دورية أكاديمية
أعرض في EDS

Environmental DNA (eDNA) metabarcoding assays to detect invasive invertebrate species in the Great Lakes.

التفاصيل البيبلوغرافية
العنوان: Environmental DNA (eDNA) metabarcoding assays to detect invasive invertebrate species in the Great Lakes.
المؤلفون: Klymus KE; Great Lakes Genetics/Genomics Laboratory, Department of Environmental Sciences, University of Toledo, Toledo, OH, United States of America., Marshall NT; Great Lakes Genetics/Genomics Laboratory, Department of Environmental Sciences, University of Toledo, Toledo, OH, United States of America., Stepien CA; Great Lakes Genetics/Genomics Laboratory, Department of Environmental Sciences, University of Toledo, Toledo, OH, United States of America.
المصدر: PloS one [PLoS One] 2017 May 18; Vol. 12 (5), pp. e0177643. Date of Electronic Publication: 2017 May 18 (Print Publication: 2017).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Biodiversity* , DNA Barcoding, Taxonomic* , Environment* , Introduced Species* , Lakes*, Invertebrates/*classification , Invertebrates/*genetics, Animals ; DNA Primers/genetics ; Environmental Monitoring ; Sequence Analysis, DNA
مستخلص: Describing and monitoring biodiversity comprise integral parts of ecosystem management. Recent research coupling metabarcoding and environmental DNA (eDNA) demonstrate that these methods can serve as important tools for surveying biodiversity, while significantly decreasing the time, expense and resources spent on traditional survey methods. The literature emphasizes the importance of genetic marker development, as the markers dictate the applicability, sensitivity and resolution ability of an eDNA assay. The present study developed two metabarcoding eDNA assays using the mtDNA 16S RNA gene with Illumina MiSeq platform to detect invertebrate fauna in the Laurentian Great Lakes and surrounding waterways, with a focus for use on invasive bivalve and gastropod species monitoring. We employed careful primer design and in vitro testing with mock communities to assess ability of the markers to amplify and sequence targeted species DNA, while retaining rank abundance information. In our mock communities, read abundances reflected the initial input abundance, with regressions having significant slopes (p<0.05) and high coefficients of determination (R2) for all comparisons. Tests on field environmental samples revealed similar ability of our markers to measure relative abundance. Due to the limited reference sequence data available for these invertebrate species, care must be taken when analyzing results and identifying sequence reads to species level. These markers extend eDNA metabarcoding research for molluscs and appear relevant to other invertebrate taxa, such as rotifers and bryozoans. Furthermore, the sphaeriid mussel assay is group-specific, exclusively amplifying bivalves in the Sphaeridae family and providing species-level identification. Our assays provide useful tools for managers and conservation scientists, facilitating early detection of invasive species as well as improving resolution of mollusc diversity.
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المشرفين على المادة: 0 (DNA Primers)
تواريخ الأحداث: Date Created: 20170526 Date Completed: 20170912 Latest Revision: 20240326
رمز التحديث: 20240326
مُعرف محوري في PubMed: PMC5436814
DOI: 10.1371/journal.pone.0177643
PMID: 28542313
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0177643