دورية أكاديمية
Natural lipids-based NLC containing lidocaine: from pre-formulation to in vivo studies.
| العنوان: | Natural lipids-based NLC containing lidocaine: from pre-formulation to in vivo studies. |
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| المؤلفون: | Ribeiro LNM; Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas - UNICAMP, Campinas, - São Paulo, Brazil. Electronic address: nuneslica@gmail.com., Breitkreitz MC; Department of Analytical Chemistry, Institute of Chemistry, UNICAMP, Campinas, - São Paulo, Brazil., Guilherme VA; Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas - UNICAMP, Campinas, - São Paulo, Brazil., da Silva GHR; Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas - UNICAMP, Campinas, - São Paulo, Brazil., Couto VM; Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas - UNICAMP, Campinas, - São Paulo, Brazil., Castro SR; Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas - UNICAMP, Campinas, - São Paulo, Brazil., de Paula BO; Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas - UNICAMP, Campinas, - São Paulo, Brazil., Machado D; Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas - UNICAMP, Campinas, - São Paulo, Brazil., de Paula E; Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas - UNICAMP, Campinas, - São Paulo, Brazil. |
| المصدر: | European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences [Eur J Pharm Sci] 2017 Aug 30; Vol. 106, pp. 102-112. Date of Electronic Publication: 2017 May 27. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science B.V Country of Publication: Netherlands NLM ID: 9317982 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0720 (Electronic) Linking ISSN: 09280987 NLM ISO Abbreviation: Eur J Pharm Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Science B.V Original Publication: Amsterdam ; New York : Elsevier, c1993- |
| مواضيع طبية MeSH: | Drug Carriers/*chemistry , Lidocaine/*pharmacokinetics , Lipids/*chemistry , Poloxamer/*chemistry , Waxes/*chemistry, Animals ; Chemistry, Pharmaceutical/methods ; Drug Liberation ; Drug Stability ; Excipients/chemistry ; Humans ; Lidocaine/administration & dosage ; Lidocaine/chemistry ; Male ; Mice, Inbred BALB C ; Nanoparticles/chemistry ; Particle Size ; Plant Oils/chemistry ; Surface Properties ; Zebrafish |
| مستخلص: | In a nanotechnological approach we have investigated the use of natural lipids in the preparation of nanostructured lipid carriers (NLC). Three different NLC composed of copaiba oil and beeswax, sweet almond oil and shea butter, and sesame oil and cocoa butter as structural matrices were optimized using factorial analysis; Pluronic® 68 and lidocaine (LDC) were used as the colloidal stabilizer and model encapsulated drug, respectively. The optimal formulations were characterized by different techniques (IR-ATR, DSC, and TEM), and their safety and efficacy were also tested. These nanocarriers were able to upload high amounts of the anesthetic with a sustained in vitro release profile for 24h. The physicochemical stability in terms of size (nm), PDI, zeta potential (mV), pH, nanoparticle concentration (particles/mL), and visual inspection was followed during 12months of storage at 25°C. The formulations exhibited excellent structural properties and stability. They proved to be nontoxic in vitro (cell viability tests with Balb/c 3T3 fibroblasts) and significantly improved the in vivo effects of LDC, over the heart rate of zebra fish larvae and in the blockage of sciatic nerve in mice. The results from this study support that the proper combination of natural excipients is promising in DDS, taking advantage of the biocompatibility, low cost, and diversity of lipids. (Copyright © 2017 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Beeswax; Nanostructured lipid carriers; Vegetable fats; Vegetable oils |
| المشرفين على المادة: | 0 (Drug Carriers) 0 (Excipients) 0 (Lipids) 0 (Plant Oils) 0 (Waxes) 106392-12-5 (Poloxamer) 2ZA36H0S2V (beeswax) 66YXD4DKO9 (almond oil) 98PI200987 (Lidocaine) |
| تواريخ الأحداث: | Date Created: 20170601 Date Completed: 20181211 Latest Revision: 20220330 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.ejps.2017.05.060 |
| PMID: | 28558981 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0720 |
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| DOI: | 10.1016/j.ejps.2017.05.060 |