دورية أكاديمية
High-resolution myogenic lineage mapping by single-cell mass cytometry.
| العنوان: | High-resolution myogenic lineage mapping by single-cell mass cytometry. |
|---|---|
| المؤلفون: | Porpiglia E; Blau Laboratory, Stanford University School of Medicine, Stanford, California 94305, USA.; Baxter Laboratory for Stem Cell Biology, Stanford University School of Medicine, Stanford, California 94305, USA.; Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, California 94305, USA., Samusik N; Baxter Laboratory for Stem Cell Biology, Stanford University School of Medicine, Stanford, California 94305, USA.; Nolan Laboratory, Stanford University School of Medicine, Stanford, California 94305, USA., Ho ATV; Blau Laboratory, Stanford University School of Medicine, Stanford, California 94305, USA.; Baxter Laboratory for Stem Cell Biology, Stanford University School of Medicine, Stanford, California 94305, USA.; Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, California 94305, USA., Cosgrove BD; Blau Laboratory, Stanford University School of Medicine, Stanford, California 94305, USA.; Baxter Laboratory for Stem Cell Biology, Stanford University School of Medicine, Stanford, California 94305, USA.; Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, California 94305, USA., Mai T; Blau Laboratory, Stanford University School of Medicine, Stanford, California 94305, USA.; Baxter Laboratory for Stem Cell Biology, Stanford University School of Medicine, Stanford, California 94305, USA.; Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, California 94305, USA., Davis KL; Baxter Laboratory for Stem Cell Biology, Stanford University School of Medicine, Stanford, California 94305, USA.; Nolan Laboratory, Stanford University School of Medicine, Stanford, California 94305, USA., Jager A; Baxter Laboratory for Stem Cell Biology, Stanford University School of Medicine, Stanford, California 94305, USA.; Nolan Laboratory, Stanford University School of Medicine, Stanford, California 94305, USA., Nolan GP; Baxter Laboratory for Stem Cell Biology, Stanford University School of Medicine, Stanford, California 94305, USA.; Nolan Laboratory, Stanford University School of Medicine, Stanford, California 94305, USA., Bendall SC; Baxter Laboratory for Stem Cell Biology, Stanford University School of Medicine, Stanford, California 94305, USA.; Nolan Laboratory, Stanford University School of Medicine, Stanford, California 94305, USA., Fantl WJ; Baxter Laboratory for Stem Cell Biology, Stanford University School of Medicine, Stanford, California 94305, USA.; Stanford Comprehensive Cancer Institute and Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford California, California 94305, USA., Blau HM; Blau Laboratory, Stanford University School of Medicine, Stanford, California 94305, USA.; Baxter Laboratory for Stem Cell Biology, Stanford University School of Medicine, Stanford, California 94305, USA.; Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, California 94305, USA. |
| المصدر: | Nature cell biology [Nat Cell Biol] 2017 May; Vol. 19 (5), pp. 558-567. Date of Electronic Publication: 2017 Apr 17. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Macmillan Magazines Ltd Country of Publication: England NLM ID: 100890575 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1476-4679 (Electronic) Linking ISSN: 14657392 NLM ISO Abbreviation: Nat Cell Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London : Macmillan Magazines Ltd., [1999- |
| مواضيع طبية MeSH: | Cell Lineage* , Muscle Development*/drug effects , Regeneration*/drug effects, Cell Separation/*methods , Flow Cytometry/*methods , Muscle, Skeletal/*metabolism , Myoblasts, Skeletal/*metabolism , Single-Cell Analysis/*methods , Stem Cells/*metabolism, Animals ; Biomarkers/metabolism ; Cell Proliferation ; Cells, Cultured ; Elapid Venoms/toxicity ; Fusion Regulatory Protein-1/metabolism ; Genes, Reporter ; Genotype ; High-Throughput Screening Assays ; Hyaluronan Receptors/metabolism ; Integrin beta4/metabolism ; Luminescent Proteins/genetics ; Luminescent Proteins/metabolism ; Mice, Inbred C57BL ; Mice, Transgenic ; Muscle, Skeletal/drug effects ; Muscle, Skeletal/injuries ; Muscle, Skeletal/pathology ; MyoD Protein/metabolism ; Myoblasts, Skeletal/drug effects ; Myoblasts, Skeletal/pathology ; PAX7 Transcription Factor/deficiency ; PAX7 Transcription Factor/genetics ; Phenotype ; Stem Cells/drug effects ; Stem Cells/pathology ; Tetraspanin 29/metabolism ; Time Factors |
| مستخلص: | Muscle regeneration is a dynamic process during which cell state and identity change over time. A major roadblock has been a lack of tools to resolve a myogenic progression in vivo. Here we capitalize on a transformative technology, single-cell mass cytometry (CyTOF), to identify in vivo skeletal muscle stem cell and previously unrecognized progenitor populations that precede differentiation. We discovered two cell surface markers, CD9 and CD104, whose combined expression enabled in vivo identification and prospective isolation of stem and progenitor cells. Data analysis using the X-shift algorithm paired with single-cell force-directed layout visualization defined a molecular signature of the activated stem cell state (CD44 + /CD98 + /MyoD + ) and delineated a myogenic trajectory during recovery from acute muscle injury. Our studies uncover the dynamics of skeletal muscle regeneration in vivo and pave the way for the elucidation of the regulatory networks that underlie cell-state transitions in muscle diseases and ageing. |
| التعليقات: | Erratum in: Nat Cell Biol. 2018 Mar 5;:. (PMID: 29507406) |
| References: | Blood. 2011 Feb 10;117(6):1840-50. (PMID: 21063023) Front Cell Dev Biol. 2016 Aug 30;4:91. (PMID: 27626031) Lab Invest. 2015 Mar;95(3):308-19. (PMID: 25599535) Stem Cells. 2008 Dec;26(12):3194-204. (PMID: 18802040) Cell. 2011 Jan 7;144(1):92-105. (PMID: 21215372) Nat Med. 2015 Aug;21(8):854-62. (PMID: 26248268) Cell. 2006 Aug 11;126(3):489-502. (PMID: 16901783) Cell. 2015 Jul 2;162(1):184-97. (PMID: 26095251) Development. 2013 Feb;140(4):689-704. (PMID: 23362343) Proc Natl Acad Sci U S A. 2013 Oct 8;110(41):16474-9. (PMID: 24065826) Cell. 2012 Jan 20;148(1-2):112-25. (PMID: 22265406) Development. 2012 Aug;139(16):2845-56. (PMID: 22833472) Nat Protoc. 2015 Oct;10(10):1612-24. (PMID: 26401916) J Cell Physiol. 2006 Nov;209(2):314-21. (PMID: 16906571) Cell Mol Life Sci. 2011 Oct;68(19):3275-92. (PMID: 21365281) Neuromolecular Med. 2008;10(4):316-21. (PMID: 18516507) J Biol Chem. 2015 Nov 20;290(47):28456-64. (PMID: 26453297) Cell. 2014 May 8;157(4):935-49. (PMID: 24813615) Cell. 2014 Apr 24;157(3):714-25. (PMID: 24766814) Cell Stem Cell. 2016 Dec 1;19(6):800-807. (PMID: 27641304) Blood. 2008 Dec 15;112(13):4808-17. (PMID: 19064740) Cell Adhes Commun. 1994 Apr;2(1):1-6. (PMID: 7982032) J Cell Biol. 1999 Aug 23;146(4):893-904. (PMID: 10459022) Nat Genet. 1996 Jul;13(3):366-9. (PMID: 8673140) Cell. 2016 May 5;165(4):780-91. (PMID: 27153492) Nat Methods. 2016 Jun;13(6):493-6. (PMID: 27183440) Curr Top Dev Biol. 2011;94:197-234. (PMID: 21295688) Elife. 2015 Mar 27;4:e03390. (PMID: 25815583) Cell. 2000 Sep 15;102(6):777-86. (PMID: 11030621) Cell Rep. 2013 Aug 29;4(4):642-8. (PMID: 23954783) PLoS Biol. 2007 May;5(5):e102. (PMID: 17439301) J Cell Biol. 2005 Apr 11;169(1):105-16. (PMID: 15824134) Science. 2010 Aug 27;329(5995):1078-81. (PMID: 20647425) Cell Stem Cell. 2012 Oct 5;11(4):541-53. (PMID: 23040480) Cell. 2008 Jul 11;134(1):37-47. (PMID: 18614009) J Immunol. 2008 Nov 15;181(10):7002-13. (PMID: 18981120) Cell Stem Cell. 2015 Mar 5;16(3):323-37. (PMID: 25748935) J Histochem Cytochem. 2012 Jan;60(1):31-44. (PMID: 22205679) Cytometry A. 2012 Jul;81(7):552-66. (PMID: 22693166) Curr Top Dev Biol. 2014;107:161-81. (PMID: 24439806) Stem Cell Reports. 2016 Aug 9;7(2):263-78. (PMID: 27509136) Nature. 2008 Nov 27;456(7221):502-6. (PMID: 18806774) Science. 2011 May 6;332(6030):687-96. (PMID: 21551058) Nat Med. 2014 Mar;20(3):255-64. (PMID: 24531378) Science. 2000 Feb 25;287(5457):1442-6. (PMID: 10688785) Am J Physiol Endocrinol Metab. 2010 May;298(5):E1011-8. (PMID: 20304764) PLoS One. 2014 Jun 10;9(6):e98679. (PMID: 24914678) |
| معلومات مُعتمدة: | K99 AG042491 United States AG NIA NIH HHS; R00 AG042491 United States AG NIA NIH HHS; R01 AG020961 United States AG NIA NIH HHS; R01 NS089533 United States NS NINDS NIH HHS |
| المشرفين على المادة: | 0 (Biomarkers) 0 (Cd44 protein, mouse) 0 (Cd9 protein, mouse) 0 (Elapid Venoms) 0 (Fusion Regulatory Protein-1) 0 (Hyaluronan Receptors) 0 (Integrin beta4) 0 (Luminescent Proteins) 0 (MyoD Protein) 0 (MyoD1 myogenic differentiation protein) 0 (PAX7 Transcription Factor) 0 (Pax7 protein, mouse) 0 (Tetraspanin 29) 37223-96-4 (notexin) |
| تواريخ الأحداث: | Date Created: 20170418 Date Completed: 20170908 Latest Revision: 20200306 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC5728993 |
| DOI: | 10.1038/ncb3507 |
| PMID: | 28414312 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1476-4679 |
|---|---|
| DOI: | 10.1038/ncb3507 |