دورية أكاديمية
Species level identification of coagulase negative Staphylococcus spp. from buffalo using matrix-assisted laser desorption ionization-time of flight mass spectrometry and cydB real-time quantitative PCR.
| العنوان: | Species level identification of coagulase negative Staphylococcus spp. from buffalo using matrix-assisted laser desorption ionization-time of flight mass spectrometry and cydB real-time quantitative PCR. |
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| المؤلفون: | Pizauro LJL; Department of Veterinary Preventive Medicine and Animal Reproduction, São Paulo State University (Unesp), School of Agricultural and Veterinarian Sciences, Jaboticabal, Brazil., de Almeida CC; Department of Veterinary Pathology, São Paulo State University (Unesp), School of Agricultural and Veterinarian Sciences, Jaboticabal, Brazil., Soltes GA; University of Guelph, Department of Pathobiology, 50 Stone Rd. East, Guelph, Ontario, N1G 2W1, Canada., Slavic D; Animal Health Laboratory, University of Guelph, Post Office 3612, Guelph, Ontario, N1H 6R8, Canada., Rossi-Junior OD; Department of Veterinary Preventive Medicine and Animal Reproduction, São Paulo State University (Unesp), School of Agricultural and Veterinarian Sciences, Jaboticabal, Brazil., de Ávila FA; Department of Veterinary Pathology, São Paulo State University (Unesp), School of Agricultural and Veterinarian Sciences, Jaboticabal, Brazil., Zafalon LF; Brazilian Agricultural Research Corporation (EMBRAPA), Embrapa Southeast Livestock, Washington Luiz road, Km 234, 13560-970, Sao Carlos, Sao Paulo, Brazil., MacInnes JI; University of Guelph, Department of Pathobiology, 50 Stone Rd. East, Guelph, Ontario, N1G 2W1, Canada. Electronic address: macinnes@uoguelph.ca. |
| المصدر: | Veterinary microbiology [Vet Microbiol] 2017 May; Vol. 204, pp. 8-14. Date of Electronic Publication: 2017 Apr 09. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Scientific Pub. Co Country of Publication: Netherlands NLM ID: 7705469 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2542 (Electronic) Linking ISSN: 03781135 NLM ISO Abbreviation: Vet Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier Scientific Pub. Co. |
| مواضيع طبية MeSH: | Buffaloes/*microbiology , Cytochromes/*genetics , Polymerase Chain Reaction/*methods , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*veterinary , Staphylococcus/*genetics, Animals ; Base Sequence ; DNA, Bacterial/genetics ; DNA, Bacterial/isolation & purification ; Electron Transport Chain Complex Proteins/genetics ; Female ; Gene Expression Regulation, Bacterial/physiology ; Gene Expression Regulation, Enzymologic/physiology ; Genetic Variation ; Oxidoreductases/genetics ; Staphylococcus/classification |
| مستخلص: | Incorrect identification of Staphylococcus spp. can have serious clinical and zoonotic repercussions. Accordingly, the aim of this study was to determine if matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and/or cydB real- time quantitative PCR (qPCR) could be used to accurately identify coagulase negative Staphylococcus spp. (CoNS) obtained from buffalo milk and milking environment samples. Seventy-five of 84 CoNS isolates could be identified to the species level (score value >1.99) using MALDI-TOF MS. However, as determined by cytochrome d ubiquinol oxidase subunit II (cydB) qPCR and by 16S RNA and cydB gene sequencing, 10S. agnetis strains were wrongly identified as S. hyicus by MALDI-TOF MS. In addition, 9 isolates identified by MALDI-TOF only to the genus level (score values between 1.70 and 1.99) could be identified to species by cydB qPCR. Our findings suggest that MALDI-TOF MS is a reliable method for rapid identification of S. chromogenes and S. epidermidis (species of interest both in human and veterinary medicine) and may be able to correctly identify other Staphylococcus spp. However, at present not all Staphylococcus spp. found in buffalo milk can be accurately identified by MALDI-TOF MS and for these organisms, the cydB qPCR developed in the current study may provide a reliable alternative method for rapid identification of CoNS species. (Copyright © 2017 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: MALDI-TOF MS; Milk; Staphylococcus agnetis; Staphylococcus hyicus; cydB qPCR |
| المشرفين على المادة: | 0 (Cytochromes) 0 (DNA, Bacterial) 0 (Electron Transport Chain Complex Proteins) EC 1.- (Oxidoreductases) |
| تواريخ الأحداث: | Date Created: 20170524 Date Completed: 20180103 Latest Revision: 20180103 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.vetmic.2017.03.036 |
| PMID: | 28532810 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-2542 |
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| DOI: | 10.1016/j.vetmic.2017.03.036 |