دورية أكاديمية
Cellular Prion Protein PrP C and Ecto-5'-Nucleotidase Are Markers of the Cellular Stress Response to Aneuploidy.
| العنوان: | Cellular Prion Protein PrP C and Ecto-5'-Nucleotidase Are Markers of the Cellular Stress Response to Aneuploidy. |
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| المؤلفون: | Domingues PH; Miltenyi Biotec GmbH, Bergisch Gladbach, Germany., Nanduri LSY; Miltenyi Biotec GmbH, Bergisch Gladbach, Germany.; Amrita Centre for Nanosciences and Molecular Medicine, Amrita Vishwa Vidyapeetham, Kochi, Kerala, India., Seget K; Group Maintenance of Genome Stability, Max Planck Institute of Biochemistry, Martinsried, Germany.; Department of Molecular Genetics, University of Kaiserslautern, Kaiserslautern, Germany., Venkateswaran SV; Division of Molecular Thoracic Oncology, Deutsches Krebsforschungszentrum (DKFZ), Heidelberg, Germany., Agorku D; Miltenyi Biotec GmbH, Bergisch Gladbach, Germany., Viganó C; Biozentrum, University of Basel, Basel, Switzerland., von Schubert C; Biozentrum, University of Basel, Basel, Switzerland., Nigg EA; Biozentrum, University of Basel, Basel, Switzerland., Swanton C; Translational Cancer Therapeutics Laboratory, Francis Crick Institute, London, United Kingdom., Sotillo R; Division of Molecular Thoracic Oncology, Deutsches Krebsforschungszentrum (DKFZ), Heidelberg, Germany., Bosio A; Miltenyi Biotec GmbH, Bergisch Gladbach, Germany., Storchová Z; Group Maintenance of Genome Stability, Max Planck Institute of Biochemistry, Martinsried, Germany.; Department of Molecular Genetics, University of Kaiserslautern, Kaiserslautern, Germany., Hardt O; Miltenyi Biotec GmbH, Bergisch Gladbach, Germany. olaf.hardt@miltenyibiotec.de. |
| المصدر: | Cancer research [Cancer Res] 2017 Jun 01; Vol. 77 (11), pp. 2914-2926. Date of Electronic Publication: 2017 Apr 04. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Association for Cancer Research Country of Publication: United States NLM ID: 2984705R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1538-7445 (Electronic) Linking ISSN: 00085472 NLM ISO Abbreviation: Cancer Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Baltimore, Md. : American Association for Cancer Research Original Publication: Chicago [etc.] |
| مواضيع طبية MeSH: | Aneuploidy*, 5'-Nucleotidase/*metabolism , Prion Proteins/*metabolism , Stress, Physiological/*physiology, 5'-Nucleotidase/biosynthesis ; Animals ; Cell Line, Tumor ; Disease Models, Animal ; Humans ; Mice ; Signal Transduction |
| مستخلص: | Aneuploidy is a hallmark of most human tumors, but the molecular physiology of aneuploid cells is not well characterized. In this study, we screened cell surface biomarkers of approximately 300 proteins by multiparameter flow cytometry using multiple aneuploid model systems such as cell lines, patient samples, and mouse models. Several new biomarkers were identified with altered expression in aneuploid cells, including overexpression of the cellular prion protein CD230/PrP C and the immunosuppressive cell surface enzyme ecto-5'-nucleotidase CD73. Functional analyses associated these alterations with increased cellular stress. An increased number of CD73 + cells was observed in confluent cultures in aneuploid cells relative to their diploid counterparts. An elevated expression in CD230/PrP C was observed in serum-deprived cells in association with increased generation of reactive oxygen species. Overall, our work identified biomarkers of aneuploid karyotypes, which suggest insights into the underlying molecular physiology of aneuploid cells. Cancer Res; 77(11); 2914-26. ©2017 AACR . (©2017 American Association for Cancer Research.) |
| المشرفين على المادة: | 0 (Prion Proteins) EC 3.1.3.5 (5'-Nucleotidase) |
| تواريخ الأحداث: | Date Created: 20170406 Date Completed: 20170926 Latest Revision: 20180412 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1158/0008-5472.CAN-16-3052 |
| PMID: | 28377454 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1538-7445 |
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| DOI: | 10.1158/0008-5472.CAN-16-3052 |