دورية أكاديمية
أعرض في EDS

In vitro detection of cholangiocarcinoma cells using a fluorescent protein-expressing oncolytic herpes virus.

التفاصيل البيبلوغرافية
العنوان: In vitro detection of cholangiocarcinoma cells using a fluorescent protein-expressing oncolytic herpes virus.
المؤلفون: Coelen RJS; Department of Experimental Surgery, Academic Medical Center, Amsterdam, The Netherlands., de Keijzer MJ; Department of Experimental Surgery, Academic Medical Center, Amsterdam, The Netherlands., Weijer R; Department of Experimental Surgery, Academic Medical Center, Amsterdam, The Netherlands., Loukachov VV; Department of Experimental Surgery, Academic Medical Center, Amsterdam, The Netherlands., Wiggers JK; Department of Experimental Surgery, Academic Medical Center, Amsterdam, The Netherlands., Mul FPJ; Department of Research Facilities, Sanquin Research, and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands., van Wijk ACWA; Department of Experimental Surgery, Academic Medical Center, Amsterdam, The Netherlands., Fong Y; Department of Surgery, City of Hope, Duarte, CA, USA., Heger M; Department of Experimental Surgery, Academic Medical Center, Amsterdam, The Netherlands., van Gulik TM; Department of Experimental Surgery, Academic Medical Center, Amsterdam, The Netherlands.
المصدر: Cancer gene therapy [Cancer Gene Ther] 2017 May; Vol. 24 (5), pp. 227-232. Date of Electronic Publication: 2017 Apr 14.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 9432230 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1476-5500 (Electronic) Linking ISSN: 09291903 NLM ISO Abbreviation: Cancer Gene Ther Subsets: MEDLINE
أسماء مطبوعة: Publication: <2002->: London : Nature Publishing Group
Original Publication: Norwalk, CT : Appleton & Lange, c1994-
مواضيع طبية MeSH: Green Fluorescent Proteins/*metabolism , Hepatocytes/*metabolism , Herpesvirus 1, Human/*metabolism , Oncolytic Viruses/*metabolism, Animals ; Bile Acids and Salts/pharmacology ; Bile Duct Neoplasms/genetics ; Bile Duct Neoplasms/metabolism ; Bile Duct Neoplasms/virology ; Cell Line, Tumor ; Cell Survival/drug effects ; Cells, Cultured ; Chlorocebus aethiops ; Cholangiocarcinoma/genetics ; Cholangiocarcinoma/metabolism ; Cholangiocarcinoma/virology ; Flow Cytometry ; Green Fluorescent Proteins/genetics ; Hepatocytes/cytology ; Hepatocytes/virology ; Herpesvirus 1, Human/genetics ; Herpesvirus 1, Human/physiology ; Humans ; Oncolytic Viruses/genetics ; Oncolytic Viruses/physiology ; Vero Cells
مستخلص: Pathological confirmation is desired prior to high-risk surgery for suspected perihilar cholangiocarcinoma (PHC), but preoperative tissue diagnosis is limited by poor sensitivity of available techniques. This study aimed to validate whether a tumor-specific enhanced green fluorescent protein (eGFP)-expressing oncolytic virus could be used for cholangiocarcinoma (CC) cell detection. Extrahepatic CC cell lines SK-ChA-1, EGI-1, TFK-1 and control cells (primary human liver cells) were exposed to the oncolytic herpes simplex type 1 virus NV1066 for up to 24 h in adherent culture. The technique was validated for cells in suspension and cultured cells that had been exposed to crude patient bile. Optimal incubation time of the CC cells with NV1066 at a multiplicity of infection of 0.1 was determined at 6-8 h, yielding 15% eGFP-expressing cells, as measured by flow cytometry. Cells were able to survive 2-h crude bile exposure and remained capable of producing eGFP following NV1066 infection. Detection of malignant cells was possible at the highest dilution tested (10 CC cells among 2 × 10 5 control cells), though hampered by non-target cell autofluorescence. The technique was not applicable to cells in suspension due to insufficient eGFP production. Accordingly, as yet the technique is not suitable for standardized clinical diagnostics in PHC.
References: Hepatology. 2016 Aug;64(2):501-7. (PMID: 27015613)
Ann Surg. 1996 Oct;224(4):463-73; discussion 473-5. (PMID: 8857851)
Endoscopy. 2015 Aug;47(8):739-53. (PMID: 26147492)
J Neurovirol. 2008 Jan;14(1):28-40. (PMID: 18300073)
Cancer Gene Ther. 2006 Mar;13(3):326-34. (PMID: 16138120)
J Histochem Cytochem. 2003 Jan;51(1):5-14. (PMID: 12502749)
Curr Gastroenterol Rep. 2012 Dec;14(6):534-41. (PMID: 23065376)
World J Clin Oncol. 2011 May 10;2(5):203-16. (PMID: 21611097)
Mol Med. 2011;17(7-8):628-34. (PMID: 21487639)
EBioMedicine. 2016 May;7:94-9. (PMID: 27322463)
Surgery. 2009 Sep;146(3):498-505. (PMID: 19715807)
Surgery. 2010 Aug;148(2):325-34. (PMID: 20633729)
Nat Rev Cancer. 2002 Dec;2(12):938-50. (PMID: 12459732)
Surgery. 2014 Aug;156(2):263-9. (PMID: 24957667)
Eur J Surg Oncol. 2011 Jan;37(1):65-71. (PMID: 21115233)
Tohoku J Exp Med. 1995 Sep;177(1):61-71. (PMID: 8693487)
Cancer Gene Ther. 2015 Dec;22(12):591-6. (PMID: 26584530)
Gastrointest Endosc. 2015 Oct;82(4):608-14.e2. (PMID: 26071061)
Nat Protoc. 2008;3(3):363-70. (PMID: 18323807)
Br J Surg. 2011 May;98(5):704-9. (PMID: 21290384)
Br J Surg. 2001 Jan;88(1):48-51. (PMID: 11136309)
Gastrointest Endosc. 2011 Sep;74(3):511-9. (PMID: 21737076)
World J Gastroenterol. 2008 Aug 28;14(32):5032-8. (PMID: 18763286)
Clin Gastroenterol Hepatol. 2012 May;10(5):466-71; quiz e48. (PMID: 22178463)
J Hepatol. 2016 Aug;65(2):305-13. (PMID: 27132171)
Sci Transl Med. 2014 Feb 19;6(224):224ra24. (PMID: 24553385)
World J Surg. 2012 Sep;36(9):2179-86. (PMID: 22569746)
Virol J. 2008 Jun 02;5:68. (PMID: 18518998)
Gastroenterol Rep (Oxf). 2015 Aug;3(3):209-15. (PMID: 25169922)
HPB (Oxford). 2015 Jun;17(6):520-8. (PMID: 25726722)
Hum Gene Ther. 2002 Jul 1;13(10):1213-23. (PMID: 12133274)
Gastrointest Endosc. 2011 Jan;73(1):71-8. (PMID: 21067747)
Nat Protoc. 2006;1(3):1112-6. (PMID: 17406391)
Surgery. 2015 Aug;158(2):331-8. (PMID: 26049609)
J Hepatol. 1985;1(6):579-96. (PMID: 4056357)
المشرفين على المادة: 0 (Bile Acids and Salts)
147336-22-9 (Green Fluorescent Proteins)
تواريخ الأحداث: Date Created: 20170415 Date Completed: 20180227 Latest Revision: 20200306
رمز التحديث: 20231215
DOI: 10.1038/cgt.2017.11
PMID: 28409558
قاعدة البيانات: MEDLINE
الوصف
تدمد:1476-5500
DOI:10.1038/cgt.2017.11