دورية أكاديمية
أعرض في EDS

Volatile organic compounds in exhaled breath are independent of systemic inflammatory syndrome caused by intravenous lipopolysaccharide infusion in humans: results from an experiment in healthy volunteers.

التفاصيل البيبلوغرافية
العنوان: Volatile organic compounds in exhaled breath are independent of systemic inflammatory syndrome caused by intravenous lipopolysaccharide infusion in humans: results from an experiment in healthy volunteers.
المؤلفون: Peters AL; Department of Intensive Care Medicine, Academic Medical Center, Meibergdreef 9, 1105 AZ, Amsterdam, The Netherlands., Gerritsen MG, Brinkman P, Zwinderman KAH, Vlaar APJ, Bos LD
المصدر: Journal of breath research [J Breath Res] 2017 Apr 11; Vol. 11 (2), pp. 026003. Date of Electronic Publication: 2017 Apr 11.
نوع المنشور: Clinical Trial; Journal Article
اللغة: English
بيانات الدورية: Publisher: IOP Pub Country of Publication: England NLM ID: 101463871 Publication Model: Electronic Cited Medium: Internet ISSN: 1752-7163 (Electronic) Linking ISSN: 17527155 NLM ISO Abbreviation: J Breath Res Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Bristol, UK : IOP Pub.
مواضيع طبية MeSH: Exhalation* , Healthy Volunteers*, Breath Tests/*methods , Lipopolysaccharides/*administration & dosage , Systemic Inflammatory Response Syndrome/*chemically induced , Systemic Inflammatory Response Syndrome/*diagnosis , Volatile Organic Compounds/*analysis, Adolescent ; Adult ; Biomarkers/metabolism ; Discriminant Analysis ; Gas Chromatography-Mass Spectrometry ; Humans ; Infusions, Intravenous ; Interleukin-6/blood ; Least-Squares Analysis ; Male ; Metabolome ; Respiratory Function Tests ; Systemic Inflammatory Response Syndrome/blood ; Systemic Inflammatory Response Syndrome/physiopathology ; Young Adult
مستخلص: Systemic inflammatory response syndrome (SIRS) is observed during critical illness in most patients. It is defined by a clinical definition. The composition of volatile organic compounds (VOCs) in exhaled breath may change during SIRS and may thus serve as a diagnostic tool. We investigated whether exhaled breath VOCs can serve as biomarker for SIRS in a human model of endotoxemia. Eighteen healthy volunteers received 2 ng Eschericia coli lipopolysaccharide (LPS) kg -1 body weight intravenously. Venous blood and exhaled breath were collected before infusion of LPS and every 2 h thereafter, up to 8 h after infusion. The interleukin (IL)-6 concentration was measured in plasma. VOCs in the exhaled breath were measured by gas chromatography and mass spectrometry. A mixed effects model was fitted to examine the relation between the measured compounds in exhaled breath and time after LPS infusion or IL-6 levels in plasma. Partially-least squares discriminant analysis (PLS-DA) was used to investigate whether we could discriminate between samples collected before and after LPS infusion. The exhaled concentrations of 3-methyl-pentane, 4-methyl-pentanol, 1-hexanol, 2,4-dimethyl-heptane, decane and one unknown compound changed after LPS infusion. However, the false-discovery rate was 43% for the total set of 52 compounds that were present in all samples. Of these VOCs only the unknown compound was associated with systemic levels of IL-6. The PLS-DA algorithm resulted in a moderate discriminatory accuracy. SIRS induced by endotoxemia in human volunteers resulted in minor changes in exhaled VOCs. We therefore conclude that LPS infusion in healthy volunteers does not induce metabolic effects that can be detected through VOC analysis of the exhaled breath. This trial is registered at the Dutch Trial Register: NTR4455.
المشرفين على المادة: 0 (Biomarkers)
0 (IL6 protein, human)
0 (Interleukin-6)
0 (Lipopolysaccharides)
0 (Volatile Organic Compounds)
تواريخ الأحداث: Date Created: 20170412 Date Completed: 20170918 Latest Revision: 20220408
رمز التحديث: 20221213
DOI: 10.1088/1752-7163/aa6545
PMID: 28397711
قاعدة البيانات: MEDLINE
الوصف
تدمد:1752-7163
DOI:10.1088/1752-7163/aa6545