دورية أكاديمية
Comparison of gene expression of Toll-like receptors and cytokines between Piau and Commercial line (Landrace×Large White crossbred) pigs vaccinated against Pasteurella multocida type D.
| العنوان: | Comparison of gene expression of Toll-like receptors and cytokines between Piau and Commercial line (Landrace×Large White crossbred) pigs vaccinated against Pasteurella multocida type D. |
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| المؤلفون: | Sousa KRS; Departament of Animal Science, Universidade Federal de Viçosa, Viçosa, Minas Gerais 36570-000, Brazil. Electronic address: katiene.sousa@ufma.br., Ribeiro AMF; Departament of Animal Science, Universidade Federal de Viçosa, Viçosa, Minas Gerais 36570-000, Brazil., Dantas WMF; Department of Veterinary Medicine, Universidade Federal de Viçosa, Minas Gerais, 36570-000, Brazil., Oliveira LL; Department of Biology, Universidade Federal de Viçosa, Minas Gerais, 36570-000, Brazil., Gasparino E; Departament of Animal Science, Universidade Estadual de Maringá, Maringá, Paraná, 87020-900, Brazil., Guimarães SEF; Departament of Animal Science, Universidade Federal de Viçosa, Viçosa, Minas Gerais 36570-000, Brazil. |
| المصدر: | Research in veterinary science [Res Vet Sci] 2017 Oct; Vol. 114, pp. 273-280. Date of Electronic Publication: 2017 May 22. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: British Veterinary Association Country of Publication: England NLM ID: 0401300 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2661 (Electronic) Linking ISSN: 00345288 NLM ISO Abbreviation: Res Vet Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London : British Veterinary Association Original Publication: Oxford. |
| مواضيع طبية MeSH: | Gene Expression*, Cytokines/*genetics , Pasteurella Infections/*veterinary , Rhinitis, Atrophic/*veterinary , Swine Diseases/*immunology , Toll-Like Receptors/*genetics , Vaccination/*veterinary, Animals ; Cytokines/metabolism ; Female ; Leukocytes, Mononuclear/immunology ; Pasteurella Infections/immunology ; Pasteurella Infections/prevention & control ; Pasteurella multocida/physiology ; Rhinitis, Atrophic/immunology ; Rhinitis, Atrophic/prevention & control ; Sus scrofa/genetics ; Swine ; Swine Diseases/prevention & control ; Toll-Like Receptors/metabolism |
| مستخلص: | We aimed to compare Toll-like receptors (TLR) and cytokines expression in local Piau breed and a Commercial line (Landrace×Large White crossbred) pigs in response to vaccination against Pasteurella multocida type D. Seronegative gilts for Pasteurella multocida type D and Mycoplasma hyopneumoniae were used, from which peripheral blood mononuclear cells (PBMC) were collected in four time points (T0, T1, T2 and T3; before and after each vaccination dose). For bronchoalveolar lavage fluid cells (BALF), we set groups of vaccinated and unvaccinated animals for both genetic groups. Gene expression was evaluated on PBMC and BALF. In PBMC, when we analyzed time points within breeds, significant differences in expression for TLRs and cytokines, except TGFβ, were observed for Commercial animals. For the Piau pigs, only TGFβ showed differential expression. Comparing the expression among genetic groups, the Commercial pigs showed higher expression for TLRs after first vaccination dose, while for IL2, IL6, IL12 and IL13, higher expression was also observed in T3 and IL8 and IL10, in T1 and T3. Still comparing the breeds, the crossbred animals showed higher expression for TNFα in T1 and T2, while for TGFβ only in T2. For gene expression in BALF, vaccinated Commercial pigs showed higher expression of TLR6, TLR10, IL6, IL8, IL10, TNFα and TGFβ genes than vaccinated Piau pigs. The Commercial line pigs showed higher sensitivity to vaccination, while in local Piau breed lower responsiveness, which may partly explain genetic variability in immune response and will let us better understand the tolerance/susceptibility for pasteurellosis. (Copyright © 2017 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Cytokines; Disease resistance; Immune response; Pasteurellosis; Swine |
| المشرفين على المادة: | 0 (Cytokines) 0 (Toll-Like Receptors) |
| تواريخ الأحداث: | Date Created: 20170530 Date Completed: 20180413 Latest Revision: 20181202 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.rvsc.2017.05.019 |
| PMID: | 28554143 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1532-2661 |
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| DOI: | 10.1016/j.rvsc.2017.05.019 |