دورية أكاديمية
Effects of ERK1/2 kinases inactivation on the nigrostriatal system of Krushinsky-Molodkina rats genetically prone to audiogenic seizures.
| العنوان: | Effects of ERK1/2 kinases inactivation on the nigrostriatal system of Krushinsky-Molodkina rats genetically prone to audiogenic seizures. |
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| المؤلفون: | Dorofeeva NA; a Lab of Comparative Neurochemistry of Cellular Functions, Sechenov Institute of Evolutionary Physiology and Biochemistry , Russian Academy of Sciences , Saint-Petersburg , Russia., Grigorieva YS; a Lab of Comparative Neurochemistry of Cellular Functions, Sechenov Institute of Evolutionary Physiology and Biochemistry , Russian Academy of Sciences , Saint-Petersburg , Russia., Nikitina LS; a Lab of Comparative Neurochemistry of Cellular Functions, Sechenov Institute of Evolutionary Physiology and Biochemistry , Russian Academy of Sciences , Saint-Petersburg , Russia.; b Department of Biophysics , Saint-Petersburg State University , Saint-Petersburg , Russia., Lavrova EA; a Lab of Comparative Neurochemistry of Cellular Functions, Sechenov Institute of Evolutionary Physiology and Biochemistry , Russian Academy of Sciences , Saint-Petersburg , Russia., Nasluzova EV; a Lab of Comparative Neurochemistry of Cellular Functions, Sechenov Institute of Evolutionary Physiology and Biochemistry , Russian Academy of Sciences , Saint-Petersburg , Russia., Glazova MV; a Lab of Comparative Neurochemistry of Cellular Functions, Sechenov Institute of Evolutionary Physiology and Biochemistry , Russian Academy of Sciences , Saint-Petersburg , Russia., Chernigovskaya EV; a Lab of Comparative Neurochemistry of Cellular Functions, Sechenov Institute of Evolutionary Physiology and Biochemistry , Russian Academy of Sciences , Saint-Petersburg , Russia. |
| المصدر: | Neurological research [Neurol Res] 2017 Oct; Vol. 39 (10), pp. 918-925. Date of Electronic Publication: 2017 Jul 24. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Taylor & Francis Country of Publication: England NLM ID: 7905298 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1743-1328 (Electronic) Linking ISSN: 01616412 NLM ISO Abbreviation: Neurol Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2016- : Abingdon : Taylor & Francis Original Publication: New York, Crane, Russak. |
| مواضيع طبية MeSH: | Corpus Striatum/*enzymology , Mitogen-Activated Protein Kinase 1/*antagonists & inhibitors , Mitogen-Activated Protein Kinase 3/*antagonists & inhibitors , Seizures/*enzymology , Substantia Nigra/*enzymology, Acoustic Stimulation ; Aminoacetonitrile/analogs & derivatives ; Aminoacetonitrile/pharmacology ; Animals ; Auditory Perception/physiology ; Corpus Striatum/drug effects ; Disease Models, Animal ; Female ; Glutamic Acid/metabolism ; Male ; Mitogen-Activated Protein Kinase 1/metabolism ; Mitogen-Activated Protein Kinase 3/metabolism ; Neurons/drug effects ; Neurons/enzymology ; Protein Kinase Inhibitors/pharmacology ; Rats ; Seizures/drug therapy ; Seizures/etiology ; Substantia Nigra/drug effects ; Synapsins/metabolism ; gamma-Aminobutyric Acid/metabolism |
| مستخلص: | Recently, we demonstrated that inhibition of ERK1/2 activity by SL-327 treatment blocks seizure behavior in Krushinsky-Molodkina (KM) rats, which was mediated by altering of GABA and glutamate release mechanism in the hippocampus. Basal ganglia representing various subcortical cell groups play a significant role in the regulation of motor activity, including epileptiform seizures. Objectives: To verify if nigrostriatal system could be also affected by SL-327 treatment we analyzed the expression of tyrosine hydroxylase, D1 and D2 dopamine receptors, NR2B subunit of NMDA receptor as well as vesicular glutamate transporter VGLUT2 and glutamic acid decarboxylases GAD65/67 in the striatum and substantia nigra of KM rats. Methods: Animals were injected i.p. with SL-327 (50 mg/kg) 60 min before audio stimulation. After audiogenic stimulation the brains of control and SL 327 treated rats were removed for further immunohistochemical and biochemical analysis. Results: Obtained results demonstrated a decrease activity in synapsin I, and accumulation of VGLUT2 in the striatum after blockade of audiogenic seizure (AGS) by SL 327 that could lead to inhibition of glutamate release. While in the striatum GAD65/67 level was diminished, in the substantia nigra GAD65/67 was increased showing enhanced inhibitory output to the compact part of the substantia nigra. Analysis of dopaminergic system showed a significant reduction of tyrosine hydroxylase activity and expression in the substantia nigra, and decreased D1 and D2 receptor expression in the striatum. In summary, we propose that changes in the nigrostriatal system could be mediated by inhibitory effect of SL 327 on AGS expression. |
| فهرسة مساهمة: | Keywords: ERK1/2; GAD62/67; Krushinsky-Molodkina audiogenic rats; VGLUT2; striatum; substantia nigra; synapsin I |
| المشرفين على المادة: | 0 (Protein Kinase Inhibitors) 0 (SL 327) 0 (Synapsins) 3739OQ10IJ (Aminoacetonitrile) 3KX376GY7L (Glutamic Acid) 56-12-2 (gamma-Aminobutyric Acid) EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1) EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3) |
| تواريخ الأحداث: | Date Created: 20170726 Date Completed: 20180515 Latest Revision: 20180515 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1080/01616412.2017.1356156 |
| PMID: | 28738742 |
| قاعدة البيانات: | MEDLINE |
PDF full text from Publisher | تدمد: | 1743-1328 |
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| DOI: | 10.1080/01616412.2017.1356156 |