دورية أكاديمية
Urinary exosomes reveal protein signatures in hypertensive patients with albuminuria.
| العنوان: | Urinary exosomes reveal protein signatures in hypertensive patients with albuminuria. |
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| المؤلفون: | Gonzalez-Calero L; Department of Immunology, IIS-Fundacion Jimenez Diaz, REDinREN, Madrid, Spain., Martínez PJ; Department of Immunology, IIS-Fundacion Jimenez Diaz, REDinREN, Madrid, Spain., Martin-Lorenzo M; Department of Immunology, IIS-Fundacion Jimenez Diaz, REDinREN, Madrid, Spain., Baldan-Martin M; Department of Vascular Physiopathology, Hospital Nacional de Paraplejicos SESCAM, Toledo, Spain., Ruiz-Hurtado G; Hypertension Unit, Instituto de Investigación Imas12, Hospital Universitario 12 de Octubre, Madrid, Spain., de la Cuesta F; Department of Vascular Physiopathology, Hospital Nacional de Paraplejicos SESCAM, Toledo, Spain., Calvo E; Ibermutuamur, Madrid, Spain., Segura J; Hypertension Unit, Instituto de Investigación Imas12, Hospital Universitario 12 de Octubre, Madrid, Spain., Lopez JA; Laboratory of Cardiovascular Proteomics CNIC, Madrid, Spain., Vázquez J; Laboratory of Cardiovascular Proteomics CNIC, Madrid, Spain., Barderas MG; Department of Vascular Physiopathology, Hospital Nacional de Paraplejicos SESCAM, Toledo, Spain., Ruilope LM; Hypertension Unit, Instituto de Investigación Imas12, Hospital Universitario 12 de Octubre, Madrid, Spain., Vivanco F; Department of Immunology, IIS-Fundacion Jimenez Diaz, REDinREN, Madrid, Spain.; Department of Biochemistry and Molecular Biology I, Universidad Complutense, Madrid, Spain., Alvarez-Llamas G; Department of Immunology, IIS-Fundacion Jimenez Diaz, REDinREN, Madrid, Spain. |
| المصدر: | Oncotarget [Oncotarget] 2017 Jul 04; Vol. 8 (27), pp. 44217-44231. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Impact Journals Country of Publication: United States NLM ID: 101532965 Publication Model: Print Cited Medium: Internet ISSN: 1949-2553 (Electronic) Linking ISSN: 19492553 NLM ISO Abbreviation: Oncotarget Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Albany, N.Y. : Impact Journals |
| مواضيع طبية MeSH: | Proteome* , Proteomics*/methods, Albuminuria/*metabolism , Exosomes/*metabolism , Hypertension/*metabolism, Aged ; Biomarkers ; Chromatography, Liquid ; Humans ; Hypertension/urine ; Middle Aged ; Protein Interaction Mapping ; Protein Interaction Maps ; ROC Curve ; Renin-Angiotensin System ; Tandem Mass Spectrometry ; Workflow |
| مستخلص: | Albuminuria is an indicator of cardiovascular risk and renal damage in hypertensive individuals. Chronic renin-angiotensin system (RAS) suppression facilitates blood pressure control and prevents development of new-onset-albuminuria. A significant number of patients, however, develop albuminuria despite chronic RAS blockade, and the physiopathological mechanisms are underexplored. Urinary exosomes reflect pathological changes taking place in the kidney. The objective of this work was to examine exosomal protein alterations in hypertensive patients with albuminuria in the presence of chronic RAS suppression, to find novel clues underlying its development. Patients were followed-up for three years and were classified as: a) patients with persistent normoalbuminuria; b) patients developing de novo albuminuria; and c) patients with maintained albuminuria. Exosomal protein alterations between groups were identified by isobaric tag quantitation (iTRAQ). Confirmation was approached by target analysis (SRM). In total, 487 proteins were identified with high confidence. Specifically, 48 proteins showed an altered pattern in response to hypertension and/or albuminuria. Out of them, 21 proteins interact together in three main functional clusters: glycosaminoglycan degradation, coagulation and complement system, and oxidative stress. The identified proteins constitute potential targets for drug development and may help to define therapeutic strategies to evade albuminuria progression in hypertensive patients chronically treated. |
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| فهرسة مساهمة: | Keywords: albuminuria; exosomes; hypertension; proteomics; renin-angiotensin system |
| المشرفين على المادة: | 0 (Biomarkers) 0 (Proteome) |
| تواريخ الأحداث: | Date Created: 20170601 Date Completed: 20180406 Latest Revision: 20181113 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC5546475 |
| DOI: | 10.18632/oncotarget.17787 |
| PMID: | 28562335 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1949-2553 |
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| DOI: | 10.18632/oncotarget.17787 |