دورية أكاديمية
أعرض في EDS

Dissemination of bla OXA-370 gene among several Enterobacteriaceae species in Brazil.

التفاصيل البيبلوغرافية
العنوان: Dissemination of bla OXA-370 gene among several Enterobacteriaceae species in Brazil.
المؤلفون: Magagnin CM; Laboratório de Pesquisa em Resistência Bacteriana (LABRESIS) - Centro de Pesquisa Experimental - Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil. cibele-mm@hotmail.com.; Programa de Pós-Graduação em Medicina: Ciências Médicas, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil. cibele-mm@hotmail.com., Rozales FP; Laboratório de Pesquisa em Resistência Bacteriana (LABRESIS) - Centro de Pesquisa Experimental - Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil., Antochevis L; Laboratório de Pesquisa em Resistência Bacteriana (LABRESIS) - Centro de Pesquisa Experimental - Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil., Nunes LS; Laboratório de Pesquisa em Resistência Bacteriana (LABRESIS) - Centro de Pesquisa Experimental - Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.; Universidade Federal do Pampa (UNIPAMPA), Campus Uruguaiana, Brazil., Martins AS; Laboratório de Pesquisa em Resistência Bacteriana (LABRESIS) - Centro de Pesquisa Experimental - Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil., Barth AL; Laboratório de Pesquisa em Resistência Bacteriana (LABRESIS) - Centro de Pesquisa Experimental - Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil., Sampaio JM; Departamento de Análises Clínicas e Toxicológicas-Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, Brazil.; Microbiology Section, Fleury Medicine and Health, São Paulo, São Paulo, Brazil., Zavascki AP; Infectious Diseases Service, Hospital de Clínicas de Porto, Porto Alegre, Brazil.; Department of Internal Medicine, Medical School, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
المصدر: European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology [Eur J Clin Microbiol Infect Dis] 2017 Oct; Vol. 36 (10), pp. 1907-1910. Date of Electronic Publication: 2017 May 30.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer Country of Publication: Germany NLM ID: 8804297 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1435-4373 (Electronic) Linking ISSN: 09349723 NLM ISO Abbreviation: Eur J Clin Microbiol Infect Dis Subsets: MEDLINE
أسماء مطبوعة: Publication: Berlin : Springer
Original Publication: [Wiesbaden, Federal Republic of Germany] : Vieweg, [c1988-
مواضيع طبية MeSH: Carbapenem-Resistant Enterobacteriaceae/*enzymology , Carbapenem-Resistant Enterobacteriaceae/*genetics , beta-Lactamases/*genetics, Brazil ; Carbapenem-Resistant Enterobacteriaceae/classification ; Carbapenem-Resistant Enterobacteriaceae/isolation & purification ; Genotype ; Hospitals ; Humans ; Microbial Sensitivity Tests ; Molecular Typing ; Real-Time Polymerase Chain Reaction ; Sequence Analysis, DNA
مستخلص: OXA-370 is a recently described OXA-48 variant that has only been described in a few Enterobacter spp. and Klebsiella pneumoniae isolates. The purpose of this study is to assess the prevalence of OXA-370-producing isolates in carbapenem-nonsusceptible Enterobacteriaceae recovered from 28 hospitals from Brazil. Real-time PCR was used to determine the presence of bla NDM-1 , bla KPC-2 , bla VIM-type , bla GES-type , bla OXA-48-like , and bla IMP-type genes. A total of 4,451 Enterobacteriaceae were screened. The gene bla OXA-48-like was detected in 74 (2.5%) isolates, mostly of Enterobacter spp. (44.6% E. cloacae and 2.7% E. aerogenes) and Klebsiella spp. (31.1% K. pneumoniae and 6.7% K. oxytoca), followed by Escherichia coli, (6.7%), Morganella morganii, (2.7%), Citrobacter freundii (1.3%), Proteus mirabilis (1.3%), Providencia stuartii (1.3%), and Serratia spp. (1.3%). These isolates were from five hospitals, 67 (90.5%) from the hospital where the bla OXA-370 was first described. Sequencing of bla OXA-48-like was performed in 52 isolates, including E. cloacae, E. aerogenes, K. pneumoniae, K. oxytoca, E. coli, and C. freundii; all presenting 100% identity with bla OXA-370 . PFGE revealed the presence of distinct clones among K. pneumoniae, E. cloacae, K. oxytoca, and E. coli. Susceptibility rates to meropenem, imipenem, and ertapenem among OXA-370-producing isolates were 92.3%, 78.8%, 7.7% respectively; the MIC 50 /MIC 90 were 0.38/2 mg/L and 1/3 mg/L for meropenem and imipenem respectively. Overall, antimicrobial susceptibility analysis suggests that OXA-370 lacks carbapenemase activity. Our study demonstrated that the bla OXA-370 gene is disseminated among several Enterobacteriaceae species and clones, indicating a high potential for dissemination.
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المشرفين على المادة: EC 3.5.2.6 (beta-Lactamases)
تواريخ الأحداث: Date Created: 20170531 Date Completed: 20180625 Latest Revision: 20181113
رمز التحديث: 20221213
DOI: 10.1007/s10096-017-3012-x
PMID: 28555403
قاعدة البيانات: MEDLINE
الوصف
تدمد:1435-4373
DOI:10.1007/s10096-017-3012-x