دورية أكاديمية
أعرض في EDS

Mechanical loading disrupts osteocyte plasma membranes which initiates mechanosensation events in bone.

التفاصيل البيبلوغرافية
العنوان: Mechanical loading disrupts osteocyte plasma membranes which initiates mechanosensation events in bone.
المؤلفون: Yu K; Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, 1120 15th Street, Augusta, 30912, Georgia., Sellman DP; Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, 1120 15th Street, Augusta, 30912, Georgia., Bahraini A; Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, 1120 15th Street, Augusta, 30912, Georgia., Hagan ML; Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, 1120 15th Street, Augusta, 30912, Georgia., Elsherbini A; Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, 1120 15th Street, Augusta, 30912, Georgia., Vanpelt KT; Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, 1120 15th Street, Augusta, 30912, Georgia., Marshall PL; Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, 1120 15th Street, Augusta, 30912, Georgia., Hamrick MW; Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, 1120 15th Street, Augusta, 30912, Georgia., McNeil A; Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, 1120 15th Street, Augusta, 30912, Georgia., McNeil PL; Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, 1120 15th Street, Augusta, 30912, Georgia., McGee-Lawrence ME; Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, 1120 15th Street, Augusta, 30912, Georgia.; Department of Orthopaedic Surgery, Medical College of Georgia, Augusta University, 1120 15th Street, Augusta, Georgia.
المصدر: Journal of orthopaedic research : official publication of the Orthopaedic Research Society [J Orthop Res] 2018 Feb; Vol. 36 (2), pp. 653-662. Date of Electronic Publication: 2017 Aug 11.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: Wiley Country of Publication: United States NLM ID: 8404726 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1554-527X (Electronic) Linking ISSN: 07360266 NLM ISO Abbreviation: J Orthop Res Subsets: MEDLINE
أسماء مطبوعة: Publication: 2006- : Hoboken, NJ : Wiley
Original Publication: New York, N.Y. : Raven Press, [c1983-
مواضيع طبية MeSH: Mechanotransduction, Cellular*, Bone and Bones/*physiology , Osteocytes/*physiology, Actin Cytoskeleton/metabolism ; Animals ; Apoptosis ; Calcium/metabolism ; Cell Line ; Mice ; Microfluidic Analytical Techniques ; Primary Cell Culture ; Stress, Mechanical ; Weight-Bearing
مستخلص: Osteocytes sense loading in bone, but their mechanosensation mechanisms remain poorly understood. Plasma membrane disruptions (PMD) develop with loading under physiological conditions in many cell types (e.g., myocytes, endothelial cells). These PMD foster molecular flux across cell membranes that promotes tissue adaptation, but this mechanosensation mechanism had not been explored in osteocytes. Our goal was to investigate whether PMD occur and initiate consequent mechanotransduction in osteocytes during physiological loading. We found that osteocytes experience PMD during in vitro (fluid flow) and in vivo (treadmill exercise) mechanical loading, in proportion to the level of stress experienced. In fluid flow studies, osteocyte PMD preferentially formed with rapid as compared to gradual application of loading. In treadmill studies, osteocyte PMD increased with loading in weight bearing locations (tibia), but this trend was not seen in non-weight bearing locations (skull). PMD initiated osteocyte mechanotransduction including calcium signaling and expression of c-fos, and repair rates of these PMD could be enhanced or inhibited pharmacologically to alter downstream mechanotransduction and osteocyte survival. PMD may represent a novel mechanosensation pathway in bone and a target for modifying skeletal adaptation signaling in osteocytes. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:653-662, 2018.
(© 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.)
References: J Biol Chem. 2015 Jul 3;290(27):16744-58. (PMID: 25953900)
J Bone Miner Res. 2016 Jan;31(1):116-28. (PMID: 26211746)
Curr Biol. 2009 Aug 25;19(16):1389-95. (PMID: 19631537)
J Musculoskelet Neuronal Interact. 2010 Mar;10(1):64-70. (PMID: 20190381)
J Bone Miner Res. 1997 Jan;12(1):45-51. (PMID: 9240724)
Med Eng Phys. 2015 Aug;37(8):719-28. (PMID: 26117332)
Biochem Biophys Res Commun. 2014 May 23;448(1):83-8. (PMID: 24780398)
Biomech Model Mechanobiol. 2014 Jan;13(1):85-97. (PMID: 23567965)
Med Sci Sports Exerc. 2001 Aug;33(8):1318-23. (PMID: 11474333)
J Bone Miner Res. 2015 Mar;30(3):436-48. (PMID: 25270829)
Calcif Tissue Int. 2012 May;90(5):429-37. (PMID: 22466445)
Mol Biol Cell. 2005 Jul;16(7):3100-6. (PMID: 15843434)
J Exp Biol. 2015 Oct;218(Pt 19):3002-9. (PMID: 26232415)
Bone. 2012 Sep;51(3):459-65. (PMID: 22750014)
Proc Natl Acad Sci U S A. 2004 Nov 23;101(47):16689-94. (PMID: 15539460)
J Biol Chem. 2007 Dec 7;282(49):35868-77. (PMID: 17942392)
J Bone Miner Res. 2014 Apr;29(4):878-91. (PMID: 24115222)
Diabetes. 2011 Nov;60(11):3034-43. (PMID: 21940783)
J Biomech. 1994 Mar;27(3):339-60. (PMID: 8051194)
Ann Biomed Eng. 2005 Jan;33(1):104-10. (PMID: 15709711)
Bone. 2010 Nov;47(5):872-81. (PMID: 20713195)
J Cell Physiol. 2007 Jul;212(1):207-14. (PMID: 17301958)
Int J Sports Med. 1998 May;19(4):245-9. (PMID: 9657363)
Physiol Rev. 2015 Oct;95(4):1205-40. (PMID: 26336031)
Rev Endocr Metab Disord. 2015 Jun;16(2):115-29. (PMID: 26038304)
J Biomech. 2010 Oct 19;43(14):2765-70. (PMID: 20674920)
Cell Calcium. 2015 Dec;58(6):638-48. (PMID: 26572560)
J Neurosci. 2013 Mar 20;33(12):5085-94. (PMID: 23516275)
Nature. 2003 May 8;423(6936):168-72. (PMID: 12736685)
Methods Mol Med. 2003;80:381-98. (PMID: 12728733)
Microsc Microanal. 2012 Dec;18(6):1430-41. (PMID: 23171702)
Arthritis Rheum. 2009 Nov;60(11):3336-45. (PMID: 19877030)
J Biomech. 2014 Jun 3;47(8):1838-45. (PMID: 24720889)
J Orthop Res. 2013 Jul;31(7):1075-81. (PMID: 23483620)
Nat Commun. 2011 Dec 20;2:597. (PMID: 22186893)
Dev Dyn. 2007 Jan;236(1):240-50. (PMID: 17117435)
Muscles Ligaments Tendons J. 2015 Feb 05;4(4):413-9. (PMID: 25767777)
J Appl Physiol (1985). 1990 Jan;68(1):130-4. (PMID: 2312452)
Free Radic Biol Med. 2015 Jul;84:246-253. (PMID: 25843658)
J Bone Miner Res. 2012 Mar;27(3):563-74. (PMID: 22113822)
Cell Calcium. 2010 Mar;47(3):234-41. (PMID: 20060586)
Mol Biol Cell. 1999 Apr;10(4):1247-57. (PMID: 10198070)
J Biomech. 1996 Nov;29(11):1411-7. (PMID: 8894921)
Proc Natl Acad Sci U S A. 2010 Aug 3;107(31):13648-53. (PMID: 20643964)
Am J Pathol. 1992 May;140(5):1097-109. (PMID: 1374591)
Calcif Tissue Int. 2015 Jan;96(1):65-72. (PMID: 25398598)
J Biomech. 2009 Aug 25;42(12):1989-95. (PMID: 19625024)
Proc Natl Acad Sci U S A. 2007 Aug 14;104(33):13325-30. (PMID: 17673554)
J Membr Biol. 2012 Jul;245(7):401-9. (PMID: 22797941)
Am J Physiol Cell Physiol. 2004 Aug;287(2):C475-83. (PMID: 15084473)
معلومات مُعتمدة: P01 AG036675 United States AG NIA NIH HHS
فهرسة مساهمة: Keywords: bone; cell wounding; mechanical loading; mechanotransduction; osteocyte; skeleton
المشرفين على المادة: SY7Q814VUP (Calcium)
تواريخ الأحداث: Date Created: 20170730 Date Completed: 20190319 Latest Revision: 20240313
رمز التحديث: 20240313
مُعرف محوري في PubMed: PMC5788741
DOI: 10.1002/jor.23665
PMID: 28755471
قاعدة البيانات: MEDLINE
الوصف
تدمد:1554-527X
DOI:10.1002/jor.23665