دورية أكاديمية
Monascus ruber-Fermented Buckwheat (Red Yeast Buckwheat) Suppresses Adipogenesis in 3T3-L1 Cells.
| العنوان: | Monascus ruber-Fermented Buckwheat (Red Yeast Buckwheat) Suppresses Adipogenesis in 3T3-L1 Cells. |
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| المؤلفون: | Hong H; 1 Department of Medical Science, Konkuk University School of Medicine , Seoul, Korea., Park J; 2 Department of Animal Life and Environmental Science, College of Agriculture and Life Science, Hankyong National University , Anseong, Korea., Lumbera WL; 2 Department of Animal Life and Environmental Science, College of Agriculture and Life Science, Hankyong National University , Anseong, Korea., Hwang SG; 2 Department of Animal Life and Environmental Science, College of Agriculture and Life Science, Hankyong National University , Anseong, Korea. |
| المصدر: | Journal of medicinal food [J Med Food] 2017 Apr; Vol. 20 (4), pp. 352-359. Date of Electronic Publication: 2017 Mar 23. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 9812512 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1557-7600 (Electronic) Linking ISSN: 1096620X NLM ISO Abbreviation: J Med Food Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Larchmont, NY : Mary Ann Liebert, Inc., c1998- |
| مواضيع طبية MeSH: | Fermentation*, Adipocytes/*drug effects , Adipogenesis/*drug effects , Anti-Obesity Agents/*chemistry , Eriogonum/*chemistry , Monascus/*metabolism, 3T3-L1 Cells ; Animals ; Cell Cycle Checkpoints ; Cell Differentiation ; Fatty Acid Synthases/chemistry ; Fatty Acid-Binding Proteins/chemistry ; Gene Expression ; Leptin/chemistry ; Lipids/chemistry ; Mice ; Obesity/drug therapy ; Transcription Factors/chemistry |
| مستخلص: | Although various treatments have been used for weight loss to date, obese people rarely have safe and effective treatment options. Therefore, the antiobesity effects of several natural compounds are being actively investigated. This study was conducted to investigate the antiadipogenic effects of Monascus ruber-fermented Fagopyrum esculentum (red yeast buckwheat, RYB) in 3T3-L1 cells. We assessed the intracellular lipid content and adipocyte differentiation by oil red O staining and the expression of genes and proteins associated with adipocyte differentiation by reverse transcription-polymerase chain reaction and western blotting in 3T3-L1 cells. RYB dose dependently inhibited 3T3-L1 cell differentiation at concentrations of 50-800 μg/mL, without cytotoxic effects. It also suppressed the expression of adipogenic transcription factors, including peroxisome proliferator-activated receptor γ and CCAAT/enhancer-binding protein α, and adipocyte-specific genes, such as adipocyte fatty acid-binding protein (aP2), fatty acid synthase, and leptin, during preadipocyte differentiation into adipocytes. Furthermore, RYB reduced cyclin-dependent kinase 2 and cyclin expression and increased p21 and p27 expression, thus causing cell cycle arrest at the G1/S phase. Collectively, these results suggest that RYB may be an effective nutraceutical for weight loss as indicated by its ability to suppress adipogenesis-specific gene expression and cause cell cycle arrest at the G1/S interphase. |
| فهرسة مساهمة: | Keywords: C/EBPα; FAS; Monascus ruber; PPARγ; aP2; adipogenesis; fermented buckwheat; leptin; obesity |
| المشرفين على المادة: | 0 (Anti-Obesity Agents) 0 (Fatty Acid-Binding Proteins) 0 (Leptin) 0 (Lipids) 0 (Transcription Factors) EC 2.3.1.85 (Fatty Acid Synthases) |
| تواريخ الأحداث: | Date Created: 20170324 Date Completed: 20170515 Latest Revision: 20170515 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1089/jmf.2016.3761 |
| PMID: | 28332893 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1557-7600 |
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| DOI: | 10.1089/jmf.2016.3761 |