دورية أكاديمية
In silico search of inhibitors of Streptococcus mutans for the control of dental plaque.
| العنوان: | In silico search of inhibitors of Streptococcus mutans for the control of dental plaque. |
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| المؤلفون: | Ochoa R; Programa de Estudio y Control de Enfermedades Tropicales-PECET, Facultad de Medicina, Universidad de Antioquia, SIU- Sede de Investigación Universitaria, Medellín, Colombia. Electronic address: rodrigo.ochoa@udea.edu.co., Martínez-Pabón MC; Laboratorio de Microbiología Bucal, Facultad de Odontología, Universidad de Antioquia, Área de la Salud, Medellín, Colombia. Electronic address: macemapa@hotmail.com., Arismendi-Echeverri MA; Laboratorio de Microbiología Bucal, Facultad de Odontología, Universidad de Antioquia, Área de la Salud, Medellín, Colombia. Electronic address: marismendiecheverri@gmail.com., Rendón-Osorio WL; Laboratorio de Microbiología Bucal, Facultad de Odontología, Universidad de Antioquia, Área de la Salud, Medellín, Colombia. Electronic address: sietewiller@hotmail.com., Muskus-López CE; Programa de Estudio y Control de Enfermedades Tropicales-PECET, Facultad de Medicina, Universidad de Antioquia, SIU- Sede de Investigación Universitaria, Medellín, Colombia. Electronic address: carmusk@yahoo.com. |
| المصدر: | Archives of oral biology [Arch Oral Biol] 2017 Nov; Vol. 83, pp. 68-75. Date of Electronic Publication: 2017 Jul 01. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Pergamon Press Country of Publication: England NLM ID: 0116711 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1506 (Electronic) Linking ISSN: 00039969 NLM ISO Abbreviation: Arch Oral Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford : Pergamon Press Original Publication: Oxford, New York [etc.] Pergamon Press. |
| مواضيع طبية MeSH: | Dental Plaque/*microbiology , Phosphoenolpyruvate Sugar Phosphotransferase System/*physiology , Streptococcus mutans/*drug effects , Streptococcus mutans/*enzymology , Streptococcus mutans/*pathogenicity, Biofilms/drug effects ; Dental Caries/microbiology ; Dental Caries/prevention & control ; Dental Plaque/drug therapy ; Genome ; Humans ; Protein Interaction Maps ; Streptococcus mutans/genetics ; Virulence/drug effects |
| مستخلص: | Biofilm is an extremely complex microbial community arranged in a matrix of polysaccharides and attached to a substrate. Its development is crucial in the pathophysiology of oral infections like dental caries, as well as in periodontal, pulp, and periapical diseases. Streptococcus mutans is one of the most effective microorganisms in lactic acid production of the dental biofilm. Identifying essential Streptococcus mutans proteins using bioinformatics methods helps to search for alternative therapies. To this end, the bacterial genomes of several Streptococcus mutans strains and representative strains of other cariogenic and non-cariogenic bacteria were analysed by identifying pathogenicity islands and alignments with other bacteria, and by detecting the exclusive genes of cariogenic species in comparison to the non-pathogenic ones. This study used tools for orthology prediction such as BLAST and OrthoMCL, as well as the server IslandViewer for the detection of pathogenicity islands. In addition, the potential interactome of Streptococcus mutans was rebuilt by comparing it to interologues of other species phylogenetically close to or associated with cariogenicity. This protocol yielded a final list of 20 proteins related to potentially virulent factors that can be used as therapeutic targets in future analyses. The EIIA and EIIC enzymatic subunits of the phosphotransferase system (PTS) were prioritized, as well as the pyruvate kinase enzyme, which are directly involved in the metabolism of carbohydrates and in obtaining the necessary energy for the microorganism's survival. These results will guide a subsequent experimental trial to develop new, safe, and effective molecules in the treatment of dental caries. (Copyright © 2017 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Bioinformatics; Dental caries; Molecular targets; Pathogenicity; Streptococcus mutans |
| المشرفين على المادة: | EC 2.7.1.- (Phosphoenolpyruvate Sugar Phosphotransferase System) |
| تواريخ الأحداث: | Date Created: 20170719 Date Completed: 20180625 Latest Revision: 20180625 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.archoralbio.2017.06.027 |
| PMID: | 28719833 |
| قاعدة البيانات: | MEDLINE |
Full Text via ClinicalKey 
Full Text Finder | تدمد: | 1879-1506 |
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| DOI: | 10.1016/j.archoralbio.2017.06.027 |