دورية أكاديمية
In vitro detection of cholangiocarcinoma cells using a fluorescent protein-expressing oncolytic herpes virus.
العنوان: | In vitro detection of cholangiocarcinoma cells using a fluorescent protein-expressing oncolytic herpes virus. |
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المؤلفون: | Coelen RJS; Department of Experimental Surgery, Academic Medical Center, Amsterdam, The Netherlands., de Keijzer MJ; Department of Experimental Surgery, Academic Medical Center, Amsterdam, The Netherlands., Weijer R; Department of Experimental Surgery, Academic Medical Center, Amsterdam, The Netherlands., Loukachov VV; Department of Experimental Surgery, Academic Medical Center, Amsterdam, The Netherlands., Wiggers JK; Department of Experimental Surgery, Academic Medical Center, Amsterdam, The Netherlands., Mul FPJ; Department of Research Facilities, Sanquin Research, and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands., van Wijk ACWA; Department of Experimental Surgery, Academic Medical Center, Amsterdam, The Netherlands., Fong Y; Department of Surgery, City of Hope, Duarte, CA, USA., Heger M; Department of Experimental Surgery, Academic Medical Center, Amsterdam, The Netherlands., van Gulik TM; Department of Experimental Surgery, Academic Medical Center, Amsterdam, The Netherlands. |
المصدر: | Cancer gene therapy [Cancer Gene Ther] 2017 May; Vol. 24 (5), pp. 227-232. Date of Electronic Publication: 2017 Apr 14. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 9432230 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1476-5500 (Electronic) Linking ISSN: 09291903 NLM ISO Abbreviation: Cancer Gene Ther Subsets: MEDLINE |
أسماء مطبوعة: | Publication: <2002->: London : Nature Publishing Group Original Publication: Norwalk, CT : Appleton & Lange, c1994- |
مواضيع طبية MeSH: | Green Fluorescent Proteins/*metabolism , Hepatocytes/*metabolism , Herpesvirus 1, Human/*metabolism , Oncolytic Viruses/*metabolism, Animals ; Bile Acids and Salts/pharmacology ; Bile Duct Neoplasms/genetics ; Bile Duct Neoplasms/metabolism ; Bile Duct Neoplasms/virology ; Cell Line, Tumor ; Cell Survival/drug effects ; Cells, Cultured ; Chlorocebus aethiops ; Cholangiocarcinoma/genetics ; Cholangiocarcinoma/metabolism ; Cholangiocarcinoma/virology ; Flow Cytometry ; Green Fluorescent Proteins/genetics ; Hepatocytes/cytology ; Hepatocytes/virology ; Herpesvirus 1, Human/genetics ; Herpesvirus 1, Human/physiology ; Humans ; Oncolytic Viruses/genetics ; Oncolytic Viruses/physiology ; Vero Cells |
مستخلص: | Pathological confirmation is desired prior to high-risk surgery for suspected perihilar cholangiocarcinoma (PHC), but preoperative tissue diagnosis is limited by poor sensitivity of available techniques. This study aimed to validate whether a tumor-specific enhanced green fluorescent protein (eGFP)-expressing oncolytic virus could be used for cholangiocarcinoma (CC) cell detection. Extrahepatic CC cell lines SK-ChA-1, EGI-1, TFK-1 and control cells (primary human liver cells) were exposed to the oncolytic herpes simplex type 1 virus NV1066 for up to 24 h in adherent culture. The technique was validated for cells in suspension and cultured cells that had been exposed to crude patient bile. Optimal incubation time of the CC cells with NV1066 at a multiplicity of infection of 0.1 was determined at 6-8 h, yielding 15% eGFP-expressing cells, as measured by flow cytometry. Cells were able to survive 2-h crude bile exposure and remained capable of producing eGFP following NV1066 infection. Detection of malignant cells was possible at the highest dilution tested (10 CC cells among 2 × 10 5 control cells), though hampered by non-target cell autofluorescence. The technique was not applicable to cells in suspension due to insufficient eGFP production. Accordingly, as yet the technique is not suitable for standardized clinical diagnostics in PHC. |
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المشرفين على المادة: | 0 (Bile Acids and Salts) 147336-22-9 (Green Fluorescent Proteins) |
تواريخ الأحداث: | Date Created: 20170415 Date Completed: 20180227 Latest Revision: 20200306 |
رمز التحديث: | 20231215 |
DOI: | 10.1038/cgt.2017.11 |
PMID: | 28409558 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1476-5500 |
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DOI: | 10.1038/cgt.2017.11 |