidative stress‑dependent signaling is reduced during hypoxia. Understanding the molecular response in preterm infants during continuous oxygenation may aid in refining therapeutic strategies for oxygen therapy.
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المشرفين على المادة: 0 (HIF1A protein, human)
0 (Hypoxia-Inducible Factor 1, alpha Subunit)
0 (NF-E2-Related Factor 2)
0 (NFE2L2 protein, human)
S88TT14065 (Oxygen)
تواريخ الأحداث: Date Created: 20170306 Date Completed: 20170619 Latest Revision: 20181113
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC5364962
DOI: 10.3892/mmr.2017.6185
PMID: 28259955
قاعدة البيانات: MEDLINE
الوصف
تدمد:1791-3004
DOI:10.3892/mmr.2017.6185