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أعرض في EDS

Reconstruction of the mouse extrahepatic biliary tree using primary human extrahepatic cholangiocyte organoids.

التفاصيل البيبلوغرافية
العنوان: Reconstruction of the mouse extrahepatic biliary tree using primary human extrahepatic cholangiocyte organoids.
المؤلفون: Sampaziotis F; Wellcome Trust-Medical Research Council Stem Cell Institute, Cambridge Stem Cell Institute, Anne McLaren Laboratory, University of Cambridge, Cambridge, UK.; Department of Surgery, University of Cambridge and NIHR Cambridge Biomedical Research Centre, Cambridge, UK.; Department of Hepatology, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK., Justin AW; Department of Engineering, University of Cambridge, Cambridge, UK., Tysoe OC; Wellcome Trust-Medical Research Council Stem Cell Institute, Cambridge Stem Cell Institute, Anne McLaren Laboratory, University of Cambridge, Cambridge, UK.; Department of Surgery, University of Cambridge and NIHR Cambridge Biomedical Research Centre, Cambridge, UK., Sawiak S; Department of Clinical Neurosciences, University of Cambridge, Cambridge, UK., Godfrey EM; Department of Radiology, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK., Upponi SS; Department of Radiology, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK., Gieseck RL 3rd; Immunopathogenesis Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, US National Institutes of Health, Bethesda, Maryland, USA., de Brito MC; Wellcome Trust-Medical Research Council Stem Cell Institute, Cambridge Stem Cell Institute, Anne McLaren Laboratory, University of Cambridge, Cambridge, UK.; Department of Surgery, University of Cambridge and NIHR Cambridge Biomedical Research Centre, Cambridge, UK., Berntsen NL; Norwegian PSC Research Center, Department of Transplantation Medicine, Division of Surgery, Inflammatory Diseases and Transplantation, Oslo University Hospital, Rikshospitalet, Oslo, Norway., Gómez-Vázquez MJ; Cambridge Genomic Services, Department of Pathology, University of Cambridge, Cambridge, UK., Ortmann D; Wellcome Trust-Medical Research Council Stem Cell Institute, Cambridge Stem Cell Institute, Anne McLaren Laboratory, University of Cambridge, Cambridge, UK.; Department of Surgery, University of Cambridge and NIHR Cambridge Biomedical Research Centre, Cambridge, UK., Yiangou L; Wellcome Trust-Medical Research Council Stem Cell Institute, Cambridge Stem Cell Institute, Anne McLaren Laboratory, University of Cambridge, Cambridge, UK.; Department of Medicine, School of Clinical Medicine, University of Cambridge, Cambridge, UK.; Division of Cardiovascular Medicine, University of Cambridge, Cambridge, UK., Ross A; Wellcome Trust-Medical Research Council Stem Cell Institute, Cambridge Stem Cell Institute, Anne McLaren Laboratory, University of Cambridge, Cambridge, UK.; University Department of Paediatrics, University of Cambridge, Cambridge, UK.; Department of Paediatric Gastroenterology, Hepatology and Nutrition, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK., Bargehr J; Wellcome Trust-Medical Research Council Stem Cell Institute, Cambridge Stem Cell Institute, Anne McLaren Laboratory, University of Cambridge, Cambridge, UK.; Department of Medicine, School of Clinical Medicine, University of Cambridge, Cambridge, UK.; Division of Cardiovascular Medicine, University of Cambridge, Cambridge, UK.; Division of Cardiovascular Medicine, University of Cambridge, Cambridge, UK., Bertero A; Wellcome Trust-Medical Research Council Stem Cell Institute, Cambridge Stem Cell Institute, Anne McLaren Laboratory, University of Cambridge, Cambridge, UK.; Department of Surgery, University of Cambridge and NIHR Cambridge Biomedical Research Centre, Cambridge, UK., Zonneveld MCF; Wellcome Trust-Medical Research Council Stem Cell Institute, Cambridge Stem Cell Institute, Anne McLaren Laboratory, University of Cambridge, Cambridge, UK., Pedersen MT; Biotech Research and Innovation Centre (BRIC), University of Copenhagen, Copenhagen, Denmark., Pawlowski M; Wellcome Trust-Medical Research Council Stem Cell Institute, Cambridge Stem Cell Institute, Anne McLaren Laboratory, University of Cambridge, Cambridge, UK., Valestrand L; Norwegian PSC Research Center, Department of Transplantation Medicine, Division of Surgery, Inflammatory Diseases and Transplantation, Oslo University Hospital, Rikshospitalet, Oslo, Norway., Madrigal P; Wellcome Trust-Medical Research Council Stem Cell Institute, Cambridge Stem Cell Institute, Anne McLaren Laboratory, University of Cambridge, Cambridge, UK.; Wellcome Trust Sanger Institute, Hinxton, UK., Georgakopoulos N; Department of Surgery, University of Cambridge and NIHR Cambridge Biomedical Research Centre, Cambridge, UK., Pirmadjid N; Department of Surgery, University of Cambridge and NIHR Cambridge Biomedical Research Centre, Cambridge, UK., Skeldon GM; School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh, UK.; Department of Biomedical Engineering, University of Strathclyde, Glasgow, UK., Casey J; Department of Surgery, University of Edinburgh, Edinburgh Royal Infirmary, Edinburgh, UK., Shu W; School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh, UK.; Department of Biomedical Engineering, University of Strathclyde, Glasgow, UK., Materek PM; NIHR Cambridge Biomedical Centre (BRC) hIPSCs Core Facility, Addenbrooke's Hospital, University of Cambridge, Cambridge, UK., Snijders KE; Wellcome Trust-Medical Research Council Stem Cell Institute, Cambridge Stem Cell Institute, Anne McLaren Laboratory, University of Cambridge, Cambridge, UK., Brown SE; Wellcome Trust-Medical Research Council Stem Cell Institute, Cambridge Stem Cell Institute, Anne McLaren Laboratory, University of Cambridge, Cambridge, UK.; Department of Surgery, University of Cambridge and NIHR Cambridge Biomedical Research Centre, Cambridge, UK., Rimland CA; Wellcome Trust-Medical Research Council Stem Cell Institute, Cambridge Stem Cell Institute, Anne McLaren Laboratory, University of Cambridge, Cambridge, UK.; Department of Surgery, University of Cambridge and NIHR Cambridge Biomedical Research Centre, Cambridge, UK.; Immunopathogenesis Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, US National Institutes of Health, Bethesda, Maryland, USA.; University of North Carolina, Chapel Hill, School of Medicine, Chapel Hill, North Carolina, USA., Simonic I; Medical Genetics Laboratories, Cambridge University Hospitals NHS Trust, Cambridge, UK., Davies SE; Department of Histopathology, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK., Jensen KB; Department of Paediatric Gastroenterology, Hepatology and Nutrition, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK., Zilbauer M; Wellcome Trust-Medical Research Council Stem Cell Institute, Cambridge Stem Cell Institute, Anne McLaren Laboratory, University of Cambridge, Cambridge, UK.; Division of Cardiovascular Medicine, University of Cambridge, Cambridge, UK., Gelson WTH; Department of Hepatology, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK., Alexander GJ; Department of Medicine, School of Clinical Medicine, University of Cambridge, Cambridge, UK.; Division of Cardiovascular Medicine, University of Cambridge, Cambridge, UK., Sinha S; Wellcome Trust-Medical Research Council Stem Cell Institute, Cambridge Stem Cell Institute, Anne McLaren Laboratory, University of Cambridge, Cambridge, UK.; University Department of Paediatrics, University of Cambridge, Cambridge, UK., Hannan NRF; Center for Biomolecular Sciences, University of Nottingham, Nottingham, UK.; Nottingham Digestive Diseases Centre, NIHR Nottingham Biomedical Research Centre at the Nottingham University Hospitals NHS Trust and University of Nottingham, Nottingham, UK., Wynn TA; Immunopathogenesis Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, US National Institutes of Health, Bethesda, Maryland, USA., Karlsen TH; Norwegian PSC Research Center, Department of Transplantation Medicine, Division of Surgery, Inflammatory Diseases and Transplantation, Oslo University Hospital, Rikshospitalet, Oslo, Norway., Melum E; Norwegian PSC Research Center, Department of Transplantation Medicine, Division of Surgery, Inflammatory Diseases and Transplantation, Oslo University Hospital, Rikshospitalet, Oslo, Norway., Markaki AE; Department of Engineering, University of Cambridge, Cambridge, UK., Saeb-Parsy K; Department of Surgery, University of Cambridge and NIHR Cambridge Biomedical Research Centre, Cambridge, UK., Vallier L; Wellcome Trust-Medical Research Council Stem Cell Institute, Cambridge Stem Cell Institute, Anne McLaren Laboratory, University of Cambridge, Cambridge, UK.; Department of Surgery, University of Cambridge and NIHR Cambridge Biomedical Research Centre, Cambridge, UK.; Wellcome Trust Sanger Institute, Hinxton, UK.
المصدر: Nature medicine [Nat Med] 2017 Aug; Vol. 23 (8), pp. 954-963. Date of Electronic Publication: 2017 Jul 03.
نوع المنشور: Journal Article; Video-Audio Media
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Company Country of Publication: United States NLM ID: 9502015 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1546-170X (Electronic) Linking ISSN: 10788956 NLM ISO Abbreviation: Nat Med Subsets: MEDLINE
أسماء مطبوعة: Publication: New York Ny : Nature Publishing Company
Original Publication: New York, NY : Nature Pub. Co., [1995-
مواضيع طبية MeSH: Bile Ducts, Extrahepatic/*physiology , Epithelial Cells/*cytology , Gallbladder/*physiology , Organoids/*physiology , Regeneration/*physiology , Tissue Engineering/*methods, Animals ; Bile Ducts, Extrahepatic/cytology ; Bile Ducts, Extrahepatic/injuries ; Biliary Tract/cytology ; Biliary Tract/injuries ; Biliary Tract/physiology ; Cell Transplantation ; Cystic Fibrosis Transmembrane Conductance Regulator/metabolism ; Epithelial Cells/drug effects ; Epithelial Cells/metabolism ; Gallbladder/injuries ; Humans ; In Vitro Techniques ; Keratin-19/metabolism ; Keratin-7/metabolism ; Mice ; Organoids/cytology ; Organoids/drug effects ; Organoids/metabolism ; Secretin/pharmacology ; Somatostatin/pharmacology ; Tissue Scaffolds ; gamma-Glutamyltransferase/metabolism
مستخلص: The treatment of common bile duct (CBD) disorders, such as biliary atresia or ischemic strictures, is restricted by the lack of biliary tissue from healthy donors suitable for surgical reconstruction. Here we report a new method for the isolation and propagation of human cholangiocytes from the extrahepatic biliary tree in the form of extrahepatic cholangiocyte organoids (ECOs) for regenerative medicine applications. The resulting ECOs closely resemble primary cholangiocytes in terms of their transcriptomic profile and functional properties. We explore the regenerative potential of these organoids in vivo and demonstrate that ECOs self-organize into bile duct-like tubes expressing biliary markers following transplantation under the kidney capsule of immunocompromised mice. In addition, when seeded on biodegradable scaffolds, ECOs form tissue-like structures retaining biliary characteristics. The resulting bioengineered tissue can reconstruct the gallbladder wall and repair the biliary epithelium following transplantation into a mouse model of injury. Furthermore, bioengineered artificial ducts can replace the native CBD, with no evidence of cholestasis or occlusion of the lumen. In conclusion, ECOs can successfully reconstruct the biliary tree, providing proof of principle for organ regeneration using human primary cholangiocytes expanded in vitro.
التعليقات: Comment in: Nat Rev Gastroenterol Hepatol. 2017 Sep;14(9):504-505. (PMID: 28698664)
Comment in: Nature. 2017 Jul 12;547(7662):171-172. (PMID: 28703183)
معلومات مُعتمدة: MR/L016761/1 United Kingdom MRC_ Medical Research Council; FS/13/65/30441 United Kingdom BHF_ British Heart Foundation; MC_UU_12012/5 United Kingdom MRC_ Medical Research Council; FS/13/29/30024 United Kingdom BHF_ British Heart Foundation; MC_PC_12009 United Kingdom MRC_ Medical Research Council
المشرفين على المادة: 0 (Keratin-19)
0 (Keratin-7)
126880-72-6 (Cystic Fibrosis Transmembrane Conductance Regulator)
1393-25-5 (Secretin)
51110-01-1 (Somatostatin)
EC 2.3.2.2 (gamma-Glutamyltransferase)
تواريخ الأحداث: Date Created: 20170704 Date Completed: 20170911 Latest Revision: 20230201
رمز التحديث: 20230201
DOI: 10.1038/nm.4360
PMID: 28671689
قاعدة البيانات: MEDLINE
الوصف
تدمد:1546-170X
DOI:10.1038/nm.4360