دورية أكاديمية
أعرض في EDS

Oocyte polarized light microscopy, assay of specific follicular fluid metabolites, and gene expression in cumulus cells as different approaches to predict fertilization efficiency after ICSI.

التفاصيل البيبلوغرافية
العنوان: Oocyte polarized light microscopy, assay of specific follicular fluid metabolites, and gene expression in cumulus cells as different approaches to predict fertilization efficiency after ICSI.
المؤلفون: Revelli A; Chair of Gynecology and Obstetrics 1, Physiopathology of Reproduction and IVF Unit, Department of Surgical Sciences, S. Anna Hospital, University of Torino, Torino, Italy. alberto.revelli@unito.it., Canosa S; Chair of Gynecology and Obstetrics 1, Physiopathology of Reproduction and IVF Unit, Department of Surgical Sciences, S. Anna Hospital, University of Torino, Torino, Italy., Bergandi L; Department of Oncology, University of Torino, Torino, Italy., Skorokhod OA; Department of Oncology, University of Torino, Torino, Italy., Biasoni V; Chair of Gynecology and Obstetrics 1, Physiopathology of Reproduction and IVF Unit, Department of Surgical Sciences, S. Anna Hospital, University of Torino, Torino, Italy., Carosso A; Chair of Gynecology and Obstetrics 1, Physiopathology of Reproduction and IVF Unit, Department of Surgical Sciences, S. Anna Hospital, University of Torino, Torino, Italy., Bertagna A; Endocrinology Laboratory, Department of Internal Medicine, University of Torino, Torino, Italy., Maule M; Department of Medical Sciences, Cancer Epidemiology Unit, University of Torino, Torino, Italy., Aldieri E; Department of Oncology, University of Torino, Torino, Italy., D'Eufemia MD; Department of Woman, Child, General and Special Surgery, Second University of Napoli, Naples, Italy., Evangelista F; Chair of Gynecology and Obstetrics 1, Physiopathology of Reproduction and IVF Unit, Department of Surgical Sciences, S. Anna Hospital, University of Torino, Torino, Italy., Colacurci N; Department of Woman, Child, General and Special Surgery, Second University of Napoli, Naples, Italy., Benedetto C; Chair of Gynecology and Obstetrics 1, Physiopathology of Reproduction and IVF Unit, Department of Surgical Sciences, S. Anna Hospital, University of Torino, Torino, Italy.
المصدر: Reproductive biology and endocrinology : RB&E [Reprod Biol Endocrinol] 2017 Jun 23; Vol. 15 (1), pp. 47. Date of Electronic Publication: 2017 Jun 23.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101153627 Publication Model: Electronic Cited Medium: Internet ISSN: 1477-7827 (Electronic) Linking ISSN: 14777827 NLM ISO Abbreviation: Reprod Biol Endocrinol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, 2003-
مواضيع طبية MeSH: Fertilization* , Gene Expression*, Cumulus Cells/*metabolism , Follicular Fluid/*metabolism , Oocytes/*cytology , Sperm Injections, Intracytoplasmic/*methods, Adult ; Anti-Mullerian Hormone/genetics ; Anti-Mullerian Hormone/metabolism ; Embryonic Development ; Female ; Humans ; Microscopy, Polarization/methods ; Oocyte Retrieval/methods ; Receptors, Peptide/genetics ; Receptors, Peptide/metabolism ; Receptors, Transforming Growth Factor beta/genetics ; Receptors, Transforming Growth Factor beta/metabolism
مستخلص: Background: The complex relationship between oocyte morphology, specific follicular fluid metabolites, gene expression in cumulus granulosa cells, and oocyte competence toward fertilization and embryo development still needs further clarification.
Methods: Forty-six oocytes retrieved from the largest pre-ovulatory follicle of patients undergoing intra-cytoplasmic sperm injection (ICSI) were considered assessing: (a) oocyte morphological characteristics at polarized light microscopy (PLM), (b) specific follicular fluid (FF) metabolites previously suggested to influence oocyte competence (AMH, markers of redox status and of cytotoxicity), (c) transcription of AMH and AMH type II receptor genes in cumulus cells. Data were analyzed using mono-parametric tests and multivariable logistic analysis in order to correlate morphological and biochemical data with fertilization.
Results: Comparing normally fertilized oocytes (n = 29, F group) with unfertilized (n = 17, nF group) we observed that: (a) the meiotic spindle area and major axis were significantly higher in nF group and in fertilized oocytes undergoing an early embryo development arrest; (b) AMH level in FF was comparable in F and nF groups; (c) the FF of nF group contained significantly higher levels of cytotoxicity (lactate dehydrogenase) and oxidative stress (Cu,Zn-superoxide dismutase, catalase, 4-hydroxynonenal-protein conjugates) markers; (d) cumulus cells of nF group showed significantly higher AMH receptor type II gene expression.
Conclusions: Taken together, these observations suggest that an excessive cytotoxicity level can alter AMH signal transduction within cumulus cells, in turn leading to partial inhibition of aromatase activity, altered cytoplasmic maturation and increased oxidative stress, factors able to impair oocyte fertilization competence and embryo growth.
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فهرسة مساهمة: Keywords: AMH receptor type II; Amh; Cumulus cells; Fertilization; Follicular fluid; Oocyte; Oxidative stress; Polarized light microscopy
المشرفين على المادة: 0 (Receptors, Peptide)
0 (Receptors, Transforming Growth Factor beta)
0 (anti-Mullerian hormone receptor)
80497-65-0 (Anti-Mullerian Hormone)
تواريخ الأحداث: Date Created: 20170625 Date Completed: 20180417 Latest Revision: 20220409
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC5481970
DOI: 10.1186/s12958-017-0265-2
PMID: 28645283
قاعدة البيانات: MEDLINE
الوصف
تدمد:1477-7827
DOI:10.1186/s12958-017-0265-2