دورية أكاديمية
OPTIMIZATION OF ALKALINE Α-AMYLASE PRODUCTION BY THERMOPHILIC BACILL US SUBTILIS .
| العنوان: | OPTIMIZATION OF ALKALINE Α-AMYLASE PRODUCTION BY THERMOPHILIC BACILL US SUBTILIS . |
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| المؤلفون: | Al-Johani NB; Biochemistry Department, Faculty of Science. King Abdulaziz University, Jeddah, Saudi Arabia., Al-Seeni MN; Biochemistry Department, Faculty of Science. King Abdulaziz University, Jeddah, Saudi Arabia., Ahmed YM; Biochemistry Department, Faculty of Science. King Abdulaziz University, Jeddah, Saudi Arabia.; Production of Bioproducts for Industrial Applications Research Group and Experimental Biochemistry Unit, King Fahd Medical Research Center, King Abdulaziz University, KSA.; Microbial Biotechnology Dep., Genetic Engineering and Biotechnology Research division, National Research Center, Dokki -Cairo. |
| المصدر: | African journal of traditional, complementary, and alternative medicines : AJTCAM [Afr J Tradit Complement Altern Med] 2016 Nov 23; Vol. 14 (1), pp. 288-301. Date of Electronic Publication: 2016 Nov 23 (Print Publication: 2017). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: African Traditional Herbal Medicine Supporters Initiative Country of Publication: Nigeria NLM ID: 101232990 Publication Model: eCollection Cited Medium: Internet ISSN: 2505-0044 (Electronic) Linking ISSN: 25050044 NLM ISO Abbreviation: Afr J Tradit Complement Altern Med Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : Osun : African Traditional Herbal Medicine Supporters Initiative Original Publication: [Ile-Ife, Nigeria? : African Networks on Ethnomedicines, 2004]- |
| مواضيع طبية MeSH: | Bacillus subtilis/*enzymology , Bacterial Proteins/*chemistry , Bacterial Proteins/*metabolism , Hot Springs/*microbiology , alpha-Amylases/*chemistry , alpha-Amylases/*metabolism, Bacillus subtilis/classification ; Bacillus subtilis/genetics ; Bacillus subtilis/isolation & purification ; Bacterial Proteins/genetics ; Enzyme Stability ; Hydrogen-Ion Concentration ; Temperature ; alpha-Amylases/genetics |
| مستخلص: | Background: Starch-degrading amylase enzyme is important in biotechnological applications as food, fermentation, textile, paper and pharmaceutical purposes. The aim of current study to isolate alkaline thermostable α-amylase bacteria and then study the composition of medium and culture conditions to optimize cells growth and a-amylase production. Materials and Methods: Thermophilic amylase producing bacterium was isolated from local hot water-springs in Gazan city Saudi Arabia. Results: Phylogenetic analysis of 16 S rRNA sequence for the strain revealed that the strain have the same sequence of Bacillus subtilis . Maximum amylase production was observed, when B. subtilis cultured in medium containing starch at concentration 0.5%, and 10 g/L peptones as nitrogen source at pH 8.5 in when it was incubated for 48 h at 45°C. Conclusion: An amylase-producing bacterium were isolated from hot-spring water and was identified as B. subtilis . Amylase produced from B.subtilis had optimum temperature 45°C and pH 8.5 in shaking media. |
| References: | Appl Microbiol Biotechnol. 2010 Feb;85(5):1437-50. (PMID: 19669756) Bioresour Technol. 2007 Jan;98(1):150-7. (PMID: 16376073) Pak J Biol Sci. 2007 Jun 15;10(12):2039-47. (PMID: 19093444) Appl Environ Microbiol. 1986 Jul;52(1):179-84. (PMID: 16347107) Int J Biol Macromol. 2012 Jan 1;50(1):219-29. (PMID: 22085756) J Appl Microbiol. 1997 Mar;82(3):325-34. (PMID: 12455896) Hepatol Res. 2007 Feb;37(2):113-20. (PMID: 17300706) Biochem Res Int. 2014;2014:568141. (PMID: 24672727) Iran J Microbiol. 2010 Mar;2(1):46-50. (PMID: 22347550) Biosci Biotechnol Biochem. 2006 Feb;70(2):433-40. (PMID: 16495660) Biotechnol Appl Biochem. 1998 Aug;28 ( Pt 1):61-8. (PMID: 9693090) Biotechnol Appl Biochem. 2000 Apr;31 ( Pt 2):135-52. (PMID: 10744959) Bioresour Technol. 2007 Jan;98(2):260-5. (PMID: 16524725) Appl Biochem Biotechnol. 2010 Apr;160(8):2401-14. (PMID: 19763902) Bioresour Technol. 2004 Jun;93(2):169-74. (PMID: 15051078) |
| فهرسة مساهمة: | Keywords: Bacillus subtilis; Thermophilic bacteria; α-amylase |
| المشرفين على المادة: | 0 (Bacterial Proteins) EC 3.2.1.1 (alpha-Amylases) |
| تواريخ الأحداث: | Date Created: 20170509 Date Completed: 20170626 Latest Revision: 20181113 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC5411881 |
| DOI: | 10.21010/ajtcam.v14i1.31 |
| PMID: | 28480407 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2505-0044 |
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| DOI: | 10.21010/ajtcam.v14i1.31 |