دورية أكاديمية
[Effect of Herba Scutellariae Barbatae flavonoids in delaying aging of Caenorhabditis elegans and human umbilical vein endothelial cells in vitro].
| العنوان: | [Effect of Herba Scutellariae Barbatae flavonoids in delaying aging of Caenorhabditis elegans and human umbilical vein endothelial cells in vitro]. |
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| المؤلفون: | Zhou W; Department of Pharmacy, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. E-mail: zhouwei-327@hotmail.com., Lin JM, Wang SL, Lin HZ |
| المصدر: | Nan fang yi ke da xue xue bao = Journal of Southern Medical University [Nan Fang Yi Ke Da Xue Xue Bao] 2017 Jun 20; Vol. 37 (6), pp. 821-826. |
| نوع المنشور: | Journal Article |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Nanfang yi ke da xue xue bao bian ji bu Country of Publication: China NLM ID: 101266132 Publication Model: Print Cited Medium: Print ISSN: 1673-4254 (Print) Linking ISSN: 16734254 NLM ISO Abbreviation: Nan Fang Yi Ke Da Xue Xue Bao Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Guangzhou : Nanfang yi ke da xue xue bao bian ji bu, 2005- |
| مواضيع طبية MeSH: | Aging*, Caenorhabditis elegans/*drug effects , Cellular Senescence/*drug effects , Flavonoids/*pharmacology , Human Umbilical Vein Endothelial Cells/*drug effects , Plant Extracts/*pharmacology, Animals ; Antioxidants/metabolism ; Caenorhabditis elegans Proteins/genetics ; Cells, Cultured ; Forkhead Transcription Factors/genetics ; Humans ; Longevity ; Oxidative Stress ; Scutellaria ; Sirtuin 1/genetics |
| مستخلص: | Objective: To explore the effects of Herba Scutellariae Barbatae flavonoids (HF) in delaying aging of Caenorhabditis elegans and human umbilical vein endothelial cells (HUVECs) in vitro. Methods: The effects of 30 or 50 mg/L of HF on nematode life span, reproductive capacity, oxidative stress, and antioxidant enzyme activity of C. elegans were assessed, and the effects of HF on the expressions of the genes encoding antioxidant enzymes and the aging-related genes were analyzed using real-time RT-PCR in both C. elegans and cultured HUVECs. Results Compared with the blank control group, C. elegans with HF treatment showed significantly improved mean and maximum lifespan with a prolonged mean lifespan under acute heat stress at 35 degrees celsius;. HF treatment did not impair the reproductive capacity or cause significant changes in the offspring number of C. elegans. In addition, HF enhanced SOD and CAT activity and up-regulated the expression of daf-16 and sir-2.1 (SIRT1) genes in C. elegans and HUVECs. Conclusions: HF may delay aging of C. elegans and enhance their resistance to acute heat stress without damaging their reproductive capacity possibly by up-regulating the activity of antioxidant enzymes and expressions of antioxidant genes. HF also may protect endothelial cells against oxidative damage. |
| References: | Ann N Y Acad Sci. 2007 Apr;1100:530-42. (PMID: 17460219) Sheng Li Xue Bao. 2014 Oct 25;66(5):575-82. (PMID: 25332003) Arterioscler Thromb Vasc Biol. 2008 Apr;28(4):622-8. (PMID: 18162611) Prog Mol Biol Transl Sci. 2017;146:203-241. (PMID: 28253986) Aging Cell. 2013 Oct;12(5):772-83. (PMID: 23714110) J Med Food. 2017 May;20(5):526-534. (PMID: 28294699) Chem Biol Interact. 2006 Oct 27;163(1-2):29-37. (PMID: 16737690) Cell Stress Chaperones. 2014 Mar;19(2):295-8. (PMID: 24052229) FEBS Lett. 2011 Apr 6;585(7):986-94. (PMID: 21130086) Int Immunopharmacol. 2004 Mar;4(3):447-54. (PMID: 15072097) Life Sci. 2016 Jul 15;157:200-207. (PMID: 26775564) J Ethnopharmacol. 2016 Apr 22;182:170-80. (PMID: 26875645) Free Radic Biol Med. 2017 Jul;108:750-759. (PMID: 28431961) Biochem Biophys Res Commun. 2015 Dec 25;468(4):843-9. (PMID: 26592451) Cell. 2006 Jun 16;125(6):1165-77. (PMID: 16777605) Biochem J. 2017 Jan 15;474(2):301-315. (PMID: 28062841) Comp Biochem Physiol B Biochem Mol Biol. 2008 Feb;149(2):314-23. (PMID: 18024103) |
| المشرفين على المادة: | 0 (Antioxidants) 0 (Caenorhabditis elegans Proteins) 0 (Flavonoids) 0 (Forkhead Transcription Factors) 0 (Plant Extracts) 0 (Scutellaria barbata extract) 0 (daf-16 protein, C elegans) EC 3.5.1.- (Sirtuin 1) |
| تواريخ الأحداث: | Date Created: 20170704 Date Completed: 20181121 Latest Revision: 20230916 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC6744147 |
| PMID: | 28669960 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1673-4254 |
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