دورية أكاديمية
The RegA regulon exhibits variability in response to altered growth conditions and differs markedly between Rhodobacter species.
| العنوان: | The RegA regulon exhibits variability in response to altered growth conditions and differs markedly between Rhodobacter species. |
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| المؤلفون: | Schindel HS; Biochemistry, Indiana University Bloomington, Simon Hall MSB, 212 S. Hawthorne Dr., Bloomington, IN 47405-7003, USA., Bauer CE; Biochemistry, Indiana University Bloomington, Simon Hall MSB, 212 S. Hawthorne Dr., Bloomington, IN 47405-7003, USA. |
| المصدر: | Microbial genomics [Microb Genom] 2016 Oct 21; Vol. 2 (10), pp. e000081. Date of Electronic Publication: 2016 Oct 21 (Print Publication: 2016). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Microbiology Society Country of Publication: England NLM ID: 101671820 Publication Model: eCollection Cited Medium: Print ISSN: 2057-5858 (Print) Linking ISSN: 20575858 NLM ISO Abbreviation: Microb Genom Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oct. 2015- : [London] : Microbiology Society Original Publication: [London] : Society for General Microbiology, [2015]- |
| مواضيع طبية MeSH: | Gene Expression Regulation, Bacterial/*genetics , Regulon/*genetics , Rhodobacter/*genetics, Bacterial Proteins/genetics ; Rhodobacter capsulatus ; Rhodobacter sphaeroides ; Species Specificity |
| مستخلص: | The RegB/RegA two-component system from Rhodobacter capsulatus regulates global changes in gene expression in response to alterations in oxygen levels. Studies have shown that RegB/RegA controls many energy-generating and energy-utilizing systems such as photosynthesis, nitrogen fixation, carbon fixation, hydrogen utilization, respiration, electron transport and denitrification. In this report, we utilized RNA-seq and ChIP-seq to analyse the breadth of genes indirectly and directly regulated by RegA. A comparison of mRNA transcript levels in wild type cells relative to a RegA deletion strain shows that there are 257 differentially expressed genes under photosynthetic defined minimal growth medium conditions and 591 differentially expressed genes when grown photosynthetically in a complex rich medium. ChIP-seq analysis also identified 61 unique RegA binding sites with a well-conserved recognition sequence, 33 of which exhibit changes in neighbouring gene expression. These transcriptome results define new members of the RegA regulon including genes involved in iron transport and motility. These results also reveal that the set of genes that are regulated by RegA are growth medium specific. Similar analyses under dark aerobic conditions where RegA is thought not to be phosphorylated by RegB reveal 40 genes that are differentially expressed in minimal medium and 20 in rich medium. Finally, a comparison of the R. capsulatus RegA regulon with the orthologous PrrA regulon in Rhodobacter sphaeroides shows that the number of photosystem genes regulated by RegA and PrrA are similar but that the identity of genes regulated by RegA and PrrA beyond those involved in photosynthesis are quite distinct. |
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| معلومات مُعتمدة: | R01 GM040941 United States GM NIGMS NIH HHS; R37 GM040941 United States GM NIGMS NIH HHS |
| فهرسة مساهمة: | Keywords: PrrA homologue; global transcription factor binding; microbial photosynthesis; redox regulation |
| المشرفين على المادة: | 0 (Bacterial Proteins) |
| تواريخ الأحداث: | Date Created: 20170329 Date Completed: 20181101 Latest Revision: 20240324 |
| رمز التحديث: | 20240324 |
| مُعرف محوري في PubMed: | PMC5359404 |
| DOI: | 10.1099/mgen.0.000081 |
| PMID: | 28348828 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2057-5858 |
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| DOI: | 10.1099/mgen.0.000081 |