دورية أكاديمية
أعرض في EDS

Expression of TLR2, NOD1, and NOD2 and the NLRP3 Inflammasome in Renal Tubular Epithelial Cells of Male versus Female Mice.

التفاصيل البيبلوغرافية
العنوان: Expression of TLR2, NOD1, and NOD2 and the NLRP3 Inflammasome in Renal Tubular Epithelial Cells of Male versus Female Mice.
المؤلفون: DeWolf SE; Division of Nephrology/Hypertension, Department of Medicine, University of California San Diego, La Jolla, CA, USA., Shigeoka AA, Scheinok A, Kasimsetty SG, Welch AK, McKay DB
المصدر: Nephron [Nephron] 2017; Vol. 137 (1), pp. 68-76. Date of Electronic Publication: 2017 Jun 15.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Karger Country of Publication: Switzerland NLM ID: 0331777 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2235-3186 (Electronic) Linking ISSN: 16608151 NLM ISO Abbreviation: Nephron Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel ; New York : Karger
مواضيع طبية MeSH: Inflammasomes/*metabolism , Kidney Tubules/*metabolism , NLR Family, Pyrin Domain-Containing 3 Protein/*metabolism , Nod1 Signaling Adaptor Protein/*metabolism , Nod2 Signaling Adaptor Protein/*metabolism , Toll-Like Receptor 2/*metabolism, Animals ; Caspase 1/metabolism ; Epithelial Cells/immunology ; Epithelial Cells/metabolism ; Female ; Gene Expression ; Inflammasomes/immunology ; Interleukin-6/genetics ; Interleukin-6/metabolism ; Kidney/immunology ; Kidney/injuries ; Kidney/metabolism ; Kidney Tubules/cytology ; Kidney Tubules/immunology ; Male ; Mice ; Mice, Inbred C57BL ; NLR Family, Pyrin Domain-Containing 3 Protein/genetics ; Nod1 Signaling Adaptor Protein/genetics ; Nod2 Signaling Adaptor Protein/genetics ; Receptor-Interacting Protein Serine-Threonine Kinase 2 ; Receptor-Interacting Protein Serine-Threonine Kinases/genetics ; Receptor-Interacting Protein Serine-Threonine Kinases/metabolism ; Receptors, Pattern Recognition/genetics ; Receptors, Pattern Recognition/metabolism ; Reperfusion Injury/immunology ; Reperfusion Injury/metabolism ; Sex Characteristics ; Toll-Like Receptor 2/genetics
مستخلص: Background: Gender-biased outcomes are associated with acute kidney injury (AKI) and human and animal studies have shown that females are preferentially protected from renal ischemia. However, the reason for this is not known. One clue might lie with pattern recognition receptors (PRRs), which are triggers of ischemic injury when ligated by molecules in the ischemic milieu. Several PRR families are expressed by renal tubular epithelial cells (RTEs) and incite cell death signaling and production of pro-inflammatory molecules. Blockade of specific PRRs (e.g., TLR2, NOD1, NOD2, and NLRP3) provides highly significant protection from ischemic RTE injury. As a first step to understand gender-biased outcomes of AKI, we tested whether constitutive gender-based differences exist in expression of these PRRS in RTEs.
Methods: To determine whether PRR expression differences exist, primary RTEs isolated from male and female WT kidneys were examined by FACS, qPCR, and Western Blot for expression of TLR2, NOD1, NOD2, and NLRP3 inflammasome components.
Results: No RTE gender-based differences in TLR2, NOD1, NOD2, NLRP3, or ASC were found. RTEs from female kidneys had approximately half the mRNA, but the same protein concentration of pro-caspase-1 compared to RTEs isolated from male kidneys.
Conclusions: Our findings indicate that intrinsic gender differences in RTE expression of TLR2, NOD1, NOD2, NLRP3, and ASC are not responsible for the gender-biased outcomes observed in ischemia/reperfusion injury. The lower caspase-1 mRNA expression in RTEs from females warrants further exploration of additional upstream signals that might differentially regulate caspase-1 in male vs. female RTEs.
(© 2017 S. Karger AG, Basel.)
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معلومات مُعتمدة: R01 DK075718 United States DK NIDDK NIH HHS; R01 DK091136 United States DK NIDDK NIH HHS; R01 DK113162 United States DK NIDDK NIH HHS
فهرسة مساهمة: Keywords: Acute kidney injury; Gender differences; NOD-like receptors; Pattern recognition receptors; Toll-like receptors
المشرفين على المادة: 0 (Inflammasomes)
0 (Interleukin-6)
0 (NLR Family, Pyrin Domain-Containing 3 Protein)
0 (Nlrp3 protein, mouse)
0 (Nod1 Signaling Adaptor Protein)
0 (Nod1 protein, mouse)
0 (Nod2 Signaling Adaptor Protein)
0 (Nod2 protein, mouse)
0 (Receptors, Pattern Recognition)
0 (Tlr2 protein, mouse)
0 (Toll-Like Receptor 2)
0 (interleukin-6, mouse)
EC 2.7.11.1 (Receptor-Interacting Protein Serine-Threonine Kinase 2)
EC 2.7.11.1 (Receptor-Interacting Protein Serine-Threonine Kinases)
EC 2.7.11.1 (Ripk2 protein, mouse)
EC 3.4.22.36 (Caspase 1)
تواريخ الأحداث: Date Created: 20170615 Date Completed: 20180521 Latest Revision: 20230721
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC5599100
DOI: 10.1159/000456016
PMID: 28614830
قاعدة البيانات: MEDLINE
الوصف
تدمد:2235-3186
DOI:10.1159/000456016