دورية أكاديمية
أعرض في EDS

The nucleus is irreversibly shaped by motion of cell boundaries in cancer and non-cancer cells.

التفاصيل البيبلوغرافية
العنوان: The nucleus is irreversibly shaped by motion of cell boundaries in cancer and non-cancer cells.
المؤلفون: Tocco VJ; Department of Chemical Engineering, University of Florida, Gainesville, Florida., Li Y; Department of Chemical Engineering, University of Florida, Gainesville, Florida., Christopher KG; Department of Chemical Engineering, University of Florida, Gainesville, Florida., Matthews JH; Department of Medicinal Chemistry, Center for Natural Products, Drug Discovery and Development (CNPD3), University of Florida, Gainesville, Florida., Aggarwal V; Department of Chemical Engineering, University of Florida, Gainesville, Florida., Paschall L; Department of Chemical Engineering, University of Florida, Gainesville, Florida., Luesch H; Department of Medicinal Chemistry, Center for Natural Products, Drug Discovery and Development (CNPD3), University of Florida, Gainesville, Florida., Licht JD; Division of Hematology and Oncology, Department of Medicine, University of Florida Health Cancer Center, Gainesville, Florida., Dickinson RB; Department of Chemical Engineering, University of Florida, Gainesville, Florida., Lele TP; Department of Chemical Engineering, University of Florida, Gainesville, Florida.; Department of Anatomy and Cell Biology, University of Florida College of Medicine, Gainesville, Florida.
المصدر: Journal of cellular physiology [J Cell Physiol] 2018 Feb; Vol. 233 (2), pp. 1446-1454. Date of Electronic Publication: 2017 Jul 31.
نوع المنشور: Comparative Study; Journal Article
اللغة: English
بيانات الدورية: Publisher: Wiley-Liss Country of Publication: United States NLM ID: 0050222 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-4652 (Electronic) Linking ISSN: 00219541 NLM ISO Abbreviation: J Cell Physiol Subsets: MEDLINE
أسماء مطبوعة: Publication: New York, NY : Wiley-Liss
Original Publication: Philadelphia, Wistar Institute of Anatomy and Biology.
مواضيع طبية MeSH: Cell Movement* , Cell Nucleus Shape* , Cell Shape*, Breast Neoplasms/*pathology , Cell Nucleus/*pathology , Fibroblasts/*cytology , Stress Fibers/*pathology, Animals ; Cell Line, Tumor ; Energy Transfer ; Female ; Humans ; Mechanotransduction, Cellular ; Mice ; NIH 3T3 Cells ; Stress, Mechanical ; Time Factors
مستخلص: Actomyosin stress fibers impinge on the nucleus and can exert compressive forces on it. These compressive forces have been proposed to elongate nuclei in fibroblasts, and lead to abnormally shaped nuclei in cancer cells. In these models, the elongated or flattened nuclear shape is proposed to store elastic energy. However, we found that deformed shapes of nuclei are unchanged even after removal of the cell with micro-dissection, both for smooth, elongated nuclei in fibroblasts and abnormally shaped nuclei in breast cancer cells. The lack of shape relaxation implies that the nuclear shape in spread cells does not store any elastic energy, and the cellular stresses that deform the nucleus are dissipative, not static. During cell spreading, the deviation of the nucleus from a convex shape increased in MDA-MB-231 cancer cells, but decreased in MCF-10A cells. Tracking changes of nuclear and cellular shape on micropatterned substrata revealed that fibroblast nuclei deform only during deformations in cell shape and only in the direction of nearby moving cell boundaries. We propose that motion of cell boundaries exert a stress on the nucleus, which allows the nucleus to mimic cell shape. The lack of elastic energy in the nuclear shape suggests that nuclear shape changes in cells occur at constant surface area and volume.
(© 2017 Wiley Periodicals, Inc.)
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معلومات مُعتمدة: R01 CA172310 United States CA NCI NIH HHS; R01 EB014869 United States EB NIBIB NIH HHS; R01 GM102486 United States GM NIGMS NIH HHS; U54 CA193419 United States CA NCI NIH HHS
فهرسة مساهمة: Keywords: cell forces; nuclear mechanics; nuclear shape
تواريخ الأحداث: Date Created: 20170526 Date Completed: 20171121 Latest Revision: 20200526
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC5673577
DOI: 10.1002/jcp.26031
PMID: 28542912
قاعدة البيانات: MEDLINE
الوصف
تدمد:1097-4652
DOI:10.1002/jcp.26031