دورية أكاديمية
Oligonucleotide probes functionalization of nanogap electrodes.
| العنوان: | Oligonucleotide probes functionalization of nanogap electrodes. |
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| المؤلفون: | Zaffino RL; Nanobioengineering Group, Institute for Bioengineering of Catalonia (IBEC), Barcelona, Spain.; Department of Engineering: Electronics, University of Barcelona, Barcelona, Spain., Mir M; Nanobioengineering Group, Institute for Bioengineering of Catalonia (IBEC), Barcelona, Spain.; Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Spain., Samitier J; Nanobioengineering Group, Institute for Bioengineering of Catalonia (IBEC), Barcelona, Spain.; Department of Engineering: Electronics, University of Barcelona, Barcelona, Spain.; Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Spain. |
| المصدر: | Electrophoresis [Electrophoresis] 2017 Nov; Vol. 38 (21), pp. 2712-2720. Date of Electronic Publication: 2017 Jun 05. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 8204476 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1522-2683 (Electronic) Linking ISSN: 01730835 NLM ISO Abbreviation: Electrophoresis Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Original Publication: [Weinheim, Germany] : Verlag Chemie, [1980- |
| مواضيع طبية MeSH: | Biosensing Techniques/*instrumentation , DNA/*analysis , Nanostructures/*chemistry , Oligonucleotide Probes/*chemistry, Electrochemical Techniques/instrumentation ; Electrodes ; Electromagnetic Fields ; Electrophoresis/instrumentation ; Gold/chemistry ; Humans ; Nanotechnology ; Silicon Dioxide/chemistry ; Surface Properties ; Titanium/chemistry |
| مستخلص: | Nanogap electrodes have attracted a lot of consideration as promising platform for molecular electronic and biomolecules detection. This is mainly for their higher aspect ratio, and because their electrical properties are easily accessed by current-voltage measurements. Nevertheless, application of standard current-voltages measurements used to characterize nanogap response, and/or to modify specific nanogap electrodes properties, represents an issue. Since the strength of electrical fields in nanoscaled devices can reach high values, even at low voltages. Here, we analyzed the effects induced by different methods of surface modification of nanogap electrodes, in test-voltage application, employed for the electrical detection of a desoxyribonucleic acid (DNA) target. Nanogap electrodes were functionalized with two antisymmetric oligo-probes designed to have 20 terminal bases complementary to the edges of the target, which after hybridization bridges the nanogap, closing the electrical circuit. Two methods of functionalization were studied for this purpose; a random self-assembling of a mixture of the two oligo-probes (OPs) used in the platform, and a selective method that controls the position of each OP at selected side of nanogap electrodes. We used for this aim, the electrophoretic effect induced on negatively charged probes by the application of an external direct current voltage. The results obtained with both functionalization methods where characterized and compared in terms of electrode surface covering, calculated by using voltammetry analysis. Moreover, we contrasted the electrical detection of a DNA target in the nanogap platform either in site-selective and in randomly assembled nanogap. According to our results, a denser, although not selective surface functionalization, is advantageous for such kind of applications. (© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.) |
| فهرسة مساهمة: | Keywords: Biosensor bioelectronics; DNA electrophoresis; Nanogap electrodes; Self-assembled monolayers; Site-selective deposition |
| المشرفين على المادة: | 0 (Oligonucleotide Probes) 7440-57-5 (Gold) 7631-86-9 (Silicon Dioxide) 9007-49-2 (DNA) D1JT611TNE (Titanium) |
| تواريخ الأحداث: | Date Created: 20170516 Date Completed: 20180427 Latest Revision: 20180427 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1002/elps.201600554 |
| PMID: | 28504351 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1522-2683 |
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| DOI: | 10.1002/elps.201600554 |