دورية أكاديمية
Expression and Purification of Delta Sleep-Inducing Peptide Fused with Protein Transduction Domain and Human Serum Albumin in Pichia pastoris.
| العنوان: | Expression and Purification of Delta Sleep-Inducing Peptide Fused with Protein Transduction Domain and Human Serum Albumin in Pichia pastoris. |
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| المؤلفون: | Zhang XG; School of Life Science and Engineering, Lanzhou University of Technology, No.287 Langongping Road, Qilihe District, Lanzhou City, Gansu Province. China., Wang WN; School of Life Science and Engineering, Lanzhou University of Technology; Key Laboratory of Screening and Processing in new Tibetan medicine of Gansu Province, Gansu, 730050. China., Zhang CS; School of Life Science and Engineering, Lanzhou University of Technology; Key Laboratory of Screening and Processing in new Tibetan medicine of Gansu Province, Gansu, 730050. China., Li K; School of Life Science and Engineering, Lanzhou University of Technology; Key Laboratory of Screening and Processing in new Tibetan medicine of Gansu Province, Gansu, 730050. China., Ma GD; School of Life Science and Engineering, Lanzhou University of Technology; Key Laboratory of Screening and Processing in new Tibetan medicine of Gansu Province, Gansu, 730050. China., Li JY; Key Lab of New Animal Drug Project, Gansu Province; Key Lab of Veterinary Pharmaceutical Development, Ministry of Agriculture; Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS, Lanzhou, Gansu 730050. China. |
| المصدر: | Protein and peptide letters [Protein Pept Lett] 2017; Vol. 24 (7), pp. 668-675. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Bentham Science Publishers Country of Publication: Netherlands NLM ID: 9441434 Publication Model: Print Cited Medium: Internet ISSN: 1875-5305 (Electronic) Linking ISSN: 09298665 NLM ISO Abbreviation: Protein Pept Lett Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Hilversum, The Netherlands : Bentham Science Publishers Original Publication: Schiphol, The Netherlands : Bentham Science Publishers, c1994- |
| مواضيع طبية MeSH: | Delta Sleep-Inducing Peptide/*administration & dosage , Recombinant Fusion Proteins/*administration & dosage , Sleep/*drug effects , Sleep Initiation and Maintenance Disorders/*drug therapy, Animals ; Delta Sleep-Inducing Peptide/chemistry ; Delta Sleep-Inducing Peptide/genetics ; Humans ; Mice ; Pentobarbital/administration & dosage ; Pichia/genetics ; Protein Domains ; Recombinant Fusion Proteins/chemistry ; Recombinant Fusion Proteins/genetics ; Serum Albumin/administration & dosage ; Serum Albumin/chemistry ; Serum Albumin/genetics ; Sleep Initiation and Maintenance Disorders/pathology |
| مستخلص: | Background: Sleep is a natural part of every individual's life. Delta sleep-inducing peptide (DSIP) is a nonapeptide that could promote sleep through the induction of slow wave sleep. However, little is known about the pharmacological effect of DSIP on insomnia. Objectives: The main objective of this study was to analyze the pharmacological effect of DSIP on insomnia. Methods: We designed a fusion protein containing N-terminal TAT-based transduction domain followed by human serum albumin and DSIP and designated this protein as PHD fusion protein. The PHD fusion protein were expressed in Pichia pastoris and purified. Mice were administered single subcutaneous injections three concentrations of PHD fusion protein (0.5, 1, 2 mg/kg), and the pharmacological activity of PHD fusion protein was studied using classic pentobarbitalinduced sleep test. Results: We expressed the PHD fusion protein in P. pastoris; furthermore, the PHD fused protein was purified to near homogeneity by DEAE Sepharose FF, Phenyl Sepharose HP and Blue Sepharose 6 FF. Our result showed that the increase of pentobarbital-induced hypnotic effect characterized by reducing sleep latency and prolonged sleep duration was observed for increasing concentrations of PHD fusion protein (P<0.05); moreover, different dose of PHD fusion protein could induce the mice to re-sleep in a dose-dependent manner, whereas higher doses of PHD fusion protein (1.0, 2.0 mg/kg) significantly increased the rate of sleep re-onset compared with the vehicle group of mice (P<0.05). Conclusion: PHD fusion protein increased the hypnotic effects of pentobarbital by reducing sleep latency and prolonged sleep duration. The present study suggested PHD fusion protein could be a new drug candidate for insomnia. (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.) |
| فهرسة مساهمة: | Keywords: Delta sleep-inducing peptide; Pichia pastoris; fusion protein; human serum albumin; protein transduction domain |
| المشرفين على المادة: | 0 (Recombinant Fusion Proteins) 0 (Serum Albumin) 69431-45-4 (Delta Sleep-Inducing Peptide) I4744080IR (Pentobarbital) |
| تواريخ الأحداث: | Date Created: 20170503 Date Completed: 20171024 Latest Revision: 20171024 |
| رمز التحديث: | 20240628 |
| DOI: | 10.2174/0929866524666170426113022 |
| PMID: | 28462721 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1875-5305 |
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| DOI: | 10.2174/0929866524666170426113022 |