دورية أكاديمية
Sodium louroyl sarcosinate (sarkosyl) modulate amyloid fibril formation in hen egg white lysozyme (HEWL) at alkaline pH: a molecular insight study.
| العنوان: | Sodium louroyl sarcosinate (sarkosyl) modulate amyloid fibril formation in hen egg white lysozyme (HEWL) at alkaline pH: a molecular insight study. |
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| المؤلفون: | Khan JM; a Faculty of Food and Agricultural Sciences, Department of Food Science and Nutrition , King Saud University , 2460 Riyadh 11451 , Saudi Arabia., Khan MS; b Department of Biochemistry, College of Science , King Saud University , Riyadh , Saudi Arabia., Alsenaidy MA; c Department of Pharmaceutics, College of Pharmacy , King Saud University , Riyadh , Saudi Arabia., Ahmed A; b Department of Biochemistry, College of Science , King Saud University , Riyadh , Saudi Arabia., Sen P; d Centre for Bioseparation Technology , VIT University , Vellore 632014 , India., Oves M; e Center of Excellence in Enviromental Studies , King Abdulaziz University , Jeddah , Saudi Arabia., Al-Shabib NA; a Faculty of Food and Agricultural Sciences, Department of Food Science and Nutrition , King Saud University , 2460 Riyadh 11451 , Saudi Arabia., Khan RH; f Interdisciplinary Biotechnology Unit , Aligarh Muslim University , Aligarh , India. |
| المصدر: | Journal of biomolecular structure & dynamics [J Biomol Struct Dyn] 2018 May; Vol. 36 (6), pp. 1550-1565. Date of Electronic Publication: 2017 May 28. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Taylor & Francis Country of Publication: England NLM ID: 8404176 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1538-0254 (Electronic) Linking ISSN: 07391102 NLM ISO Abbreviation: J Biomol Struct Dyn Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: June 2012- : Oxon, UK : Taylor & Francis Original Publication: Guilderland, NY : Adenine Press, [c1983- |
| مواضيع طبية MeSH: | Amyloid/*chemistry , Muramidase/*chemistry , Sarcosine/*analogs & derivatives , Sarcosine/*chemistry, Animals ; Circular Dichroism/methods ; Dynamic Light Scattering/methods ; Fluorescence ; Hydrogen-Ion Concentration ; Hydrophobic and Hydrophilic Interactions ; Microscopy, Atomic Force/methods ; Molecular Docking Simulation/methods ; Protein Aggregates ; Static Electricity ; Surface-Active Agents/chemistry |
| مستخلص: | Amyloid fibril formation is responsible for several neurodegenerative diseases and are formed when native proteins misfold and stick together with different interactive forces. In the present study, we have determined the mode of interaction of the anionic surfactant sarkosyl with hen egg white lysozyme (HEWL) [EC No. 3.2.1.17] at two pHs (9.0 and 13.0) and investigated its impact on fibrillogenesis. Our data suggested that sarkosyl is promoting amyloid fibril formation in HEWL at the concentration range between 0.9 and 3.0 mM and no amyloid fibril formation was observed in the concentration range of 3.0-20.0 mM at pH 9.0. The results were confirmed by several biophysical and computational techniques, such as turbidity measurement, dynamic light scattering, Raleigh scattering, ThT fluorescence, intrinsic fluorescence, far-UV CD and atomic force microscopy. Sarkosyl was unable to induce aggregation in HEWL at pH 13.0 as confirmed by turbidity and RLS measurements. HEWL forms larger amyloid fibrils in the presence of 1.6 mM of sarkosyl. The spectroscopic, microscopic and molecular docking data suggest that the negatively charged carboxylate group and 12-carbon hydrophobic tail of sarkosyl stimulate amyloid fibril formation in HEWL via electrostatic and hydrophobic interaction. This study leads to new insight into the process of suppression of fibrillogenesis in HEWL which can be prevented by designing ligands that can retard the electrostatic and hydrophobic interaction between sarkosyl and HEWL. |
| فهرسة مساهمة: | Keywords: amyloid fibril formation and ThT; hen egg white lysozyme; protein aggregation; sarkosyl; surfactant |
| المشرفين على المادة: | 0 (Amyloid) 0 (Protein Aggregates) 0 (Surface-Active Agents) 632GS99618 (sarkosyl) EC 3.2.1.- (hen egg lysozyme) EC 3.2.1.17 (Muramidase) Z711V88R5F (Sarcosine) |
| تواريخ الأحداث: | Date Created: 20170512 Date Completed: 20190131 Latest Revision: 20190131 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1080/07391102.2017.1329097 |
| PMID: | 28490248 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1538-0254 |
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| DOI: | 10.1080/07391102.2017.1329097 |