دورية أكاديمية
The Duffy null genotype is associated with a lower level of CCL2, leukocytes and neutrophil count but not with the clinical outcome of HTLV-1 infection.
| العنوان: | The Duffy null genotype is associated with a lower level of CCL2, leukocytes and neutrophil count but not with the clinical outcome of HTLV-1 infection. |
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| المؤلفون: | da Silva-Malta MCF; Serviço de Pesquisa, Fundação Hemominas, Belo Horizonte, Minas Gerais, Brazil.; Interdisciplinary HTLV Research Group (GIPH), Brazil., Sales CC; Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas, Gerais, Brazil., Guimarães JC; Instituto de Ciências Biológicas, Universidade Federal de Minas, Gerais, Belo Horizonte, Brazil., de Cássia Gonçalves P; Serviço de Pesquisa, Fundação Hemominas, Belo Horizonte, Minas Gerais, Brazil., Chaves DG; Serviço de Pesquisa, Fundação Hemominas, Belo Horizonte, Minas Gerais, Brazil.; Interdisciplinary HTLV Research Group (GIPH), Brazil., Santos HC; Laboratório de Genética e Cardiologia Molecular, Instituto do Coração, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., da Costa Pereira A; Laboratório de Genética e Cardiologia Molecular, Instituto do Coração, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., Ribas JG; Interdisciplinary HTLV Research Group (GIPH), Brazil., de Freitas Carneiro-Proietti AB; Serviço de Pesquisa, Fundação Hemominas, Belo Horizonte, Minas Gerais, Brazil.; Interdisciplinary HTLV Research Group (GIPH), Brazil., Martins ML; Interdisciplinary HTLV Research Group (GIPH), Brazil.; Serviço de Pesquisa, Fundação Hemominas, Belo Horizonte, Minas Gerais, Brazil. |
| المصدر: | Journal of medical microbiology [J Med Microbiol] 2017 Aug; Vol. 66 (8), pp. 1207-1216. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Microbiology Society Country of Publication: England NLM ID: 0224131 Publication Model: Print Cited Medium: Internet ISSN: 1473-5644 (Electronic) Linking ISSN: 00222615 NLM ISO Abbreviation: J Med Microbiol Subsets: PubMed not MEDLINE; MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : London : Microbiology Society Original Publication: 1968-1995: Edinburgh, Livingstone. |
| مستخلص: | Purpose. Chemokines are important in the immune response against viral infections, and may play a role in human T-lymphotropic virus 1 (HTLV-1)-associated myelopathy/tropical spastic paraparesis (HAM/TSP) pathogenesis. Polymorphisms in the Duffy antigen receptor for chemokines (DARC), such as rs12075 (A>G; FY*B>FY*A ) and rs281477 (-46T>C; GATA-1 box) may influence circulating concentrations of proinflammatory chemokines. We investigate whether Duffy genotypes influence the HTLV-1 proviral load (PVL) level, HTLV-1 infection outcome and chemokine concentrations in HTLV-1 asymptomatic carriers (AC=162), HAM/TSP patients (HAM=135) and seronegative individuals (SN=71). Methodology. Quantification of plasmatic IL8, CCL2 and CCL5 were performed by flow cytometry and Duffy genotypes were investigated by real-time PCR. HTLV-1 PVL was quantified in peripheral blood. To control for spurious association, individual ancestry profiles in AC and HAM groups were investigated. Results/Key findings. PVL and IL8 level were significantly higher in the HAM group than in the AC group, but were not associated with Duffy genotypes. The highest CCL2 and CCL5 levels were seen in the SN group, and there was no difference when comparing the infected groups. The level of CCL5 was not associated with Duffy genotypes. The polymorphism -46 C/C that abrogates the DARC expression on the erythrocytes was significantly associated with lower levels of CCL2, neutrophil and white blood cell (WBC) counts in HTLV-1-infected individuals. Conclusion. We conclude that although the Duffy null genotype was associated with leukopenia, neutropenia and lower levels of CCL2, the data do not suggest the influence of Duffy genotypes on the neurologic outcome of HTLV-1 infection, but may be a confounding factor in comparison HTLV-1-infected populations with different ancestries, especially when defining inflammatory biomarkers. |
| فهرسة مساهمة: | Keywords: DARC; Duffy; HAM/TSP; HTLV-1; chemokines; proviral load |
| تواريخ الأحداث: | Date Created: 20170804 Latest Revision: 20200108 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1099/jmm.0.000539 |
| PMID: | 28771137 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1473-5644 |
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| DOI: | 10.1099/jmm.0.000539 |