دورية أكاديمية
Estrogen receptor β regulates the tumoral suppressor PTEN to modulate pituitary cell growth.
| العنوان: | Estrogen receptor β regulates the tumoral suppressor PTEN to modulate pituitary cell growth. |
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| المؤلفون: | Perez PA; Centro de Microscopia Electrónica, Instituto de Investigaciones en Ciencias de la Salud (INICSA-CONICET), Facultad de Ciencias Medicas, Universidad Nacional de Cordoba, Cordoba, Argentina., Petiti JP, Picech F; Centro de Microscopia Electrónica, Instituto de Investigaciones en Ciencias de la Salud (INICSA-CONICET), Facultad de Ciencias Medicas, Universidad Nacional de Cordoba, Cordoba, Argentina., Guido CB; Centro de Microscopia Electrónica, Instituto de Investigaciones en Ciencias de la Salud (INICSA-CONICET), Facultad de Ciencias Medicas, Universidad Nacional de Cordoba, Cordoba, Argentina., dV Sosa L; Centro de Microscopia Electrónica, Instituto de Investigaciones en Ciencias de la Salud (INICSA-CONICET), Facultad de Ciencias Medicas, Universidad Nacional de Cordoba, Cordoba, Argentina., Grondona E; Centro de Microscopia Electrónica, Instituto de Investigaciones en Ciencias de la Salud (INICSA-CONICET), Facultad de Ciencias Medicas, Universidad Nacional de Cordoba, Cordoba, Argentina., Mukdsi JH; Centro de Microscopia Electrónica, Instituto de Investigaciones en Ciencias de la Salud (INICSA-CONICET), Facultad de Ciencias Medicas, Universidad Nacional de Cordoba, Cordoba, Argentina., De Paul AL; Centro de Microscopia Electrónica, Instituto de Investigaciones en Ciencias de la Salud (INICSA-CONICET), Facultad de Ciencias Medicas, Universidad Nacional de Cordoba, Cordoba, Argentina., Torres AI; Centro de Microscopia Electrónica, Instituto de Investigaciones en Ciencias de la Salud (INICSA-CONICET), Facultad de Ciencias Medicas, Universidad Nacional de Cordoba, Cordoba, Argentina., Gutierrez S; Centro de Microscopia Electrónica, Instituto de Investigaciones en Ciencias de la Salud (INICSA-CONICET), Facultad de Ciencias Medicas, Universidad Nacional de Cordoba, Cordoba, Argentina. |
| المصدر: | Journal of cellular physiology [J Cell Physiol] 2018 Feb; Vol. 233 (2), pp. 1402-1413. Date of Electronic Publication: 2017 Jun 15. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-Liss Country of Publication: United States NLM ID: 0050222 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-4652 (Electronic) Linking ISSN: 00219541 NLM ISO Abbreviation: J Cell Physiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: New York, NY : Wiley-Liss Original Publication: Philadelphia, Wistar Institute of Anatomy and Biology. |
| مواضيع طبية MeSH: | Cell Proliferation*, Estrogen Receptor beta/*metabolism , Estrous Cycle/*metabolism , Lactotrophs/*enzymology , PTEN Phosphohydrolase/*metabolism , Somatotrophs/*enzymology, Animals ; Cells, Cultured ; Estradiol/metabolism ; Estradiol/pharmacology ; Estrogen Receptor beta/agonists ; Estrogen Receptor beta/genetics ; Estrogen Replacement Therapy ; Female ; G1 Phase ; Lactotrophs/drug effects ; Male ; Nitriles/pharmacology ; Ovariectomy ; Rats, Wistar ; Signal Transduction ; Somatotrophs/drug effects ; Transfection |
| مستخلص: | In this study, we focused on ERβ regulation in the adenohypophysis under different estrogenic milieu, by analyzing whether ER modulates the phosphatase and tensin homolog deleted on chromosome 10 (PTEN) expression and its subcellular localization on anterior pituitary glands from Wistar rats and GH3 lactosomatotroph cells that over-expressed ERβ. ERβ was regulated in a cyclic manner, and underwent dynamic changes throughout the estrous cycle, with decreased ERβ+ cells in estrus and under E2 treatment, but increased in ovariectomized rats. In addition, the ERα/β ratio increased in estrus and under E2 stimulation, but decreased in ovariectomized rats. Double immunofluorescence revealed that lactotroph and somatotroph ERβ+ were significantly decreased in estrus. Also, variations in the PTEN expression was observed, which was diminished with high E2 conditions but augmented with low E2 milieu. The subcellular localization of this phosphatase was cell cycle-dependent, with remarkable changes in the immunostaining pattern: nuclear in arrested pituitary cells but cytoplasmic in stimulated cells, and responding differently to ER agonists, with only DPN being able to increase PTEN expression and retaining it in the nucleus. Finally, ERβ over-expression increased PTEN with a noticeable subcellular redistribution, and with a significant nuclear signal increase in correlation with an increase of cells in G0/G1 phase. These results showed that E2 is able to inhibit ERβ expression and suggests that the tumoral suppressor PTEN might be one of the signaling proteins by which E2, through ERβ, acts to modulate pituitary cell proliferation, thereby adapting endocrine populations in relation with hormonal necessities. (© 2017 Wiley Periodicals, Inc.) |
| فهرسة مساهمة: | Keywords: PTEN; estrogen receptor β; lactotroph; pituitary; somatotroph |
| المشرفين على المادة: | 0 (2,3-bis(4-hydroxyphenyl)-propionitrile) 0 (Estrogen Receptor beta) 0 (Nitriles) 4TI98Z838E (Estradiol) EC 3.1.3.67 (PTEN Phosphohydrolase) EC 3.1.3.67 (Pten protein, rat) |
| تواريخ الأحداث: | Date Created: 20170526 Date Completed: 20171121 Latest Revision: 20220408 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1002/jcp.26025 |
| PMID: | 28542730 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1097-4652 |
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| DOI: | 10.1002/jcp.26025 |