دورية أكاديمية
أعرض في EDS

Clinical course and core variability in HBV infected patients without detectable anti-HBc antibodies.

التفاصيل البيبلوغرافية
العنوان: Clinical course and core variability in HBV infected patients without detectable anti-HBc antibodies.
المؤلفون: Anastasiou OE; Institute of Virology, University Hospital Essen, University Duisburg-Essen, Germany; Department of Gastroenterology and Hepatology, University Hospital of Essen, Germany. Electronic address: olympia_anastasiou@yahoo.com., Widera M; Institute of Virology, University Hospital Essen, University Duisburg-Essen, Germany., Verheyen J; Institute of Virology, University Hospital Essen, University Duisburg-Essen, Germany., Korth J; Institute of Virology, University Hospital Essen, University Duisburg-Essen, Germany., Gerken G; Department of Gastroenterology and Hepatology, University Hospital of Essen, Germany., Helfritz FA; Department of General, Visceral and Transplantation Surgery, University Hospital of Essen, Germany., Canbay A; Department of Gastroenterology and Hepatology, University Hospital of Essen, Germany., Wedemeyer H; Department of Gastroenterology and Hepatology, Hannover Medical School, Germany; German Center for Infection Research, DZIF, Germany., Ciesek S; Institute of Virology, University Hospital Essen, University Duisburg-Essen, Germany; German Center for Infection Research, DZIF, Germany.
المصدر: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology [J Clin Virol] 2017 Aug; Vol. 93, pp. 46-52. Date of Electronic Publication: 2017 Jun 08.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 9815671 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-5967 (Electronic) Linking ISSN: 13866532 NLM ISO Abbreviation: J Clin Virol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam : Elsevier Science, c1998-
مواضيع طبية MeSH: DNA, Viral/*blood , Hepatitis B/*blood , Hepatitis B Antibodies/*blood , Hepatitis B Core Antigens/*immunology , Hepatitis B virus/*genetics, Adult ; Aged ; Female ; Genetic Variation ; Hepatitis B/diagnosis ; Hepatitis B/immunology ; Hepatitis B/virology ; Hepatitis B Core Antigens/genetics ; Hepatitis B virus/immunology ; High-Throughput Nucleotide Sequencing ; Humans ; Male ; Middle Aged ; Molecular Diagnostic Techniques ; Sequence Analysis, DNA ; Viral Load ; Young Adult
مستخلص: Background: The presence of anti-HBc antibodies indicates direct encounter of the immune system with hepatitis B virus (HBV).
Objectives: Aim of our study was to seek for anti-HBc negative but HBV replicating patients and analyze their clinical course and preconditions.
Study Design: From 1568 HBV-DNA positive patients, 29 patients (1.85%) tested negative for anti-HBc. The absence of anti-HBc could be confirmed in 19 patients using an alternative assay. In 16 of 19 cases, a partial or full HBV genome analysis was performed with NGS sequencing to evaluate if specific mutations were associated with anti-HBc absence. As a control group samples from 32 matched HBV infected patients with detectable anti-HBc were sequenced.
Results: Patients with detectable HBV-DNA and sequenced HBV core region in the confirmed absence of anti-HBc were diagnosed with acute HBV infection (n=3), HBV reactivation (n=9) and chronic hepatitis B (n=4). Most patients (12/16) were immunosuppressed: 3/16 patients had an HIV coinfection, 7/16 patients suffered from a malignant disease and 4/16 patients underwent solid organ transplantation (from which 2/4 had a malignant disease). Compared to the control cohort, HBV variants from anti-HBc negative patients showed less variability in the core region.
Conclusions: In the absence of anti-HBc, HBV-DNA was most often found in immunocompromised hosts. Distinct mutations or deletions in the core region did not explain anti-HBc negativity. It would be advisable not to rely only on a single result of anti-HBc negativity to exclude HBV infection in immunocompromised hosts, but to measure anti-HBc repeatedly or with different methods.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: HBV; NGS; anti-HBc; core antigen; hepatitis B; immunosuppression
المشرفين على المادة: 0 (DNA, Viral)
0 (Hepatitis B Antibodies)
0 (Hepatitis B Core Antigens)
تواريخ الأحداث: Date Created: 20170617 Date Completed: 20180219 Latest Revision: 20220321
رمز التحديث: 20240628
DOI: 10.1016/j.jcv.2017.06.001
PMID: 28622640
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-5967
DOI:10.1016/j.jcv.2017.06.001