دورية أكاديمية
DynaMiTES - A dynamic cell culture platform for in vitro drug testing PART 2 - Ocular DynaMiTES for drug absorption studies of the anterior eye.
| العنوان: | DynaMiTES - A dynamic cell culture platform for in vitro drug testing PART 2 - Ocular DynaMiTES for drug absorption studies of the anterior eye. |
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| المؤلفون: | Beiβner N; Institut für Pharmazeutische Technologie, Technische Universität Braunschweig, Mendelssohnstraβe 1, 38106 Braunschweig, Germany; Center of Pharmaceutical Engineering - PVZ, Technische Universität Braunschweig, Franz-Liszt-Straβe 35 A, 38106 Braunschweig, Germany., Mattern K; Institut für Mikrotechnik, Technische Universität Braunschweig, Alte Salzdahlumer Straβe 203, 38124 Braunschweig, Germany; Center of Pharmaceutical Engineering - PVZ, Technische Universität Braunschweig, Franz-Liszt-Straβe 35 A, 38106 Braunschweig, Germany., Dietzel A; Institut für Mikrotechnik, Technische Universität Braunschweig, Alte Salzdahlumer Straβe 203, 38124 Braunschweig, Germany; Center of Pharmaceutical Engineering - PVZ, Technische Universität Braunschweig, Franz-Liszt-Straβe 35 A, 38106 Braunschweig, Germany., Reichl S; Institut für Pharmazeutische Technologie, Technische Universität Braunschweig, Mendelssohnstraβe 1, 38106 Braunschweig, Germany; Center of Pharmaceutical Engineering - PVZ, Technische Universität Braunschweig, Franz-Liszt-Straβe 35 A, 38106 Braunschweig, Germany. Electronic address: s.reichl@tu-braunschweig.de. |
| المصدر: | European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V [Eur J Pharm Biopharm] 2018 May; Vol. 126, pp. 166-176. Date of Electronic Publication: 2017 Apr 02. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 9109778 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3441 (Electronic) Linking ISSN: 09396411 NLM ISO Abbreviation: Eur J Pharm Biopharm Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Science Original Publication: Stuttgart : Wissenschaftliche Verlagsgesellschaft, c1991- |
| مواضيع طبية MeSH: | Cell Culture Techniques/*methods , Cell Membrane Permeability/*physiology , Cornea/*metabolism , Fluorescent Dyes/*metabolism , Tissue Engineering/*methods, Cell Line, Transformed ; Cell Membrane Permeability/drug effects ; Cell Survival/drug effects ; Cell Survival/physiology ; Cornea/drug effects ; Drug Evaluation, Preclinical/methods ; Equipment Design/methods ; Female ; Fluorescent Dyes/administration & dosage ; Humans ; Middle Aged ; Transendothelial and Transepithelial Migration/drug effects ; Transendothelial and Transepithelial Migration/physiology |
| مستخلص: | In the present study, a formerly designed Dynamic Micro Tissue Engineering System (DynaMiTES) was applied with our prevalidated human hemicornea (HC) construct to obtain a test platform for improved absorption studies of the anterior eye (Ocular DynaMiTES). First, the cultivation procedure of the classic HC was slightly adapted to the novel DynaMiTES design. The obtained inverted HC was then compared to classic HC regarding cell morphology using light and scanning electron microscopy, cell viability using MTT dye reaction and epithelial barrier properties observing transepithelial electrical resistance and apparent permeation coefficient of sodium fluorescein. These tested cell criteria were similar. In addition, the effects of four different flow rates on the same cell characteristics were investigated using the DynaMiTES. Because no harmful potential of flow was found, dynamic absorption studies of sodium fluorescein with and without 0.005%, 0.01% and 0.02% benzalkonium chloride were performed compared to the common static test procedure. In this proof-of-concept study, the dynamic test conditions showed different results than the static test conditions with a better prediction of in vivo data. Thus, we propose that our DynaMiTES platform provides great opportunities for the improvement of common in vitro drug testing procedures. (Copyright © 2017 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Benzalkonium chloride; DynaMiTES; Dynamic flow system; Human hemicornea construct; In vitro drug absorption; Microfluidic system; Organ on chip; TEER measurement |
| المشرفين على المادة: | 0 (Fluorescent Dyes) |
| تواريخ الأحداث: | Date Created: 20170406 Date Completed: 20180924 Latest Revision: 20180924 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.ejpb.2017.03.021 |
| PMID: | 28377274 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-3441 |
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| DOI: | 10.1016/j.ejpb.2017.03.021 |