دورية أكاديمية
أعرض في EDS

Development of fusogenic glass surfaces that impart spatiotemporal control over macrophage fusion: Direct visualization of multinucleated giant cell formation.

التفاصيل البيبلوغرافية
العنوان: Development of fusogenic glass surfaces that impart spatiotemporal control over macrophage fusion: Direct visualization of multinucleated giant cell formation.
المؤلفون: Faust JJ; School of Life Sciences, Arizona State University, Tempe, AZ 85287, USA; Center for Metabolic and Vascular Biology, Mayo Clinic, Scottsdale, AZ 85259, USA., Christenson W; Department of Physics, Arizona State University, Tempe, AZ 85287, USA; Center for Biological Physics, Arizona State University, Tempe, AZ 85287, USA., Doudrick K; Department of Civil and Environmental Engineering and Earth Sciences, University of Notre Dame, Notre Dame, IN 46556, USA., Ros R; Department of Physics, Arizona State University, Tempe, AZ 85287, USA; Center for Biological Physics, Arizona State University, Tempe, AZ 85287, USA., Ugarova TP; School of Life Sciences, Arizona State University, Tempe, AZ 85287, USA; Center for Metabolic and Vascular Biology, Mayo Clinic, Scottsdale, AZ 85259, USA. Electronic address: Tatiana.Ugarova@asu.edu.
المصدر: Biomaterials [Biomaterials] 2017 Jun; Vol. 128, pp. 160-171. Date of Electronic Publication: 2017 Mar 02.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 8100316 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-5905 (Electronic) Linking ISSN: 01429612 NLM ISO Abbreviation: Biomaterials Subsets: MEDLINE
أسماء مطبوعة: Publication: <1995-> : Amsterdam : Elsevier Science
Original Publication: [Guilford, England] : IPC Science and Technology Press, 1980-
مواضيع طبية MeSH: Giant Cells/*cytology , Glass/*chemistry , Macrophages/*cytology, Adsorption ; Animals ; Cell Adhesion/drug effects ; Cell Fusion ; Giant Cells/drug effects ; Giant Cells/metabolism ; HEK293 Cells ; Humans ; Macrophage-1 Antigen/metabolism ; Macrophages/drug effects ; Macrophages/metabolism ; Mice, Inbred C57BL ; Oleic Acids/pharmacology ; Optical Phenomena ; Surface Properties ; Time Factors
مستخلص: Implantation of synthetic material, including vascular grafts, pacemakers, etc. results in the foreign body reaction and the formation of multinucleated giant cells (MGCs) at the exterior surface of the implant. Despite the long-standing premise that fusion of mononucleated macrophages results in the formation of MGCs, to date, no published study has shown fusion in context with living specimens. This is due to the fact that optical-quality glass, which is required for the majority of live imaging techniques, does not promote macrophage fusion. Consequently, the morphological changes that macrophages undergo during fusion as well as the mechanisms that govern this process remain ill-defined. In this study, we serendipitously identified a highly fusogenic glass surface and discovered that the capacity to promote fusion was due to oleamide contamination. When adsorbed on glass, oleamide and other molecules that contain long-chain hydrocarbons promoted high levels of macrophage fusion. Adhesion, an essential step for macrophage fusion, was apparently mediated by Mac-1 integrin (CD11b/CD18, α M β 2 ) as determined by single cell force spectroscopy and adhesion assays. Micropatterned glass further increased fusion and enabled a remarkable degree of spatiotemporal control over MGC formation. Using these surfaces, we reveal the kinetics that govern MGC formation in vitro. We anticipate that the spatiotemporal control afforded by these surfaces will expedite studies designed to identify the mechanism(s) of macrophage fusion and MGC formation with implication for the design of novel biomaterials.
(Copyright © 2017 Elsevier Ltd. All rights reserved.)
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معلومات مُعتمدة: R01 HL063199 United States HL NHLBI NIH HHS
فهرسة مساهمة: Keywords: Foreign body reaction; Live imaging; Mac-1 integrin; Macrophage fusion; Microscopy; Multinucleated giant cells
المشرفين على المادة: 0 (Macrophage-1 Antigen)
0 (Oleic Acids)
7L25QK8BWO (oleylamide)
تواريخ الأحداث: Date Created: 20170325 Date Completed: 20171218 Latest Revision: 20181113
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC5540443
DOI: 10.1016/j.biomaterials.2017.02.031
PMID: 28340410
قاعدة البيانات: MEDLINE
الوصف
تدمد:1878-5905
DOI:10.1016/j.biomaterials.2017.02.031