دورية أكاديمية
Regulation of Small Mitochondrial DNA Replicative Advantage by Ribonucleotide Reductase in Saccharomyces cerevisiae .
| العنوان: | Regulation of Small Mitochondrial DNA Replicative Advantage by Ribonucleotide Reductase in Saccharomyces cerevisiae . |
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| المؤلفون: | Bradshaw E; Chemical Genetics Laboratory, RIKEN, Wako, Saitama 351-0198, Japan.; Core Research for Evolutional Science and Technology, Japan Agency for Medical Research and Development, Tokyo 100-0004, Japan.; Graduate School of Science and Engineering, Saitama University, 338-8570, Japan., Yoshida M; Chemical Genetics Laboratory, RIKEN, Wako, Saitama 351-0198, Japan.; Core Research for Evolutional Science and Technology, Japan Agency for Medical Research and Development, Tokyo 100-0004, Japan.; Graduate School of Science and Engineering, Saitama University, 338-8570, Japan., Ling F; Chemical Genetics Laboratory, RIKEN, Wako, Saitama 351-0198, Japan ling@postman.riken.go.jp.; Core Research for Evolutional Science and Technology, Japan Agency for Medical Research and Development, Tokyo 100-0004, Japan. |
| المصدر: | G3 (Bethesda, Md.) [G3 (Bethesda)] 2017 Sep 07; Vol. 7 (9), pp. 3083-3090. Date of Electronic Publication: 2017 Sep 07. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 101566598 Publication Model: Electronic Cited Medium: Internet ISSN: 2160-1836 (Electronic) Linking ISSN: 21601836 NLM ISO Abbreviation: G3 (Bethesda) Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [Oxford] : Oxford University Press Original Publication: Bethesda, MD : Genetics Society of America, 2011- |
| مواضيع طبية MeSH: | DNA Replication*, DNA, Mitochondrial/*genetics , DNA, Mitochondrial/*metabolism , Ribonucleotide Reductases/*metabolism , Saccharomyces cerevisiae/*genetics , Saccharomyces cerevisiae/*metabolism, Gene Expression ; Gene Expression Regulation, Fungal ; Mutation ; Phenotype ; Ribonucleotide Reductases/genetics ; Saccharomyces cerevisiae Proteins/genetics ; Saccharomyces cerevisiae Proteins/metabolism |
| مستخلص: | Small mitochondrial genomes can behave as selfish elements by displacing wild-type genomes regardless of their detriment to the host organism. In the budding yeast Saccharomyces cerevisiae , small hypersuppressive mtDNA transiently coexist with wild-type in a state of heteroplasmy, wherein the replicative advantage of the small mtDNA outcompetes wild-type and produces offspring without respiratory capacity in >95% of colonies. The cytosolic enzyme ribonucleotide reductase (RNR) catalyzes the rate-limiting step in dNTP synthesis and its inhibition has been correlated with increased petite colony formation, reflecting loss of respiratory function. Here, we used heteroplasmic diploids containing wild-type (rho + ) and suppressive (rho - ) or hypersuppressive (HS rho - ) mitochondrial genomes to explore the effects of RNR activity on mtDNA heteroplasmy in offspring. We found that the proportion of rho + offspring was significantly increased by RNR overexpression or deletion of its inhibitor, SML1, while reducing RNR activity via SML1 overexpression produced the opposite effects. In addition, using Ex Taq and KOD Dash polymerases, we observed a replicative advantage for small over large template DNA in vitro , but only at low dNTP concentrations. These results suggest that dNTP insufficiency contributes to the replicative advantage of small mtDNA over wild-type and cytosolic dNTP synthesis by RNR is an important regulator of heteroplasmy involving small mtDNA molecules in yeast. (Copyright © 2017 Bradshaw et al.) |
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| فهرسة مساهمة: | Keywords: DNA replication; heteroplasmy; mitochondrial mutations; ribonucleotide reductase; suppressive mtDNA |
| المشرفين على المادة: | 0 (DNA, Mitochondrial) 0 (SML1 protein, S cerevisiae) 0 (Saccharomyces cerevisiae Proteins) EC 1.17.4.- (Ribonucleotide Reductases) EC 1.17.4.- (Rnr1 protein, S cerevisiae) |
| تواريخ الأحداث: | Date Created: 20170719 Date Completed: 20180503 Latest Revision: 20201209 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC5592933 |
| DOI: | 10.1534/g3.117.043851 |
| PMID: | 28717049 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2160-1836 |
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| DOI: | 10.1534/g3.117.043851 |