دورية أكاديمية
Size-dependent inhibition of herpesvirus cellular entry by polyvalent nanoarchitectures.
| العنوان: | Size-dependent inhibition of herpesvirus cellular entry by polyvalent nanoarchitectures. |
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| المؤلفون: | Ziem B; Institute of Chemistry and Biochemistry, Freie Universität Berlin, 14195 Berlin, Germany. haag@chemie.fu-berlin.de., Azab W; Institut für Virologie, Robert von Ostertag-Haus, Zentrum für Infektionsmedizin, Freie Universität Berlin, Robert-von-Ostertag-Str. 7-13, 14163 Berlin, Germany. no.34@fu-berlin.de., Gholami MF; Department of Physics & IRIS Adlershof, Humboldt-Universität Berlin, D-12489 Berlin, Germany., Rabe JP; Department of Physics & IRIS Adlershof, Humboldt-Universität Berlin, D-12489 Berlin, Germany., Osterrieder N; Institut für Virologie, Robert von Ostertag-Haus, Zentrum für Infektionsmedizin, Freie Universität Berlin, Robert-von-Ostertag-Str. 7-13, 14163 Berlin, Germany. no.34@fu-berlin.de., Haag R; Institute of Chemistry and Biochemistry, Freie Universität Berlin, 14195 Berlin, Germany. haag@chemie.fu-berlin.de. |
| المصدر: | Nanoscale [Nanoscale] 2017 Mar 17; Vol. 9 (11), pp. 3774-3783. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: RSC Pub Country of Publication: England NLM ID: 101525249 Publication Model: Print Cited Medium: Internet ISSN: 2040-3372 (Electronic) Linking ISSN: 20403364 NLM ISO Abbreviation: Nanoscale Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cambridge, UK : RSC Pub. |
| مواضيع طبية MeSH: | Graphite* , Nanostructures* , Virus Internalization*, Herpesvirus 1, Equid/*physiology , Herpesvirus 1, Human/*physiology, Animals ; Cells, Cultured ; Chlorocebus aethiops ; Horses ; Polymers ; Skin/cytology ; Vero Cells |
| مستخلص: | Carbon-based architectures, especially graphene and its derivatives, have recently attracted much attention in the field of biomedicine and biotechnology for their use as pathogen inhibitors or biosensors. One of the major problems in the development of novel virus inhibitor systems is the adaption of the inhibitor to the size of virus particles. We here report the synthesis and biological testing of carbon-based inhibitors differing in size for evaluating the potential size effect on the inhibition of virus entry and replication. In this context, different sized nanomaterials were functionalized with polygylcerol through a "grafting from" polymerization to form new polyvalent nanoarchitectures which can operate as viral inhibitor systems after post-modification. For this purpose a polysulfation was carried out to mimic the heparan sulfates present on cell surfaces that we reasoned would compete with the binding sites of herpes simplex virus type 1 (HSV-1) and equine herpesvirus type 1 (EHV-1), which both cause major global health issues. Our results clearly demonstrate that the inhibitory efficiency is regulated by the size of the polymeric nanomaterials and the degree of sulfation. The best inhibiting graphene sheets were ∼300 nm in size and had a degree of sulfation of ∼10%. Furthermore, it turned out that the derivatives inhibited virus infection at an early stage during entry but did not affect cell-to-cell spread. Overall, tunable polyvalent nanomaterials are promising and efficient virus entry inhibitors, which can likely be used for a broad spectrum of enveloped viruses. |
| المشرفين على المادة: | 0 (Polymers) 7782-42-5 (Graphite) |
| تواريخ الأحداث: | Date Created: 20170308 Date Completed: 20181102 Latest Revision: 20191210 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1039/c7nr00611j |
| PMID: | 28266670 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2040-3372 |
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| DOI: | 10.1039/c7nr00611j |