دورية أكاديمية
Global Monitoring of the Mammalian Lipidome by Quantitative Shotgun Lipidomics.
| العنوان: | Global Monitoring of the Mammalian Lipidome by Quantitative Shotgun Lipidomics. |
|---|---|
| المؤلفون: | Nielsen IØ; Cell Death and Metabolism Unit, Center for Autophagy, Recycling and Disease, Danish Cancer Society Research Center, Copenhagen, 2100, Denmark., Maeda K; Cell Death and Metabolism Unit, Center for Autophagy, Recycling and Disease, Danish Cancer Society Research Center, Copenhagen, 2100, Denmark., Bilgin M; Cell Death and Metabolism Unit, Center for Autophagy, Recycling and Disease, Danish Cancer Society Research Center, Copenhagen, 2100, Denmark. mesutb@cancer.dk. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2017; Vol. 1609, pp. 123-139. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Lipid Metabolism* , Metabolome*, Lipids/*chemistry , Metabolomics/*methods, Animals ; Chemical Fractionation ; Humans ; Mammals ; Spectrometry, Mass, Electrospray Ionization/methods ; Workflow |
| مستخلص: | The emerging field of lipidomics presents the systems biology approach to identify and quantify the full lipid repertoire of cells, tissues, and organisms. The importance of the lipidome is demonstrated by a number of biological studies on dysregulation of lipid metabolism in human diseases such as cancer, diabetes, and neurodegenerative diseases. Exploring changes and regulations in the huge networks of lipids and their metabolic pathways requires a lipidomics methodology: Advanced mass spectrometry that resolves the complexity of the lipidome. Here, we report a comprehensive protocol of quantitative shotgun lipidomics that enables identification and quantification of hundreds of molecular lipid species, covering a wide range of lipid classes, extracted from cultured mammalian cells. |
| فهرسة مساهمة: | Keywords: Global monitoring; Lipid extraction; Lipidomics; Mammalian cells; Mass spectrometry; Quantification; Shotgun lipidomics; Systems biology |
| المشرفين على المادة: | 0 (Lipids) |
| تواريخ الأحداث: | Date Created: 20170630 Date Completed: 20180326 Latest Revision: 20180625 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1007/978-1-4939-6996-8_12 |
| PMID: | 28660579 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-6029 |
|---|---|
| DOI: | 10.1007/978-1-4939-6996-8_12 |