دورية أكاديمية
أعرض في EDS

Lack of Association between Angiotensin Converting Enzyme I/D Polymorphism and Unexplained Recurrent Miscarriage in Saudi Arabia.

التفاصيل البيبلوغرافية
العنوان: Lack of Association between Angiotensin Converting Enzyme I/D Polymorphism and Unexplained Recurrent Miscarriage in Saudi Arabia.
المؤلفون: Al-Mukaynizi FB; Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia., AlKhuriji A; Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia., Babay Z; Department of Obs/Gyn, College of Medicine, King Saud University, Riyadh, Saudi Arabia., Addar M; Department of Obs/Gyn, College of Medicine, King Saud University, Riyadh, Saudi Arabia., AlDaihan S; Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia., Alanazi M; Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia., Warsy AS; Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
المصدر: Journal of medical biochemistry [J Med Biochem] 2016 Apr; Vol. 35 (2), pp. 166-173. Date of Electronic Publication: 2016 May 09.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Society of Medical Biochemists of Serbia Country of Publication: Serbia NLM ID: 101315490 Publication Model: Print-Electronic Cited Medium: Print ISSN: 1452-8258 (Print) Linking ISSN: 14528266 NLM ISO Abbreviation: J Med Biochem Subsets: PubMed not MEDLINE
أسماء مطبوعة: Publication: 2020- : Belgrade : Society of Medical Biochemists of Serbia
Original Publication: Belgrade : Society of Medical Biochemists of Serbia
مستخلص: Background: An insertion/deletion (I/D) polymorphism in the angiotensin converting enzyme (ACE) gene has been associated with recurrent miscarriage (RM) in several populations. We initiated this study to determine the association, if any, between the I/D polymorphism of ACE gene and RM in Saudi females.
Method: This study was conducted on 61 Saudi females suffering from RM (mean age: 34.1±6.2 years; range 15-45) attending clinics at King Khalid University Hospital, and 59 age matched females who had at least 2 children, as controls. Blood samples were drawn in EDTA tubes by venipuncture. DNA was extracted using the Puregene DNA purification kits. Insertion/Deletion (I/D) polymorphism of ACE gene was investigated by amplifying the genomic DNA by PCR using gene-specific primers. A single 190 bp or 490 bp band was obtained in the homozygous cases for the D allele or I allele, respectively, while the presence of both 190 and 490 bp bands indicated heterozygosity (ID).
Statistical Analysis: Deviation from Hardy-Weinberg equilibrium was determined (http://ihg.gsf.de/cgi-bin/hw/hwa1.pl). A standard chi-square (χ 2 ) test was used for comparing the genotype and allele frequencies in the two groups and Students't' test and χ 2 test were employed to compare values between the two groups. P<0.05 was considered statistically significant.
Results: The frequencies of DD, ID, and II genotypes were 56.7%, 29.5% and 4.9%, respectively, in females with RM and 54.2%, 42.3% and 3.3% respectively in the control group, but the difference was not statistically significant.
Conclusion: In some populations, meta-analyses showed an association between I/D polymorphism and RM risk, and the D allele was implicated as an increased risk factor for RM. However, this association was not apparent in the Saudi females.
Competing Interests: Authors declare that they do not have any competing interests with any group.
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فهرسة مساهمة: Keywords: ACE polymorphism; habitual abortion; recurrent miscarriage; recurrent pregnancy loss; renin–angiotensin system
Local Abstract: [Publisher, Serbian] Insercioni/delecijski polimorfizam (I/D) u genu za AKE (angiotenzin-konvertujući enzim) doveden je u vezu sa višestrukim spontanim pobačajima (VSP) u nekoliko populacija. Ovu studiju smo sproveli kako bismo utvrdili da li postoji ili ne postoji povezanost između I/D polimorfizma gena za AKE i VSP kod žena u Saudijskoj Arabiji. [Publisher, Serbian] Studija je obuhvatila 65 žena saudijske nacionalnosti sa VSP (prosek godina: 34,1±6,2 godina; raspon 15–45) koje su se lečile na klinikama Univerzitetske bolnice kralj Halid i 65 žena iste starosne dobi koje su imale najmanje dvoje dece, kao kontrolnu grupu. Uzorci krvi sakupljani su u EDTA epruvete venepunkcijom. DNK je ekstrahovana pomoću Puregene DNA purification kitova. Insercioni/delecijski (I/D) polimorfizam gena za AKE je ispitivan putem amplifikacije genomske DNK pomoću PCR uz korišćenje prajmera specifičnih za gene. U slučaju homozigota za D alel ili I alel dobijana je po jedna traka duga 190 bp ili 490 bp, dok je prisustvo obe trake od 190 i 490 bp značilo heterozigotnost (ID). [Publisher, Serbian] Procenjeno je odstupanje od Hardi-Vajnbergove ravnoteže. Za poređenje učestalosti genotipova i alela u dve grupe korišćen je standardni hi-kvadratni test (c2), dok su za poređenje vrednosti između dve grupe primenjeni studentov T test i c2 test. P < 0,05 smatrano je statistički značajnim. [Publisher, Serbian] Učestalosti DD, ID i II genotipova bile su 56,7%, 29,5% i 4,9% kod žena sa VSP, odnosno 54,2%, 42,3% i 3,3% u kontrolnoj grupi, ali razlika nije bila statistički značajna. [Publisher, Serbian] U nekim populacijama metaanalize su pokazale povezanost između I/D polimorfizma i rizika za VSP, ali D alel je bio umešan kao faktor povećanog rizika za VSP. Međutim, ova povezanost nije bila ispoljena kod saudijskih žena.
تواريخ الأحداث: Date Created: 20170331 Latest Revision: 20220408
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC5346794
DOI: 10.1515/jomb-2015-0020
PMID: 28356877
قاعدة البيانات: MEDLINE
الوصف
تدمد:1452-8258
DOI:10.1515/jomb-2015-0020