دورية أكاديمية
أعرض في EDS

Long-term imaging of cellular forces with high precision by elastic resonator interference stress microscopy.

التفاصيل البيبلوغرافية
العنوان: Long-term imaging of cellular forces with high precision by elastic resonator interference stress microscopy.
المؤلفون: Kronenberg NM; SUPA, School of Physics and Astronomy, University of St Andrews, St Andrews KY16 9SS, UK., Liehm P; SUPA, School of Physics and Astronomy, University of St Andrews, St Andrews KY16 9SS, UK., Steude A; SUPA, School of Physics and Astronomy, University of St Andrews, St Andrews KY16 9SS, UK., Knipper JA; Institute of Immunology and Infection Research, University of Edinburgh, Edinburgh EH9 3FL, UK., Borger JG; Institute of Immunology and Infection Research, University of Edinburgh, Edinburgh EH9 3FL, UK., Scarcelli G; Fischell Department of Bioengineering, University of Maryland, College Park, Maryland 20742, USA., Franze K; Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 3DY, UK., Powis SJ; School of Medicine, University of St Andrews, St Andrews KY16 9TF, UK., Gather MC; SUPA, School of Physics and Astronomy, University of St Andrews, St Andrews KY16 9SS, UK.
المصدر: Nature cell biology [Nat Cell Biol] 2017 Jul; Vol. 19 (7), pp. 864-872. Date of Electronic Publication: 2017 Jun 19.
نوع المنشور: Journal Article; Video-Audio Media
اللغة: English
بيانات الدورية: Publisher: Macmillan Magazines Ltd Country of Publication: England NLM ID: 100890575 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1476-4679 (Electronic) Linking ISSN: 14657392 NLM ISO Abbreviation: Nat Cell Biol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Macmillan Magazines Ltd., [1999-
مواضيع طبية MeSH: Cell Movement*, Dictyostelium/*physiology , Fibroblasts/*physiology , Macrophages/*physiology , Microscopy, Interference/*methods , Podosomes/*physiology , T-Lymphocytes/*physiology, 3T3 Cells ; Animals ; Biomechanical Phenomena ; Cell Adhesion ; Dictyostelium/metabolism ; Elasticity ; Extracellular Matrix/metabolism ; Fibroblasts/metabolism ; Humans ; Macrophages/metabolism ; Mice ; Mice, Inbred BALB C ; Mice, Transgenic ; Microscopy, Video ; Podosomes/metabolism ; Stress, Mechanical ; T-Lymphocytes/metabolism ; Time Factors ; Time-Lapse Imaging
مستخلص: Cellular forces are crucial for many biological processes but current methods to image them have limitations with respect to data analysis, resolution and throughput. Here, we present a robust approach to measure mechanical cell-substrate interactions in diverse biological systems by interferometrically detecting deformations of an elastic micro-cavity. Elastic resonator interference stress microscopy (ERISM) yields stress maps with exceptional precision and large dynamic range (2 nm displacement resolution over a >1 μm range, translating into 1 pN force sensitivity). This enables investigation of minute vertical stresses (<1 Pa) involved in podosome protrusion, protein-specific cell-substrate interaction and amoeboid migration through spatial confinement in real time. ERISM requires no zero-force reference and avoids phototoxic effects, which facilitates force monitoring over multiple days and at high frame rates and eliminates the need to detach cells after measurements. This allows observation of slow processes such as differentiation and further investigation of cells, for example, by immunostaining.
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معلومات مُعتمدة: G1100116 United Kingdom MRC_ Medical Research Council; G1100312 United Kingdom MRC_ Medical Research Council
تواريخ الأحداث: Date Created: 20170620 Date Completed: 20170913 Latest Revision: 20220129
رمز التحديث: 20231215
DOI: 10.1038/ncb3561
PMID: 28628084
قاعدة البيانات: MEDLINE
الوصف
تدمد:1476-4679
DOI:10.1038/ncb3561